LOCUS       sequence-367249-       22370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA for creating transgenic plants expressing Cas9 and Drm1b 
            gRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22370)
  AUTHORS   Weiss T, Wang C, Kang X, Zhao H, Elena Gamo M, Starker CG, Crisp PA,
            Zhou P, Springer NM, Voytas DF, Zhang F
  TITLE     Optimization of multiplexed CRISPR/Cas9 system for highly efficient 
            genome editing in Setaria viridis.
  JOURNAL   Plant J. 2020 Nov;104(3):828-838. doi: 10.1111/tpj.14949. Epub 2020 
            Sep 8.
  PUBMED    32786122
REFERENCE   2  (bases 1 to 22370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.14949";
            journalName: "Plant J"; date: "2020-11"; volume: "104"; issue: "3"; 
            pages: "828-838"
COMMENT     Sequence Label: pTW037
FEATURES             Location/Qualifiers
     source          1..22370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1827..2852)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    4792..4813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4828..4833,4834..4851,4852..4858)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4866..4882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4871..4893
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4890..4906
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4890..4906
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7671..7727
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7728..11831
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7728..11831
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11832..11879
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_RNA        12740..12815
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     polyA_signal    12912..13086
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             14996..16648
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(15062..15080)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      16658..16906
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(17381..17398)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(17381..17397)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17390..17412)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    17600..17624
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             18925..19554
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             19983..21056
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      21122..21316
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(21552..21574)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    21660..21800
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     21715..21734
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(21815..21832)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(21986..22370,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(22066..22085)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgacagatc ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt
     1861 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg
     1921 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
     1981 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca
     2041 agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc
     2101 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg
     2161 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt
     2221 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg
     2281 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca
     2341 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat
     2401 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg
     2461 ctcgtctggc taagatcggc cgcagcgatc gcatccatag cctccgcgac cggttgtaga
     2521 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtga acggcgggag
     2581 atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc
     2641 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta
     2701 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
     2761 ccctccgaga gctgcatcag gtcggacacg ctgtcgaact tttcgatcag aaacttctcg
     2821 acagacgtcg cggtgagttc aggctttttc ataagcttct gcaaaagaga accagacaac
     2881 agggtaagtg cctagcagta aacaaacaga actcatcaca agcaaacagc aacatcatat
     2941 tcataccaac aggtcatgtg tgttcatcac atcattagta ctaagcatgc catcatccaa
     3001 gtatatcaaa gtaagggcaa agagcatgca tgatcatcag gtgcacaaaa gaatcatcaa
     3061 attgtagcag tacatatctt catctatcat gcatatctat ccataacagg acgatgcatg
     3121 ttgaccaggt aaaagctaca ggatcctata ggaacagcag cgtatatatc ttcaccaatc
