LOCUS       sequence-367192-        8817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_Ollas.S.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        143..247
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(248..316,317..1108)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(466..485)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1279..1867
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1768..1787
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2021..2038
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2155..2176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2191..2196,2197..2214,2215..2221)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2229..2245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2234..2256
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2253..2269
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2253..2269
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2288..2308
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2290..2308
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2336..2562
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2563..2743
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2790..2915
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3408..3641
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3826..3870
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4168..4416
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4168..4188
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4339..4358
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4435..4510
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4558..4675
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4815..5993
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5046..5984
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5941..5961
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6042..6051
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6054..6095
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             6117..6161
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             6180..6221
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6231..6938
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6376..6394)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6644..6663
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7008..7596
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7061..7081)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7479..7490)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7671..7904
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7976..8097
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8013..8032)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8067..8086
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8137..8272
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8292..8311)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8293..8311)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8321..8338)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8321..8337)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8330..8352)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8479..8817,1..117)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8566..8585)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8776..8797
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
       61 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
      121 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
      181 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      241 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
      301 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
      361 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
      421 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
      481 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
      541 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
      601 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
      661 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
      721 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
      781 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
      841 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
      901 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
      961 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     1021 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     1081 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     1141 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     1201 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     1261 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1321 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1381 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1441 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1501 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1561 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1621 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1681 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1741 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1801 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     1861 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     1921 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     1981 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     2041 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     2101 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     2161 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     2221 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     2281 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     2341 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     2401 caaggagaga aaaagcaccg tgcatggcga ttggtggaag taaggtggta cgatcgtgcc
     2461 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     2521 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     2581 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     2641 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     2701 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     2761 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     2821 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     2881 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     2941 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     3001 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     3061 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     3121 aacttagatc attatataat acagtagcra ccctctattg tgtgcatcaa aggatagaga
     3181 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     3241 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     3301 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     3361 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     3421 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     3481 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     3541 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     3601 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     3661 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     3721 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     3781 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     3841 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     3901 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     3961 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     4021 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     4081 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg atcacgagac
     4141 tagtatcgag cggccgcccc cttcaccgag ggcctatttc ccatgattcc ttcatatttg
     4201 catatacgat acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag
     4261 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     4321 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     4381 ttggctttat atatcttgtg gaaaggacga aacaccgggt cttcgagaag acctgtttta
     4441 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
     4501 gagtcggtgc tttttttaat tctcgacctc gagacaaatg gcagtattca tccacaattt
     4561 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     4621 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     4681 ttattacagg gacagcagag atccactttg gccgcggctc gaggcgataa gctttgcaaa
     4741 gatggataaa gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga
     4801 aaggagtggg aattggctcc ggtgcccgtc agtgggcmga gcgcacatcg cccacagtcc
     4861 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg
     4921 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     4981 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     5041 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt
     5101 gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg
     5161 ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt
     5221 gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
     5281 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
     5341 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt
     5401 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
     5461 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
     5521 ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
     5581 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga
     5641 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
     5701 aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt
     5761 ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg
     5821 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt
     5881 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc
     5941 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggaattc
     6001 tgcagtcgat cgacggtacc gcgggcccgg gatccacggt cgccaccatg ggcagcggct
     6061 tcgccaacga gctgggcccc aggctgatgg gcaagtgcgg aggaggcggg ggagggaagg
     6121 agaccgccgc cgccaagttc gagaggcagc acatggacag cggaggaggc gggggagggg
     6181 gcaaaccaat tccaaaccca ctgctgggac tggattccac aggcatgctg atggtgagca
     6241 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     6301 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     6361 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     6421 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     6481 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     6541 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     6601 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     6661 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     6721 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     6781 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     6841 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     6901 gccgccactc caccggcggc atggacgagc tgtacaagaa acggccagcc gaggatatgg
     6961 aggaagaaca agcctttaaa cgatctcgat aaagcggccg cgtcgacaat caacctctgg
     7021 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     7081 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     7141 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     7201 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     7261 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     7321 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     7381 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     7441 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     7501 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcatcttcgc cttcgccctc
     7561 agacgagtcg gatctccctt tgggccgcct ccccgcctgg gtacctttaa gaccaatgac
     7621 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7681 aattcactcc caacgaagat aagatctgct ttttgcttgt actgggtctc tctggttaga
     7741 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7801 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7861 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     7921 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     7981 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     8041 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     8101 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8161 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8221 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacs
     8281 tacccaatts gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     8341 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     8401 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     8461 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     8521 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     8581 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     8641 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     8701 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     8761 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatct
//
