LOCUS       sequence-367218-       10737 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human KIF5A(∆exon27) and human KLC1 in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Nakano J, Chiba K, Niwa S
  TITLE     An ALS-associated KIF5A mutant forms oligomers and aggregates and 
            induces neuronal toxicity.
  JOURNAL   Genes Cells. 2022 Apr 17. doi: 10.1111/gtc.12936.
  PUBMED    35430760
REFERENCE   2  (bases 1 to 10737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Cells. 2022 Apr 17. doi: 10.1111/gtc.12936."
COMMENT     Sequence Label: pSN840
FEATURES             Location/Qualifiers
     source          1..10737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(172..177,178..194,195..200))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(271..304)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    506..554
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     579..598
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(2362..2385)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2386..2403)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(2442..2551)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             complement(2642..3433)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3898..4254
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(4256..4289)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4366..4457
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4382..4399
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             join(8332..8355,8356..8391,8392..8415)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     polyA_signal    8564..8698
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8601..8620)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8655..8674
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  8819..8984
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      9174..9762
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9663..9682
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  9897..10120
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(10187..10720)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg catagactgt
       61 acaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
      121 tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga
      181 accgaacagg cttatgtcaa ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac
      241 ccggcaacct tgggcagcag cgaagtcgcc ataacttcgt atagcataca ttatacgaag
      301 ttatctgcca ggcacatggg tttaaacgta cccgtagtgg ctatggcagg gcttgccgcc
      361 ccgacgttgg ctgcgagccc tgggccttca cccgaacttg ggggttgggg tggggaaaag
      421 gaagaaacgc gggcgtattg gtcccaatgg ggtctcggtg gggtatcgac agagtgccag
      481 ccctgggacc gaaccccgcg tttatgaaca aacgacccaa cacccgtgcg ttttattctg
      541 tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt
      601 tagcctcccc catctcccgg taccgcatgc tatgcatcag ctgctagcac catggctcga
      661 gatccctcag ttaaccagcc cgagcttcat tttcctcatc ccgttccact ctacgctcat
      721 actcacttcc tcccctccgt cagggccact ctcgtacttg accacgtcca cgttgaggct
      781 ctcacggctc ctgcgcttct ccatgttctc agggtcattg agcacttctg ccaccctctg
      841 tttgtgaaca ttgtcaagac cctgtttacg agacctcata gcagcttctt ctaacgtttc
      901 tgcagcttca aatttgcctt gacgtctgta aagtgcccca aggtttttta gagtggttgt
      961 aacagttgga ctatcaactt tgcaggcttt gtaccagccg ccatactctc caaaagatgt
     1021 cccatccttt tgctttcctt tgcattcttc tctttcttca gcatgcatcc agatgggttt
     1081 attttcatca tctacagaac caaactccct ttcatgtgca cgagtgagaa tctctttgta
     1141 cagtgtttct gcttgcttga actttccttg tttcaaatag caggatgcca ggttattttt
     1201 cgtcttagcc acgttggggt catcaggtcc cagttttgtc tggtagatct cgagggctct
     1261 ttgataataa tattctactt cttcatactt gccctggttc tggcacagta aggccaagtt
     1321 atttaactgc ttggcaacat cggggtgatc cttccccaaa accttttctc ggatttccag
