LOCUS       sequence-367250-       22716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA for creating transgenic plants expressing Cas9 and Drm1b 
            gRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22716)
  AUTHORS   Weiss T, Wang C, Kang X, Zhao H, Elena Gamo M, Starker CG, Crisp PA,
            Zhou P, Springer NM, Voytas DF, Zhang F
  TITLE     Optimization of multiplexed CRISPR/Cas9 system for highly efficient 
            genome editing in Setaria viridis.
  JOURNAL   Plant J. 2020 Nov;104(3):828-838. doi: 10.1111/tpj.14949. Epub 2020 
            Sep 8.
  PUBMED    32786122
REFERENCE   2  (bases 1 to 22716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.14949";
            journalName: "Plant J"; date: "2020-11"; volume: "104"; issue: "3"; 
            pages: "828-838"
COMMENT     Sequence Label: pTW045
FEATURES             Location/Qualifiers
     source          1..22716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1827..2852)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    4792..4813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4828..4833,4834..4851,4852..4858)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4866..4882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4871..4893
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4890..4906
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4890..4906
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7671..7727
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7728..11831
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7728..11831
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11832..11879
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_RNA        12740..12815
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        12913..12988
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        13086..13161
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     polyA_signal    13258..13432
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             15342..16994
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(15408..15426)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      17004..17252
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(17727..17744)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(17727..17743)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17736..17758)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    17946..17970
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             19271..19900
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             20329..21402
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      21468..21662
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(21898..21920)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    22006..22146
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     22061..22080
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(22161..22178)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(22332..22716,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(22412..22431)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgacagatc ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt
     1861 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg
     1921 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
     1981 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca
     2041 agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc
     2101 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg
     2161 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt
     2221 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg
     2281 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca
     2341 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat
     2401 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg
     2461 ctcgtctggc taagatcggc cgcagcgatc gcatccatag cctccgcgac cggttgtaga
     2521 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtga acggcgggag
     2581 atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc
     2641 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta
     2701 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
     2761 ccctccgaga gctgcatcag gtcggacacg ctgtcgaact tttcgatcag aaacttctcg
     2821 acagacgtcg cggtgagttc aggctttttc ataagcttct gcaaaagaga accagacaac
     2881 agggtaagtg cctagcagta aacaaacaga actcatcaca agcaaacagc aacatcatat
