LOCUS       sequence-367191-        8984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mitochondrial targetted (COX8) mCerulean.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8984)
  AUTHORS   Balderas E, Eberhardt DR, Lee S, Pleinis JM, Sommakia S, Balynas AM,
            Yin X, Parker MC, Maguire CT, Cho S, Szulik MW, Bakhtina A, Bia RD, 
            Friederich MW, Locke TM, Van Hove JLK, Drakos SG, Sancak Y, 
            Tristani-Firouzi M, Franklin S, Rodan AR, Chaudhuri D
  TITLE     Mitochondrial calcium uniporter stabilization preserves energetic 
            homeostasis during Complex I impairment.
  JOURNAL   Nat Commun. 2022 May 19;13(1):2769. doi: 10.1038/s41467-022-30236-4.
  PUBMED    35589699
REFERENCE   2  (bases 1 to 8984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-19"; volume: "13(1):2769. doi"; pages: " 
            10.1038/s41467-022-30236-4"
COMMENT     Sequence Label: pLenti-mitoX4-mCerulean
FEATURES             Location/Qualifiers
     source          1..8984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1714..2017
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2018..2221
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2172..2192
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2218..2242
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2304..2378
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             2400..2474
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             2496..2570
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             2592..2666
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             join(2691..2693,2694..2696,2697..3410)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2736..2757)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2997..3016)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3344..3365
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3417..4005
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3470..3490)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3888..3899)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4019..4518
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4054..4077)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4451..4470
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4539..5138
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4539..4558)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5035..5055
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5276..5509
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5581..5702
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5618..5637)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5672..5691
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5742..5877
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5897..5916)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5898..5916)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5926..5943)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5926..5942)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5935..5957)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6084..6539
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6171..6190)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6381..6402
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6565..6669
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6670..6738,6739..7530)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6888..6907)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7701..8289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8190..8209
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8443..8460
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8577..8598
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8613..8618,8619..8636,8637..8643)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8651..8667
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8656..8678
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8675..8691
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8675..8691
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8710..8730
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8712..8730
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8758..8984
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc
     1741 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1801 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1861 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1921 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1981 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2041 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2101 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2161 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2221 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     2281 aagacaccga ctctagagcc accatgagcg tgctgacacc attgttgctc agaggcctca
     2341 ctggttcagc caggcggctg ccagtcccaa gagctaagat ccatagtctg ggagatccaa
     2401 tgtcagttct cactcccctt ttgcttaggg gactgaccgg aagcgctcgg cggttgcctg
     2461 tcccacgcgc caagattcac tccctgggcg accctatgag tgtccttact cctcttttgc
     2521 tgagaggctt gacagggagc gctcggaggc tcccagtgcc tcgcgcaaag atccattctc
     2581 tcggagatcc catgtccgtc ctcactcctc tgctgctgcg cgggcttaca ggttccgctc
     2641 gccgcctgcc tgttcctcgg gcaaagatac acagtctggg ggatcctgcc atggtgagca
     2701 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     2761 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     2821 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     2881 ccctgacctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     2941 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     3001 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     3061 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     3121 acaacgccat cagcgacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     3181 ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     3241 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     3301 cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     3361 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa gtcgacaatc
     3421 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     3481 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     3541 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     3601 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     3661 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     3721 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     3781 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     3841 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     3901 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     3961 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attctaccgg
     4021 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     4081 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     4141 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     4201 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     4261 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     4321 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     4381 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     4441 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     4501 tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg
     4561 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     4621 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     4681 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     4741 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     4801 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     4861 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     4921 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     4981 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     5041 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     5101 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc
     5161 gcccgaccga aaggagcgca cgaccccatg catctcgagg gcccggtacc tttaagacca
     5221 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     5281 gggctagctc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     5341 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     5401 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     5461 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     5521 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     5581 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5641 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5701 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     5761 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5821 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5881 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     5941 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     6001 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     6061 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6121 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     6181 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     6241 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     6301 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     6361 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     6421 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     6481 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     6541 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     6601 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     6661 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     6721 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     6781 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     6841 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     6901 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     6961 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7021 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     7081 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     7141 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     7201 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     7261 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     7321 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     7381 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     7441 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     7501 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     7561 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     7621 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7681 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7741 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7801 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     7861 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7921 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7981 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8041 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8101 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8161 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8221 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8281 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     8341 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     8401 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8461 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     8521 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     8581 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     8641 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     8701 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
     8761 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     8821 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     8881 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     8941 attgcagaga tattgtattt aagtgcctag ctcgatacat aaac
//