     1381 agctctttta cacaacggct ctgcttcttt gtacttccct cttttaccat aaaggactgc
     1441 aaggttattc aaagtcgccg ccaccgcagg atgatctttg cccaaagttt tctcacgaat
     1501 agccaaggca tcattcagta ggttagctgc atctttgtat ttattctgat ccctgtacac
     1561 caaggccagg atgttgagca tggtggccac gtccgggtgg tcgtgtcctg aagtcttctc
     1621 caggtcctcc agggcctgct tgcagagggg cacagctacc tcgtagcgcc cctgcgaggc
     1681 gtactggatc accaggttgt ggagcgtccg cagccgcgcg gggatctcgt agccgccctg
     1741 ctgggcagcc gcggctgcac tgctgtgctg ctgctggatt ccttgccctg ggtcgtcttc
     1801 atcattgggg aaaaggtcat ccagaggctc tttggtagaa tcagtgtctt tgtcctcgga
     1861 tggggaaatg tcgtcatcat atttttttag ctgattcata aactccagat gcttcttctc
     1921 ctcctccagt tgagccacag actgctcact cttctgcagt ttctgctgcg tgttggccag
     1981 ttcatcccgt agccactgat tctcctggca cagacgacga acctgcgcac gcagtttctg
     2041 cttctcggac tccacagcat tcaggtgatt tgacaaagcc atcataacct gtgcctcact
     2101 caggccgagc tccaacatct ccagtgactt ccggatcatg tttgatttct cctccaccaa
     2161 attactttca tcatctttct tcaaacactt cagtgtctcc agcaaacttt gtaaaatgga
     2221 attgtgctca ttcttcaaag cttccagccc ctgaattact tgctttgtct tagaaataat
     2281 ttcatcctgt gtaagcttct ccaacttgtc ttcctttatg tacaccattg tggacatgtt
     2341 gtcatacata ctgccactgc ccttgtcatc gtcatccttg tagtcgtgat ggtgatggtg
     2401 atggtagtac gacatggtgg gtgatcaagt cttcgtcgag tgattgtaaa taaaatgtaa
     2461 tttacagtat agtattttaa ttaatataca aatgatttga taataattct tatttaacta
     2521 taatatattg tgttgggttg aattaaaggt ccgtatacta gtatcgatac ctaggtgcca
     2581 tttcattacc tctttctccg cacccgacat agatccgtcg gcttgaacga attgttagac
     2641 attatttgcc gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact
     2701 gatctgcgcg cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa
     2761 gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct
     2821 tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat
     2881 ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg
     2941 cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca
     3001 aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg
     3061 ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc
     3121 gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag
     3181 cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag
     3241 ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt
     3301 gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt
     3361 cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca
     3421 ccgcttccct catgatgttt aactttgttt tagggcgact gccctgctgc gtaacatcgt
     3481 tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc
     3541 gaggcataga ctgtacccca aaaaaacagt cataacaagc catgaaaacc gccactgcgc
     3601 cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact
     3661 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     3721 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     3781 ccagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtgttccg
     3841 cttcctttag cagcccttgc gccctgagtg cttgcggcag cgtgaagcta attcccatgt
     3901 cagccgttaa gtgttcctgt gtcactcaaa attgctttga gaggctctaa gggcttctca
     3961 gtgcgttaca tccctggctt gttgtccaca accgttaaac cttaaaagct ttaaaagcct
     4021 tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa gttgctgatt
     4081 tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag agcttagtac
     4141 gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa catgagagct
     4201 tagtacgtta aacatgagag cttagtacgt gaaacatgag agcttagtac gtaccataac
     4261 ttcgtatagc atacattata cgaagttatc tgtaactata acggtcctaa ggtagcgagt
     4321 ttaaacacta gtatcgattc gcgacctact ccggaatatt aatagatcat ggagataatt
     4381 aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa
     4441 taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg cgcgatggcg
