LOCUS       sequence-367172-        2602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-TT3
FEATURES             Location/Qualifiers
     source          1..2602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     435..451
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     435..451
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     456..475
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        456..474
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(649..1839)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1468..1487
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1887..1892,1893..1909,1910..1915))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(join(2042..2602,1..28))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2122..2141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
       61 cgctcagtgg aacgacgcgt aactcacgtt aagggatttt ggtcatgggt ggctcgacca
      121 gatatggtct cactacaact tgctcgggcg tcaggccgtg ggcttgacag agaactggta
      181 gaaggcgctg aacagtctca agagcctgtt tgccaccccc attactagca atcgctacaa
      241 cttgctcggg cgtcaggccg tgggcttgac agagaactgg tagaaggcgc tgaacagtct
      301 caagagcctg tttgccaccc ccattactag caatcgctac cacgagacca gctttggtct
      361 agaggaggca tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca
      421 cgggccagag ctgccaggaa acagctatga ccatgtaata cgactcacta taggggatat
      481 tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt
      541 tcttccccga tcaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc
      601 caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt
      661 ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc
      721 tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat
      781 cagcggtcca atgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc
      841 ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc
      901 gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag
      961 caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa
     1021 acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac
     1081 cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac
     1141 ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc
     1201 ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa
     1261 gggcatgggt cggcgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag
     1321 gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgtaaggaga tggcgcccaa
     1381 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
     1441 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
     1501 acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagagaatcc acaggacggg
     1561 tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg
     1621 gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa
     1681 cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc
     1741 ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac
     1801 ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta
     1861 gcaatttaac tgtgataaac taccgcatta aagctaatcg atgataagct gtcaaacatg
     1921 agaattaatt catgttcttt cctgcgttat cccctgattc tgtggataac gcaggaaaga
     1981 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2041 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2101 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2161 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2221 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2281 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2341 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2401 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2461 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     2521 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2581 gtttttttgt ttgcaagcag ca
//
