LOCUS       sequence-367223-         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Construct for labelling actin filaments in mammalian cells with 
            lifeact-mScarlet3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Gadella TWJ Jr, van Weeren L, Stouthamer J, Hink MA, Wolters AHG, 
            Giepmans BNG, Aumonier S, Dupuy J, Royant A
  TITLE     mScarlet3: a brilliant and fast-maturing red fluorescent protein.
  JOURNAL   Nat Methods. 2023 Apr;20(4):541-545. doi: 
            10.1038/s41592-023-01809-y. Epub 2023 Mar 27.
  PUBMED    36973546
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-023-01809-y"; journalName: "Nat Methods"; date: 
            "2023-04"; volume: "20"; issue: "4"; pages: "541-545"
COMMENT     Sequence Label: pLifeAct-mScarlet3_N1
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..51
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      67..76
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             73..759
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     471..490
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgggcgtgg ccgacctgat caagaagttc gagagcatca gcaaggagga gggggatcca
       61 ccggtcgcca ccatggatag caccgaggca gtgatcaagg agttcatgcg gttcaaggtg
      121 cacatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      181 ccctacgagg gcacccagac cgccaagctg agggtgacca agggtggccc cctgcccttc
      241 tcctgggaca tcctgtcccc tcagttcatg tacggctcca gggccttcac gaagcacccc
      301 gccgacatcc ccgactactg gaagcagtcc ttccccgagg gcttcaagtg ggagcgcgtg
      361 atgaacttcg aggacggcgg cgccgtgtcc gtggcccagg acacctccct ggaggacggc
      421 accctgatct acaaggtgaa gctccgcggc accaacttcc ctcctgacgg ccccgtaatg
      481 cagaagaaga caatgggctg ggaagcatcc accgagcggt tgtaccccga ggacgtcgtg
      541 ctgaagggcg acattaagat ggccctgcgc ctgaaggacg gcggccgcta cctggcggac
      601 ttcaagacca cctacagggc caagaagccc gtgcagatgc ccggcgcctt caacatcgac
      661 cgcaagttgg acatcacatc ccacaacgag gactacaccg tggtggaaca gtacgaacgc
      721 tccgtggccc gccactccac cggcggctcc ggtggctcct aa
//