     4501 gagaccaaca acgaatgtag catcaaggtg ctctgccgat tccggcccct gaaccaggct
     4561 gagattctgc ggggagacaa gttcatcccc attttccaag gggacgacag cgtcgttatt
     4621 ggggggaagc catatgtttt tgaccgtgta ttccccccaa acacgactca agagcaagtt
     4681 tatcatgcat gtgccatgca gattgtcaaa gatgtccttg ctggctacaa tggcaccatt
     4741 tttgcttatg gacagacatc ctcagggaaa acacatacca tggagggaaa gctgcacgac
     4801 cctcagctga tgggaatcat tcctcgaatt gcccgagaca tcttcaacca catctactcc
     4861 atggatgaga accttgagtt ccacatcaag gtttcttact ttgaaattta cctggacaaa
     4921 attcgtgacc ttctggatgt gaccaagaca aatctgtccg tgcacgagga caagaaccgg
     4981 gtgccatttg tcaagggttg tactgaacgc tttgtgtcca gcccggagga gattctggat
     5041 gtgattgatg aagggaaatc aaatcgtcat gtggctgtca ccaacatgaa tgaacacagc
     5101 tctcggagcc acagcatctt cctcatcaac atcaagcagg agaacatgga aacggagcag
     5161 aagctcagtg ggaagctgta tctggtggac ctggcaggga gtgagaaggt cagcaagact
     5221 ggagcagagg gagccgtgct ggacgaggca aagaatatca acaagtcact gtcagctctg
     5281 ggcaatgtga tctccgcact ggctgagggc actaaaagct atgttccata tcgtgacagc
     5341 aaaatgacaa ggattctcca ggactctctc gggggaaact gccggacgac tatgttcatc
     5401 tgttgctcac catccagtta taatgatgca gagaccaagt ccaccctgat gtttgggcag
     5461 cgggcaaaga ccattaagaa cactgcctca gtaaatttgg agttgactgc tgagcagtgg
     5521 aagaagaaat atgagaagga gaaggagaag acaaaggccc agaaggagac gattgcgaag
     5581 ctggaggctg agctgagccg gtggcgcaat ggagagaatg tgcctgagac agagcgcctg
     5641 gctggggagg aggcagccct gggagccgag ctctgtgagg agacccctgt gaatgacaac
     5701 tcatccatcg tggtgcgcat cgcgcccgag gagcggcaga aatacgagga ggagatccgc
     5761 cgtctctata agcagcttga cgacaaggat gatgaaatca accaacaaag ccaactcata
     5821 gagaagctca agcagcaaat gctggaccag gaagagctgc tggtgtccac ccgaggagac
     5881 aacgagaagg tccagcggga gctgagccac ctgcaatcag agaacgatgc cgctaaggat
     5941 gaggtgaagg aagtgctgca ggccctggag gagctggctg tgaactatga ccagaagtcc
     6001 caggaggtgg aggagaagag ccagcagaac cagcttctgg tggatgagct gtctcagaag
     6061 gtggccacca tgctgtccct ggagtctgag ttgcagcggc tacaggaggt cagtggacac
     6121 cagcgaaaac gaattgctga ggtgctgaac gggctgatga aggatctgag cgagttcagt
     6181 gtcattgtgg gcaacgggga gattaagctg ccagtggaga tcagtggggc catcgaggag
     6241 gagttcactg tggcccgact ctacatcagc aaaatcaaat cagaagtcaa gtctgtggtc
     6301 aagcggtgcc ggcagctgga gaacctccag gtggagtgtc accgcaagat ggaagtgacc
     6361 gggcgggagc tctcatcctg ccagctcctc atctctcagc atgaggccaa gatccgctcg
     6421 cttacggaat acatgcagag cgtggagcta aagaagcggc acctggaaga gtcctatgac
     6481 tccttgagcg atgagctggc caagctccag gcccaggaaa ctgtgcatga agtggccctg
     6541 aaggacaagg agcctgacac tcaggatgca gatgaagtga agaaggctct ggagctgcag
     6601 atggagagtc accgggaggc ccatcaccgg cagctggccc ggctccggga cgagatcaac
     6661 gagaagcaga agaccattga tgagctcaaa gacctaaatc agaagctcca gttagagcta
     6721 gagaagcttc aggctgacta cgagaagctg aagagcgaag aacacgagaa gagcaccaag
     6781 ctgcaggagc tgacatttct gtacgagcga catgagcagt ccaagcagga cctcaagggt
     6841 ctggaggaga cagttgcccg ggaactccag accctccaca accttcgcaa gctgttcgtt
     6901 caagacgtca cgactcgagt caagaaaagt gcagaaatgg agcccgaaga cagtgggggg
     6961 attcactccc aaaagcagaa gatttccttt cttgagaaca acctggaaca gcttacaaag
     7021 gttcacaaac agctggtacg tgacaatgca gatctgcgtt gtgagcttcc taaattggaa
     7081 aaacgactta gggctacggc tgagagagtt aaggccctgg agggtgcact gaaggaggcc
     7141 aaggagggcg ccatgaagga caagcgccgg taccagcagg aggtggaccg catcaaggag
     7201 gccgttcgct acaagagctc gggcaaacgg ggccattctg cccagattgc caaacccgtc
     7261 cggcctggcc actacccagc atcctcaccc accaacccct atggcacccg gagccctgag
     7321 tgcatcagtt acaccaacag cctcttccag aactaccaga atctctacct gcaggccaca
     7381 cccagctcca cctcagatat gtactttgca aactcctgta ccagcagtgg agccacatct
     7441 tctggcggcc ccttggcttc ctaccagaag gccaacatgg acaatggagt gacctgccgt
