LOCUS       sequence-36719-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Emdadul Haque M, Grasso D, Miller C, Spremulli LL, Saada A
  TITLE     The effect of mutated mitochondrial ribosomal proteins S16 and S22 
            on the assembly of the small and large ribosomal subunits in human 
            mitochondria.
  JOURNAL   Mitochondrion. 2008 Jun . 8(3):254-61.
  PUBMED    18539099
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Mitochondrion. 2008 Jun . 8(3):254-61."
COMMENT     Sequence Label: pET21-MRPS2
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             799..816
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(869..887)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgat ccgcgagtcg
       61 gaggacagca ccgatttcaa cgacaagatt ttgaatgagc ccctcaagca ctctgacttc
      121 ttcaatgtca aggaactgtt ttccgtgaga agcctcttcg atgcccgagt ccatctggga
      181 cacaaagctg gctgtcggca caggtttatg gagccgtaca tctttgggag ccgcctggac
      241 cacgacatca tcgacctgga acagacagcc acgcacctcc agctggcctt gaacttcacc
      301 gcccacatgg cctaccgcaa gggcatcatc ttgtttataa gccgcaaccg gcagttctcg
      361 tacctgattg agaacatggc ccgtgactgt ggcgagtacg cccacactcg ctacttcagg
      421 ggcggcatgc tgaccaacgc gcgcctcctc tttggcccca cggtccgcct gccggacctc
      481 atcatcttcc tgcacacgct caacaacatc tttgagccac acgtggccgt gagagacgca
      541 gccaagatga acatccccac agtgggcatc gtggacacca actgcaaccc ctgcctcatc
      601 acctaccctg tacccggcaa tgacgactct ccgctggctg tgcacctcta ctgcaggctc
      661 ttccagacgg ccatcacccg ggccaaggag aagcggcagc aggttgaggc tctctatcgc
      721 ctgcagggcc agaaggagcc cggggaccag gggccagccc accctcctgg ggctgacatg
      781 agccattccc tgctcgagca ccaccaccac caccactgag atccggctgc taacaaagcc
      841 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
      901 gcctctaaac gggtcttgag gggttt
//