     3181 ttagcatctt aatcatgtgg cacatgcagt ttcaatttaa gcacatgagc tagttgatta
     3241 tgaggtacca gagaatcatc aaactgatgt agcagcatat atcctcatct atcatgcaat
     3301 ctaatctaat ctaactaaac aggaaaggtg tgctattcag ttaaaagcta ccgcatcata
     3361 tacaaacggc agcataagaa aaagcataat catcttaatc atgcaacaaa cgcagattca
     3421 taataagccc aagagctagc ttgtgatgat cttattctac tctgatctac agcaatcaga
     3481 taacgaccta accttgcaca tggcaacaaa acaatcgatc ggacgaatca gttgtttgtt
     3541 cctagctagc accatcgaac cagataatag atgcacgtac agatcccgaa aacgaaccca
     3601 aaaacagggc agacctagct gaacctaggc agcgacccag cagatcgtga gaacgatctc
     3661 atctacgaac agcctagaag caaccccacg attcccggac aaacgaccta aaatccccac
     3721 aaatcacatg agcatgacag gataaacagc ggaaccgatc agatctacac gaaaacccca
     3781 cctcccagcc acccacgatc aggaaacacg cggatctagc atgatttcgt caacgcctca
     3841 gcctagttcc tagccacaga ccaagcagaa ccaccaaacc acgccgagcg aggagatggg
     3901 gcaagaggac gggggagacg atcgccgtac cttgaagcgg gggaaggatc gccgagggtc
     3961 gcgaggagag caatttggat ttggaacccg ggggttgtgc gctccgaacg atgagacgat
     4021 gtgagattgt gggaagaggc gcggagggcc ctgtatttat gggctgcgac ggggggagga
     4081 gaggtgggga gggttgggga aggaatcccc cacccgtgcc gtgacggttc cgggccgtgt
     4141 gagaggagcc cgctcgtctc cgccacgcaa tttccgcgat cggagcggag ctttcgagag
     4201 gcggctggat ggttggtggc cgttagattt gtagacgccg ttaacgcctc gcctccaccg
     4261 ggaagagttt tgagcagccg cttatgacaa tggcttaacg acgttagacg gagcgttagt
     4321 ggcaggccat gatagaatag acgtattgca atgggattat aattaaataa ataagaatat
     4381 aataagatat ggcaagtcgg cactcatgac atggtcttcg aaatgatagt gctcactttc
     4441 ttagccgaga aagttgacgc gactgattta gaagttaaga tattatttct ctcttctttt
     4501 ctttcctcgt catataagga tgaaataaac tttagagatt gccggtagtg attttggatt
     4561 tcggcgatca ggcttggttt gccggtttcg gacggtgtgc cttaggccac ccgcagtgta
     4621 tcttgtaatg ttcaaccgat aagcaagggt ggggctcaag caagtagtaa acaactatgt
     4681 caaatgtcac catggttatg gtcttgttta gttggcttct tggccgattc attaatgcag
     4741 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     4801 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     4861 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc
     4921 gagctcggta cccggggatc tgcaggcgcg cctgcagtgc agcgtgaccc ggtcgtgccc
     4981 ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt
     5041 tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa actttactct
     5101 acgaataata taatctatag tactacaata atatcagtgt tttagagaat catataaatg
     5161 aacagttaga catggtctaa aggacaattg agtattttga caacaggact ctacagtttt
     5221 atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac
     5281 ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa
     5341 tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct
     5401 attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa gtgactaaaa
     5461 attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag
     5521 tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc
     5581 agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg
     5641 acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt
     5701 gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc
     5761 accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc
     5821 gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt tcggagcgca
     5881 cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt caaggtacgc
     5941 cgctcgtcct cccccccccc cctctctacc ttctctagat cggcgttccg gtccatggtt
     6001 agggcccggt agttctactt ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc
     6061 gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca gacacgttct gattgctaac
     6121 ttgccagtgt ttctctttgg ggaatcctgg gatggctcta gccgttccgc agacgggatc
     6181 gatttcatga ttttttttgt ttcgttgcat agggtttggt ttgccctttt cctttatttc
     6241 aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat gctttttttt gtcttggttg
     6301 tgatgatgtg gtctggttgg gcggtcgttc tagatcggag tagaattaat tctgtttcaa
     6361 actacctggt ggatttatta attttggatc tgtatgtgtg tgccatacat attcatagtt
     6421 acgaattgaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg
     6481 ttttactgat gcatatacag agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg
     6541 ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgt
     6601 atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac gagtttaaga
     6661 tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata
     6721 tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc tatctattat
     6781 aataaacaag tatgttttat aattattttg atcttgatat acttggatga tggcatatgc
     6841 agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt gcttggtact
