LOCUS       sequence-36721-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Tournier C, Whitmarsh AJ, Cavanagh J, Barrett T, Davis RJ
  TITLE     Mitogen-activated protein kinase kinase 7 is an activator of the 
            c-Jun NH2-terminal kinase.
  JOURNAL   Proc Natl Acad Sci U S A. 1997 Jul 8. 94(14):7337-42.
  PUBMED    9207092
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1997 Jul 8. 94(14):7337-42."
COMMENT     Sequence Label: pCDNA3 Flag MKK7a1 (ala)
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             104..127
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgngggcttt gcgactctct ggctactgag acccactgct tactggctta tcgaaattaa
       61 tacgactcac tatagggaga cccaagcttc ggcagccaac atggactata aggacgatga
      121 tgacaaaatg ctggggctcc catcaacctt gttcacaccg cgcagtatgg agagcatcga
      181 gattgaccag aagctgcagg agatcatgaa gcagacaggg tacctgacta tcgggggcca
      241 gcgttatcag gcagaaatca atgacttgga gaacttgggt gagatgggca gtggtacctg
      301 tggtcaggtg tggaagatgc ggttccggaa gacaggccac atcattgctg ttaagcaaat
      361 gcggcgctct gggaacaagg aagagaataa gcgcattttg atggacctgg atgtagtact
      421 caagagccat gactgccctt acatcgttca gtgctttggc accttcatca ccaacacaga
      481 cgtctttatt gccatggagc tcatgggcac atgtgcagag aagctgaaga aacgaatgca
      541 gggccccatt ccagagcgaa tcctgggcaa gatgactgtg gcgattgtga aagcactgta
      601 ctatctgaag gagaagcatg gcgtcatcca tcgcgatgtc aaaccctcca acatcctgct
      661 agatgagcgg ggccagatca agctctgtga ctttggcatc agtggccgcc ttgttgacgc
      721 caaagccaaa gcacggagtg ctggctgtgc tgcctatatg gctcccgagc gcatcgaccc
      781 tccagatccc accaagcctg actatgacat ccgagctgat gtgtggagcc tgggcatctc
      841 actggtggag ctggcaacag gacagttccc ctataagaac tgcaagacgg actttgagtc
      901 ctcaccaagt cctacagaaa agccccactc ctgctgctgt cagtctc
//
