LOCUS       sequence-367238-        1730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use 
            in bi-cistronic and as the first 2A peptide in tri-cistronic 
            construct assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Mukherjee M, Wang ZQ
  TITLE     A Well-Characterized Polycistronic-Like Gene Expression System in 
            Yeast.
  JOURNAL   Biotechnol Bioeng. 2022 Sep 27. doi: 10.1002/bit.28247.
  PUBMED    36168285
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2022 Sep 27. doi: 10.1002/bit.28247."
COMMENT     Sequence Label: pYTK001_O2Ab
FEATURES             Location/Qualifiers
     source          1..1730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     362..381
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(657..691)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(762..1421)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1336..1355
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1603..1730,1..461)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
       61 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      121 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      181 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      241 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      301 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      361 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      421 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      481 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
      541 ctgattctgt ggataaccgt agtcggggtc tcggttctgg taagtccaat tacgacctat
      601 tgaagttgtg tggagatgtt gaatccaacc caggacctag gagaccgacc agaccaataa
      661 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
      721 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
      781 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
      841 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
      901 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
      961 gtgaaactca cccagggatt ggctgaaacg aaaaacatat tctcaataaa ccctttaggg
     1021 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     1081 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     1141 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     1201 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     1261 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     1321 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     1381 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     1441 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     1501 ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt
     1561 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1621 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1681 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
//