     6901 gtttcttttg tcgatgctca ccctgttgtt tggtgttact tcctgcagga agcatgtctg
     6961 agccacctcg ggctgagacc tttgtattcc tggacctaga agccactggg ctcccaaaca
     7021 tggaccctga gattgcagag atatcccttt ttgctgttca ccgctcttcc ctggagaacc
     7081 cagaacggga tgattctggt tccttggtgc tgccccgtgt tctggacaag ctcacactgt
     7141 gcatgtgccc ggagcgcccc tttactgcca aggccagtga gattactggt ttgagcagcg
     7201 aaagcctgat gcactgcggg aaggctggtt tcaatggcgc tgtggtaagg acactgcagg
     7261 gcttcctaag ccgccaggag ggccccatct gccttgtggc ccacaatggc ttcgattatg
     7321 acttcccact gctgtgcacg gagctacaac gtctgggtgc ccatctgccc caagacactg
     7381 tctgcctgga cacactgcct gcattgcggg gcctggaccg tgctcacagc cacggcacca
     7441 gggctcaagg ccgcaaaagc tacagcctgg ccagtctctt ccaccgctac ttccaggctg
     7501 aacccagtgc tgcccattca gcagaaggtg atgtgcacac cctgcttctg atcttcctgc
     7561 atcgtgctcc tgagctgctc gcctgggcag atgagcaggc ccgcagctgg gctcatattg
     7621 agcccatgta cgtgccacct gatggtccaa gcctcgaagc cggctccggc gccaccaact
     7681 tctccctcct caagcaagcc ggcgacgtgg aggagaaccc aggcccaatg gacaagaagt
     7741 actcgatcgg cctcgacatc gggacgaact cagttggctg ggccgtgatc accgacgagt
     7801 acaaggtgcc ctctaagaag ttcaaggtcc tggggaacac cgaccgccat tccatcaaga
     7861 agaacctcat cggcgctctc ctgttcgaca gcggggagac cgctgaggct acgaggctca
     7921 agagaaccgc taggcgccgg tacacgagaa ggaagaacag gatctgctac ctccaagaga
     7981 ttttctccaa cgagatggcc aaggttgacg attcattctt ccaccgcctg gaggagtctt
     8041 tcctcgtgga ggaggataag aagcacgagc ggcatcccat cttcggcaac atcgtggacg
     8101 aggttgccta ccacgagaag taccctacga tctaccatct gcggaagaag ctcgtggact
     8161 ccaccgataa ggcggacctc agactgatct acctcgctct ggcccacatg atcaagttcc
     8221 gcggccattt cctgatcgag ggggatctca acccagacaa cagcgatgtt gacaagctgt
     8281 tcatccaact cgtgcagacc tacaaccaac tcttcgagga gaacccgatc aacgcctctg
     8341 gcgtggacgc gaaggctatc ctgtccgcga ggctctcgaa gtccaggagg ctggagaacc
     8401 tgatcgctca gctcccaggc gagaagaaga acggcctgtt cgggaacctc atcgctctca
     8461 gcctggggct caccccgaac ttcaagtcga acttcgatct cgctgaggac gccaagctgc
     8521 aactctccaa ggacacctac gacgatgacc tcgataacct cctggcccag atcggcgatc
     8581 aatacgcgga cctgttcctc gctgccaaga acctgtcgga cgccatcctc ctgtcagata
     8641 tcctccgcgt gaacaccgag atcacgaagg ctccactctc tgcctccatg atcaagcgct
     8701 acgacgagca ccatcaggat ctgaccctcc tgaaggcgct ggtccgccaa cagctcccgg
     8761 agaagtacaa ggagattttc ttcgatcagt cgaagaacgg ctacgctggg tacatcgacg
     8821 gcggggcctc acaagaggag ttctacaagt tcatcaagcc aatcctggag aagatggacg
     8881 gcacggagga gctcctggtg aagctcaaca gggaggacct cctgcggaag cagagaacct
     8941 tcgataacgg cagcatcccc caccaaatcc atctcgggga gctgcacgcc atcctgagaa
     9001 ggcaagagga cttctaccct ttcctcaagg ataaccggga gaagatcgag aagatcctga
     9061 ccttcagaat cccatactac gtcggccctc tcgcgcgggg gaactcaaga ttcgcttgga
     9121 tgacccgcaa gtctgaggag accatcacgc cgtggaactt cgaggaggtg gtggacaagg
     9181 gcgctagcgc tcagtcgttc atcgagagga tgaccaactt cgacaagaac ctgcccaacg
     9241 agaaggtgct ccctaagcac tcgctcctgt acgagtactt caccgtctac aacgagctca
     9301 cgaaggtgaa gtacgtcacc gagggcatgc gcaagccagc gttcctgtcc ggggagcaga
     9361 agaaggctat cgtggacctc ctgttcaaga ccaaccggaa ggtcacggtt aagcaactca
     9421 aggaggacta cttcaagaag atcgagtgct tcgattcggt cgagatcagc ggcgttgagg
     9481 accgcttcaa cgccagcctc gggacctacc acgatctcct gaagatcatc aaggataagg
     9541 acttcctgga caacgaggag aacgaggata tcctggagga catcgtgctg accctcacgc
     9601 tgttcgagga cagggagatg atcgaggagc gcctgaagac gtacgcccat ctcttcgatg
     9661 acaaggtcat gaagcaactc aagcgccgga gatacaccgg ctgggggagg ctgtcccgca
     9721 agctcatcaa cggcatccgg gacaagcagt ccgggaagac catcctcgac ttcctcaaga
     9781 gcgatggctt cgccaacagg aacttcatgc aactgatcca cgatgacagc ctcaccttca
     9841 aggaggatat ccaaaaggct caagtgagcg gccaggggga ctcgctgcac gagcatatcg
     9901 cgaacctcgc tggctccccc gcgatcaaga agggcatcct ccagaccgtg aaggttgtgg
     9961 acgagctcgt gaaggtcatg ggccggcaca agcctgagaa catcgtcatc gagatggcca
    10021 gagagaacca aaccacgcag aaggggcaaa agaactctag ggagcgcatg aagcgcatcg
    10081 aggagggcat caaggagctg gggtcccaaa tcctcaagga gcacccagtg gagaacaccc
    10141 aactgcagaa cgagaagctc tacctgtact acctccagaa cggcagggat atgtacgtgg
    10201 accaagagct ggatatcaac cgcctcagcg attacgacgt cgatcatatc gttccccagt
    10261 ctttcctgaa ggatgactcc atcgacaaca aggtcctcac caggtcggac aagaaccgcg
    10321 gcaagtcaga taacgttcca tctgaggagg tcgttaagaa gatgaagaac tactggaggc
    10381 agctcctgaa cgccaagctg atcacgcaaa ggaagttcga caacctcacc aaggctgaga
    10441 gaggcgggct ctcagagctg gacaaggccg gcttcatcaa gcggcagctg gtcgagacca