     2941 tcataccaac aggtcatgtg tgttcatcac atcattagta ctaagcatgc catcatccaa
     3001 gtatatcaaa gtaagggcaa agagcatgca tgatcatcag gtgcacaaaa gaatcatcaa
     3061 attgtagcag tacatatctt catctatcat gcatatctat ccataacagg acgatgcatg
     3121 ttgaccaggt aaaagctaca ggatcctata ggaacagcag cgtatatatc ttcaccaatc
     3181 ttagcatctt aatcatgtgg cacatgcagt ttcaatttaa gcacatgagc tagttgatta
     3241 tgaggtacca gagaatcatc aaactgatgt agcagcatat atcctcatct atcatgcaat
     3301 ctaatctaat ctaactaaac aggaaaggtg tgctattcag ttaaaagcta ccgcatcata
     3361 tacaaacggc agcataagaa aaagcataat catcttaatc atgcaacaaa cgcagattca
     3421 taataagccc aagagctagc ttgtgatgat cttattctac tctgatctac agcaatcaga
     3481 taacgaccta accttgcaca tggcaacaaa acaatcgatc ggacgaatca gttgtttgtt
     3541 cctagctagc accatcgaac cagataatag atgcacgtac agatcccgaa aacgaaccca
     3601 aaaacagggc agacctagct gaacctaggc agcgacccag cagatcgtga gaacgatctc
     3661 atctacgaac agcctagaag caaccccacg attcccggac aaacgaccta aaatccccac
     3721 aaatcacatg agcatgacag gataaacagc ggaaccgatc agatctacac gaaaacccca
     3781 cctcccagcc acccacgatc aggaaacacg cggatctagc atgatttcgt caacgcctca
     3841 gcctagttcc tagccacaga ccaagcagaa ccaccaaacc acgccgagcg aggagatggg
     3901 gcaagaggac gggggagacg atcgccgtac cttgaagcgg gggaaggatc gccgagggtc
     3961 gcgaggagag caatttggat ttggaacccg ggggttgtgc gctccgaacg atgagacgat
     4021 gtgagattgt gggaagaggc gcggagggcc ctgtatttat gggctgcgac ggggggagga
     4081 gaggtgggga gggttgggga aggaatcccc cacccgtgcc gtgacggttc cgggccgtgt
     4141 gagaggagcc cgctcgtctc cgccacgcaa tttccgcgat cggagcggag ctttcgagag
     4201 gcggctggat ggttggtggc cgttagattt gtagacgccg ttaacgcctc gcctccaccg
     4261 ggaagagttt tgagcagccg cttatgacaa tggcttaacg acgttagacg gagcgttagt
     4321 ggcaggccat gatagaatag acgtattgca atgggattat aattaaataa ataagaatat
     4381 aataagatat ggcaagtcgg cactcatgac atggtcttcg aaatgatagt gctcactttc
     4441 ttagccgaga aagttgacgc gactgattta gaagttaaga tattatttct ctcttctttt
     4501 ctttcctcgt catataagga tgaaataaac tttagagatt gccggtagtg attttggatt
     4561 tcggcgatca ggcttggttt gccggtttcg gacggtgtgc cttaggccac ccgcagtgta
     4621 tcttgtaatg ttcaaccgat aagcaagggt ggggctcaag caagtagtaa acaactatgt
     4681 caaatgtcac catggttatg gtcttgttta gttggcttct tggccgattc attaatgcag
     4741 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     4801 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     4861 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc
     4921 gagctcggta cccggggatc tgcaggcgcg cctgcagtgc agcgtgaccc ggtcgtgccc
     4981 ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt
     5041 tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa actttactct
     5101 acgaataata taatctatag tactacaata atatcagtgt tttagagaat catataaatg
     5161 aacagttaga catggtctaa aggacaattg agtattttga caacaggact ctacagtttt
     5221 atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac
     5281 ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa
     5341 tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct
     5401 attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa gtgactaaaa
     5461 attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag
     5521 tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc
     5581 agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg
     5641 acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt
     5701 gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc
     5761 accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc
     5821 gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt tcggagcgca
     5881 cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt caaggtacgc
     5941 cgctcgtcct cccccccccc cctctctacc ttctctagat cggcgttccg gtccatggtt
     6001 agggcccggt agttctactt ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc
     6061 gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca gacacgttct gattgctaac
     6121 ttgccagtgt ttctctttgg ggaatcctgg gatggctcta gccgttccgc agacgggatc
     6181 gatttcatga ttttttttgt ttcgttgcat agggtttggt ttgccctttt cctttatttc
     6241 aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat gctttttttt gtcttggttg
     6301 tgatgatgtg gtctggttgg gcggtcgttc tagatcggag tagaattaat tctgtttcaa
     6361 actacctggt ggatttatta attttggatc tgtatgtgtg tgccatacat attcatagtt
     6421 acgaattgaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg
     6481 ttttactgat gcatatacag agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg
