LOCUS       sequence-36722-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Tournier C, Whitmarsh AJ, Cavanagh J, Barrett T, Davis RJ
  TITLE     Mitogen-activated protein kinase kinase 7 is an activator of the 
            c-Jun NH2-terminal kinase.
  JOURNAL   Proc Natl Acad Sci U S A. 1997 Jul 8. 94(14):7337-42.
  PUBMED    9207092
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1997 Jul 8. 94(14):7337-42."
COMMENT     Sequence Label: pCDNA3 Flag MKK7a1 (glu)
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             107..130
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgngggctna ncgagctctc tggctactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttcggcagcc aacatggact ataaggacga
      121 tgatgacaaa atgctggggc tcccatcaac cttgttcaca ccgcgcagta tggagagcat
      181 cgagattgac cagaagctgc aggagatcat gaagcagaca gggtacctga ctatcggggg
      241 ccagcgttat caggcagaaa tcaatgactt ggagaacttg ggtgagatgg gcagtggtac
      301 ctgtggtcag gtgtggaaga tgcggttccg gaagacaggc cacatcattg ctgttaagca
      361 aatgcggcgc tctgggaaca aggaagagaa taagcgcatt ttgatggacc tggatgtagt
      421 actcaagagc catgactgcc cttacatcgt tcagtgcttt ggcaccttca tcaccaacac
      481 agacgtcttt attgccatgg agctcatggg cacatgtgca gagaagctga agaaacgaat
      541 gcagggcccc attccagagc gaatcctggg caagatgact gtggcgattg tgaaagcact
      601 gtactatctg aaggagaagc atggcgtcat ccatcgcgat gtcaaaccct ccaacatcct
      661 gctagatgag cggggccaga tcaagctctg tgactttggc atcagtggcc gccttgttga
      721 cgaaaaagcc aaagaacgga gtgctggctg tgctgcctat atggctcccg agcgcatcga
      781 ccctccagat cccaccaagc ctgactatga catccgagct gatgtgtgga gcctgggcat
      841 ctcactggtg gagctggcaa caggacagtt cccctataag aactgcaaga cggactttga
      901 ggtcctcacc aaagtcctac aggaagagcc cccactcctg cctggtcaca tgggcttntc
      961 agatctctnc aa
//