    10501 gacaaatcac gaagcacgtt gcgcaaatcc tcgactctcg gatgaacacg aagtacgatg
    10561 agaacgacaa gctgatcagg gaggttaagg tgatcaccct gaagtctaag ctcgtctccg
    10621 acttcaggaa ggatttccag ttctacaagg ttcgcgagat caacaactac caccatgccc
    10681 atgacgctta cctcaacgct gtggtcggca ccgctctgat caagaagtac ccaaagctgg
    10741 agtccgagtt cgtgtacggg gactacaagg tttacgatgt gcgcaagatg atcgccaagt
    10801 cggagcaaga gatcggcaag gctaccgcca agtacttctt ctactcaaac atcatgaact
    10861 tcttcaagac cgagatcacg ctggccaacg gcgagatccg gaagagaccg ctcatcgaga
    10921 ccaacggcga gacgggggag atcgtgtggg acaagggcag ggatttcgcg accgtccgca
    10981 aggttctctc catgccccag gtgaacatcg tcaagaagac cgaggtccaa acgggcgggt
    11041 tctcaaagga gtctatcctg cctaagcgga acagcgacaa gctcatcgcc agaaagaagg
    11101 actgggaccc aaagaagtac ggcgggttcg acagccctac cgtggcctac tcggtcctgg
    11161 ttgtggcgaa ggttgagaag ggcaagtcca agaagctcaa gagcgtgaag gagctcctgg
    11221 ggatcaccat catggagagg tccagcttcg agaagaaccc aatcgacttc ctggaggcca
    11281 agggctacaa ggaggtgaag aaggacctga tcatcaagct cccgaagtac tctctcttcg
    11341 agctggagaa cggcaggaag agaatgctgg cttccgctgg cgagctccag aaggggaacg
    11401 agctcgcgct gccaagcaag tacgtgaact tcctctacct ggcttcccac tacgagaagc
    11461 tcaagggcag cccggaggac aacgagcaaa agcagctgtt cgtcgagcag cacaagcatt
    11521 acctcgacga gatcatcgag caaatctccg agttcagcaa gcgcgtgatc ctcgccgacg
    11581 cgaacctgga taaggtcctc tccgcctaca acaagcaccg ggacaagccc atcagagagc
    11641 aagcggagaa catcatccat ctcttcaccc tgacgaacct cggcgctcct gctgctttca
    11701 agtacttcga caccacgatc gatcggaaga gatacacctc cacgaaggag gtcctggacg
    11761 cgaccctcat ccaccagtcg atcaccggcc tgtacgagac gaggatcgac ctctcacaac
    11821 tcggcgggga taagagaccc gcagcaacca agaaggcagg gcaagcaaag aagaagaagt
    11881 gactcgagta tatgaagatg aatatgaaat atttggtgtg tcaaataaaa agcttgtgtg
    11941 cttaagtttg tgtttttttc ttggcttgtt gtgttatgaa tttgtggctt tttctaatat
    12001 taaatgaatg taagatcaca ttataatgaa taaacaaatg tttctataat ccattgtgaa
    12061 tgttttgttg gatctcttct gcagcatata actactgtat gtgctatggt atggactatg
    12121 gaatatgatt aaagataagg agctcgttaa ccggtgacgg acggcgcgct ggcagacata
    12181 ctgtcccaca aatgaagatg gaatctgtaa aagaaaacgc gtgaaataat gcgtctgaca
    12241 aaggttaggt cggctgcctt taatcaatac caaagtggtc cctaccacga tggaaaaact
    12301 gtgcagtcgg tttggctttt tctgacgaac aaataagatt cgtggccgac aggtgggggt
    12361 ccaccatgtg aaggcatctt cagactccaa taatggagca atgacgtaag ggcttacgaa
    12421 ataagtaagg gtagtttggg aaatgtccac tcacccgtca gtctataaat acttagcccc
    12481 tccctcattg ttaagggagc aaaatctcag agagatagtc ctagagagag aaagagagca
    12541 agtagcctag aagtagtcaa ggcggcgaag tattcaggca cgtggccagg aagaagaaaa
    12601 gccaagacga cgaaaacagg taagagctaa gcttcctgca ggaacaaagc accagtggtc
    12661 tagtggtaga atagtaccct gccacggtac agacccgggt tcgattcccg gctggtgcag
    12721 tgtatggacg acgatatcag ttttagagct agaaatagca agttaaaata aggctagtcc
    12781 gttatcaact tgaaaaagtg gcaccgagtc ggtgcaacaa agcaccagtg gtctagtggt
    12841 agaatagtac cctgccacgg tacagacccg ggttcgattc ccggctggtg cagtcgatcg
    12901 acaagctcga gtttctccat aataatgtgt gagtagttcc cagataaggg aattagggtt
    12961 cctatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt
    13021 gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc
    13081 cagatccccc gaattagagc tctaccggcg taatatggcg cgccgaagcc aactaaacaa
    13141 gaccataacc atggtgacat ttgacatagt tgtttactac ttgcttgagc cccacccttg
    13201 cttatcggtt gaacattaca agatacactg cgggtggcct aaggcacacc gtccgaaacc
    13261 ggcaaaccaa gcctgatcgc cgaaatccaa aatcactacc ggcaatctct aaagtttatt
    13321 tcatccttat atgacgagga aagaaaagaa gagagaaata atatcttaac ttctaaatca
    13381 gtcgcgtcaa ctttctcggc taagaaagtg agcactatca tttcgaagac catgtcatga
    13441 gtgccgactt gccatatctt attatattct tatttattta attataatcc cattgcaata
    13501 cgtctattct atcatggcct gccactaacg ctccgtctaa cgtcgttaag ccattgtcat
    13561 aagcggctgc tcaaaactct tcccggtgga ggcgaggcgt taacggcgtc tacaaatcta
    13621 acggccacca accatccagc cgcctctcga aagctccgct ccgatcgcgg aaattgcgtg
    13681 gcggagacga gcgggctcct ctcacacggc ccggaaccgt cacggcacgg gtgggggatt
    13741 ccttccccaa ccctccccac ctctcctccc cccgtcgcag cccataaata cagggccctc
    13801 cgcgcctctt cccacaatct cacatcgtct catcgttcgg agcgcacaac ccccgggttc
    13861 caaatccaaa ttgctctcct cgcgaccctc ggcgatcctt cccccgcttc aaggtacggc
    13921 gatcgtctcc cccgtcctct tgccccatct cctcgctcgg cgtggtttgg tggttctgct
    13981 tggtctgtgg ctaggaacta ggctgaggcg ttgacgaaat catgctagat ccgcgtgttt
    14041 cctgatcgtg ggtggctggg aggtggggtt ttcgtgtaga tctgatcggt tccgctgttt
    14101 atcctgtcat gctcatgtga tttgtgggga ttttaggtcg tttgtccggg aatcgtgggg
