LOCUS       sequence-367182-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GG8
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     536..552
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     536..552
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     557..576
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        557..575
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(750..1940)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1569..1588
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1988..1993,1994..2010,2011..2016))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(join(2143..2600,1..131))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2223..2242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
       61 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
      121 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgacg
      181 cgtaactcac gttaagggat tttggtcatg ggtggctcga ccagatatgg tctcgaagta
      241 acaatggtgg caaacaggct cttgaaaccg tacagcgcct actgccagtt ctctgtcaag
      301 cccacggtct gactccggag caagttgtag cgattgctag taacaatggt ggcaaacagg
      361 ctcttgaaac cgtacagcgc ctactgccag ttctctgtca agcccacggt ctgactccgg
      421 agcaagttgt agcgattgct tcctgagacc agctttggtc tagaggaggc atgatgatat
      481 atttttatct tgtgcaatgt aacatcagag attttgagac acgggccaga gctgccagga
      541 aacagctatg accatgtaat acgactcact ataggggata ttttattttc tcccaatcag
      601 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atcaagggtt
      661 ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa
      721 tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg
      781 cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg
      841 ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc aatgatcgaa
      901 gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc
      961 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
     1021 ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg
     1081 tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca
     1141 gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc
     1201 atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc
     1261 tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc
     1321 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatggg tcggcgctct
     1381 cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
     1441 gccgccgcaa ggaatggtgc atgtaaggag atggcgccca acagtccccc ggccacgggg
     1501 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
     1561 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
     1621 ccggccacga tgcgtccggc gtagagaatc cacaggacgg gtgtggtcgc catgatcgcg
     1681 tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg
     1741 gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc
     1801 acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt
     1861 accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg
     1921 agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa
     1981 ctaccgcatt aaagctaatc gatgataagc tgtcaaacat gagaattaat tcatgttctt
     2041 tcctgcgtta tcccctgatt ctgtggataa cgcaggaaag aacatgtgag caaaaggcca
     2101 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2161 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2221 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2281 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2341 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2401 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2461 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2521 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2581 aagaacagta tttggtatct
//