     7501 gtggctatga ggctgaggac caggccaagc ttttccctct ccaccaagag acagcagcca
     7561 gctaatctcc cacacccacg gctgcatacc tgcactttca gtttcgggct ttcgaatatg
     7621 gtcagcaaag gtgaagcggt aataaaggag ttcatgcgct tcaaggtgca catggaaggt
     7681 agcatgaatg gacatgagtt tgagattgag ggagaaggtg agggcagacc atacgaagga
     7741 acacagacgg ctaagctcaa agtgaccaag ggaggcccgt tgcctttctc ttgggacatt
     7801 cttagtcccc aattcatgta tggaagtaga gccttcacga aacatccggc ggatataccc
     7861 gattattaca agcagtcctt tcccgaggga ttcaaatggg agcgtgtaat gaattttgag
     7921 gatggaggcg cagtaacagt tacccaagac acttcactgg aggatggtac acttatttac
     7981 aaagtcaaac ttcgcggcac taattttccg cctgacggtc ccgttatgca gaaaaaaaca
     8041 atgggatggg aagcctcgac tgaacgtctg taccctgagg acggcgtcct gaaaggtgac
     8101 attaaaatgg ctctgcgcct caaagacggt ggacgttacc ttgctgactt taaaactact
     8161 tacaaagcaa aaaaaccggt acaaatgccc ggcgcgtaca acgtggatcg caagttggac
     8221 attacttctc ataacgagga ttacaccgtc gttgagcagt atgagaggag cgaaggccgc
     8281 catagcactg gaggaatgga tgaattgtat aaactcgagg gcagtggcag ttggtcgcat
     8341 cctcaattcg agaagggcgg cggcagtggc ggcggcagtg gcggcagtgc ctggagtcat
     8401 ccacaatttg agaagtaaag cctgcagtct cgacaagctt gtcgagaagt actagaggat
     8461 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     8521 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     8581 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8641 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg
     8701 atcactgctt gagcctagaa gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     8761 ctgctgccac cgctgagcaa taactatcat aacccctagg gtatacccat ctaattggaa
     8821 ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta
     8881 cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact
     8941 ccatttccac ccctcccagt tcccaactat tttgtccgcc cacaaccggt tgacttgggt
     9001 caactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     9061 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     9121 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     9181 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     9241 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     9301 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     9361 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     9421 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     9481 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     9541 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     9601 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     9661 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9721 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9781 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9841 cctgattgac ttgggtcgct cttcctgtgg atgcgcagat gccctgcgta agcgggtgtg
     9901 ggcggacaat aaagtcttaa actgaacaaa atagatctaa actatgacaa taaagtctta
     9961 aactagacag aatagttgta aactgaaatc agtccagtta tgctgtgaaa aagcatactg
    10021 gacttttgtt atggctaaag caaactcttc attttctgaa gtgcaaattg cccgtcgtat
    10081 taaagagggg cgtggccaag ggcatgtaaa gactatattc gcggcgttgt gacaatttac
    10141 cgaacaactc cgcggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac
    10201 ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca
    10261 ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg
    10321 cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg
    10381 agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg ggcagaacgt
    10441 aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct
    10501 tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta
    10561 cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag
    10621 ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc
    10681 gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct ccataac
//