    14161 ttgcttctag gctgttcgta gatgagatcg ttctcacgat ctgctgggtc gctgcctagg
    14221 ttcagctagg tctgccctgt ttttgggttc gttttcggga tctgtacgtg catctattat
    14281 ctggttcgat ggtgctagct aggaacaaac aactgattcg tccgatcgat tgttttgttg
    14341 ccatgtgcaa ggttaggtcg ttatctgatt gctgtagatc agagtagaat aagatcatca
    14401 caagctagct cttgggctta ttatgaatct gcgtttgttg catgattaag atgattatgc
    14461 tttttcttat gctgccgttt gtatatgatg cggtagcttt taactgaata gcacaccttt
    14521 cctgtttagt tagattagat tagattgcat gatagatgag gatatatgct gctacatcag
    14581 tttgatgatt ctctggtacc tcataatcaa ctagctcatg tgcttaaatt gaaactgcat
    14641 gtgccacatg attaagatgc taagattggt gaagatatat acgctgctgt tcctatagga
    14701 tcctgtagct tttacctggt caacatgcat cgtcctgtta tggatagata tgcatgatag
    14761 atgaagatat gtactgctac aatttgatga ttcttttgtg cacctgatga tcatgcatgc
    14821 tctttgccct tactttgata tacttggatg atggcatgct tagtactaat gatgtgatga
    14881 acacacatga cctgttggta tgaatatgat gttgctgttt gcttgtgatg agttctgttt
    14941 gtttactgct aggcacttac cctgttgtct ggttctcttt tgcagaacct gcaggatgga
    15001 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc
    15061 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
    15121 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg
    15181 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
    15241 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
    15301 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca
    15361 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
    15421 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca
    15481 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
    15541 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac
    15601 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc
    15661 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc
    15721 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt
    15781 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat
    15841 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga
    15901 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga
    15961 agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct
    16021 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt
    16081 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct
    16141 gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt
    16201 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga
    16261 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat
    16321 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc
    16381 caacatcttc gacgctggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc
    16441 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt
    16501 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt
    16561 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc
    16621 caagaagggc ggaaagatcg ccgtgtgacg tcgacgatat gaagatgaag atgaaatatt
    16681 tggtgtgtca aataaaaagc ttgtgtgctt aagtttgtgt ttttttcttg gcttgttgtg
    16741 ttatgaattt gtggcttttt ctaatattaa atgaatgtaa gatcacatta taatgaataa
    16801 acaaatgttt ctataatcca ttgtgaatgt tttgttggat ctcttctgca gcatataact
    16861 actgtatgtg ctatggtatg gactatggaa tatgattaaa gataagccag agctctggtg
    16921 acggacggcg cgactagttt tacgtacgtt aattaacccg gcgagctttg ggtacgtcac
    16981 gtggctcgag gttcgagtat tatggcattg ggaaaactgt ttttcttgta ccatttgttg
    17041 tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat cgaactgtga aatggaaatg
    17101 gatggagaag agttaatgaa tgatatggtc cttttgttca ttctcaaatt aatattattt
    17161 gttttttctc ttatttgttg tgtgttgaat ttgaaattat aagagatatg caaacatttt
    17221 gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat atgaggagta
    17281 aaacacttgt agttgtgagc tcgcgcgtag tcctcggtaa tatcgcagaa caaaagtacc
    17341 tgatatcgag tgtacttcaa gtcacaccgg cgagtgtggc actggccgtc gttttacaac
    17401 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
    17461 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
    17521 gcctgaatgg cgaatgctag agcagcttga gcttggatca gattgtcgtt tcccgccttc
    17581 agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg
    17641 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
    17701 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
    17761 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
    17821 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
    17881 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
    17941 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
    18001 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
    18061 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
