LOCUS       sequence-36716-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Koc EC, Spremulli LL
  TITLE     Identification of mammalian mitochondrial translational initiation 
            factor 3 and examination of its role in initiation complex formation
            with natural mRNAs.
  JOURNAL   J Biol Chem. 2002 Sep 20. 277(38):35541-9.
  PUBMED    12095986
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2002 Sep 20. 277(38):35541-9."
COMMENT     Sequence Label: pET21-E. coli Initiation Factor 3 (IF3)
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             535..546
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             589..606
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(659..677)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      673..720
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(844..863)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tctagaaata attttgttta actttaagaa ggagatatac atatgaaagg cggaaaacga
       61 gttcaaacgg cgcgccctaa ccgtatcaat ggcgaaattc gcgcccagga agttcgctta
      121 acaggtctgg aaggcgagca gcttggtatt gtgagtctga gagaagctct ggagaaagca
      181 gaagaagccg gagtagactt agtcgagatc agccctaacg ccgagccgcc ggtttgtcgt
      241 ataatggatt acggcaaatt cctctatgaa aagagcaagt cttctaagga acagaagaaa
      301 aagcaaaaag ttatccaggt taaggaaatt aaattccgtc ctggtacaga tgaaggcgac
      361 tatcaggtaa aactccgcag cctgattcgc tttctcgaag agggtgataa agccaaaatc
      421 acgctgcgtt tccgcggtcg tgagatggcg caccagcaaa tcggtatgga agtgcttaat
      481 cgcgtgaaag acgatttgca agaactggca gtggtcgaat ccttcccaac gaagatcgaa
      541 ggccgccaga tgatcatggt gctcgctcct aagaagaaac agctcgagca ccaccaccac
      601 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      661 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      721 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
      781 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      841 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt cc
//
