LOCUS       sequence-36720-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Emdadul Haque M, Grasso D, Miller C, Spremulli LL, Saada A
  TITLE     The effect of mutated mitochondrial ribosomal proteins S16 and S22 
            on the assembly of the small and large ribosomal subunits in human 
            mitochondria.
  JOURNAL   Mitochondrion. 2008 Jun . 8(3):254-61.
  PUBMED    18539099
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Mitochondrion. 2008 Jun . 8(3):254-61."
COMMENT     Sequence Label: pET21-MRPS11
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             553..570
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(623..641)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      637..684
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(808..827)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgcc ggccgggacc
       61 atctgcacag gcgctcgaca gctccaagac gctgcggcca agcagaaagt tgaacagaac
      121 gcggctccca gccacaccaa gttcagcatt taccctccca ttccaggaga ggagagctct
      181 ctgaggtggg caggaaagaa atttgaggag atcccaattg cacacattaa agcatcccac
      241 aacaacacac agatccaggt agtctctgct agtaatgagc cccttgcctt tgcttcctgt
      301 ggcacagagg gatttcggaa tgccaagaag ggcacaggca tcgcagcaca gacagcaggc
      361 atagccgcag cggcgagagc taaacaaaag ggcgtgatcc acatccgagt tgtggtgaaa
      421 ggcctggggc caggacgctt gtctgccatg cacggactga tcatgggcgg cctggaagtg
      481 atctcaatca cagacaacac cccaatccca cacaacggct gccgccccag gaaggctcgg
      541 aagctgctcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
      601 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
      661 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg
      721 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      781 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      841 cgttcgccgg ctttccccgt caagctctaa atcggg
//