    18121 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
    18181 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
    18241 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
    18301 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
    18361 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
    18421 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
    18481 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
    18541 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
    18601 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
    18661 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
    18721 gcacggctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
    18781 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
    18841 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
    18901 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
    18961 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
    19021 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
    19081 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
    19141 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
    19201 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
    19261 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
    19321 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
    19381 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
    19441 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
    19501 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
    19561 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
    19621 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
    19681 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
    19741 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
    19801 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
    19861 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
    19921 agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
    19981 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
    20041 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
    20101 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
    20161 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
    20221 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
    20281 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
    20341 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
    20401 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
    20461 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
    20521 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
    20581 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
    20641 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
    20701 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
    20761 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
    20821 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
    20881 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
    20941 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
    21001 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
    21061 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggcctctttc
    21121 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
    21181 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
    21241 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
    21301 gcctggcctg tgcataactg tctggccagc gcacagccca agagctgcaa aaagcgccta
    21361 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
    21421 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
    21481 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
    21541 gacggtgaaa acctctgaca catgcagctc ccggaaacgg tcacagcttg tctgtaagcg
    21601 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
    21661 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
    21721 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
    21781 ggagaaaata ccgcatcagg ccctcttccg cttcctcgct cactgactcg ctgcgctcgg
    21841 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    21901 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    21961 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    22021 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    22081 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    22141 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    22201 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    22261 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    22321 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
//