     6541 ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgt
     6601 atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac gagtttaaga
     6661 tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata
     6721 tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc tatctattat
     6781 aataaacaag tatgttttat aattattttg atcttgatat acttggatga tggcatatgc
     6841 agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt gcttggtact
     6901 gtttcttttg tcgatgctca ccctgttgtt tggtgttact tcctgcagga agcatgtctg
     6961 agccacctcg ggctgagacc tttgtattcc tggacctaga agccactggg ctcccaaaca
     7021 tggaccctga gattgcagag atatcccttt ttgctgttca ccgctcttcc ctggagaacc
     7081 cagaacggga tgattctggt tccttggtgc tgccccgtgt tctggacaag ctcacactgt
     7141 gcatgtgccc ggagcgcccc tttactgcca aggccagtga gattactggt ttgagcagcg
     7201 aaagcctgat gcactgcggg aaggctggtt tcaatggcgc tgtggtaagg acactgcagg
     7261 gcttcctaag ccgccaggag ggccccatct gccttgtggc ccacaatggc ttcgattatg
     7321 acttcccact gctgtgcacg gagctacaac gtctgggtgc ccatctgccc caagacactg
     7381 tctgcctgga cacactgcct gcattgcggg gcctggaccg tgctcacagc cacggcacca
     7441 gggctcaagg ccgcaaaagc tacagcctgg ccagtctctt ccaccgctac ttccaggctg
     7501 aacccagtgc tgcccattca gcagaaggtg atgtgcacac cctgcttctg atcttcctgc
     7561 atcgtgctcc tgagctgctc gcctgggcag atgagcaggc ccgcagctgg gctcatattg
     7621 agcccatgta cgtgccacct gatggtccaa gcctcgaagc cggctccggc gccaccaact
     7681 tctccctcct caagcaagcc ggcgacgtgg aggagaaccc aggcccaatg gacaagaagt
     7741 actcgatcgg cctcgacatc gggacgaact cagttggctg ggccgtgatc accgacgagt
     7801 acaaggtgcc ctctaagaag ttcaaggtcc tggggaacac cgaccgccat tccatcaaga
     7861 agaacctcat cggcgctctc ctgttcgaca gcggggagac cgctgaggct acgaggctca
     7921 agagaaccgc taggcgccgg tacacgagaa ggaagaacag gatctgctac ctccaagaga
     7981 ttttctccaa cgagatggcc aaggttgacg attcattctt ccaccgcctg gaggagtctt
     8041 tcctcgtgga ggaggataag aagcacgagc ggcatcccat cttcggcaac atcgtggacg
     8101 aggttgccta ccacgagaag taccctacga tctaccatct gcggaagaag ctcgtggact
     8161 ccaccgataa ggcggacctc agactgatct acctcgctct ggcccacatg atcaagttcc
     8221 gcggccattt cctgatcgag ggggatctca acccagacaa cagcgatgtt gacaagctgt
     8281 tcatccaact cgtgcagacc tacaaccaac tcttcgagga gaacccgatc aacgcctctg
     8341 gcgtggacgc gaaggctatc ctgtccgcga ggctctcgaa gtccaggagg ctggagaacc
     8401 tgatcgctca gctcccaggc gagaagaaga acggcctgtt cgggaacctc atcgctctca
     8461 gcctggggct caccccgaac ttcaagtcga acttcgatct cgctgaggac gccaagctgc
     8521 aactctccaa ggacacctac gacgatgacc tcgataacct cctggcccag atcggcgatc
     8581 aatacgcgga cctgttcctc gctgccaaga acctgtcgga cgccatcctc ctgtcagata
     8641 tcctccgcgt gaacaccgag atcacgaagg ctccactctc tgcctccatg atcaagcgct
     8701 acgacgagca ccatcaggat ctgaccctcc tgaaggcgct ggtccgccaa cagctcccgg
     8761 agaagtacaa ggagattttc ttcgatcagt cgaagaacgg ctacgctggg tacatcgacg
     8821 gcggggcctc acaagaggag ttctacaagt tcatcaagcc aatcctggag aagatggacg
     8881 gcacggagga gctcctggtg aagctcaaca gggaggacct cctgcggaag cagagaacct
     8941 tcgataacgg cagcatcccc caccaaatcc atctcgggga gctgcacgcc atcctgagaa
     9001 ggcaagagga cttctaccct ttcctcaagg ataaccggga gaagatcgag aagatcctga
     9061 ccttcagaat cccatactac gtcggccctc tcgcgcgggg gaactcaaga ttcgcttgga
     9121 tgacccgcaa gtctgaggag accatcacgc cgtggaactt cgaggaggtg gtggacaagg
     9181 gcgctagcgc tcagtcgttc atcgagagga tgaccaactt cgacaagaac ctgcccaacg
     9241 agaaggtgct ccctaagcac tcgctcctgt acgagtactt caccgtctac aacgagctca
     9301 cgaaggtgaa gtacgtcacc gagggcatgc gcaagccagc gttcctgtcc ggggagcaga
     9361 agaaggctat cgtggacctc ctgttcaaga ccaaccggaa ggtcacggtt aagcaactca
     9421 aggaggacta cttcaagaag atcgagtgct tcgattcggt cgagatcagc ggcgttgagg
     9481 accgcttcaa cgccagcctc gggacctacc acgatctcct gaagatcatc aaggataagg
     9541 acttcctgga caacgaggag aacgaggata tcctggagga catcgtgctg accctcacgc
     9601 tgttcgagga cagggagatg atcgaggagc gcctgaagac gtacgcccat ctcttcgatg
     9661 acaaggtcat gaagcaactc aagcgccgga gatacaccgg ctgggggagg ctgtcccgca
     9721 agctcatcaa cggcatccgg gacaagcagt ccgggaagac catcctcgac ttcctcaaga
     9781 gcgatggctt cgccaacagg aacttcatgc aactgatcca cgatgacagc ctcaccttca
     9841 aggaggatat ccaaaaggct caagtgagcg gccaggggga ctcgctgcac gagcatatcg
     9901 cgaacctcgc tggctccccc gcgatcaaga agggcatcct ccagaccgtg aaggttgtgg
     9961 acgagctcgt gaaggtcatg ggccggcaca agcctgagaa catcgtcatc gagatggcca
    10021 gagagaacca aaccacgcag aaggggcaaa agaactctag ggagcgcatg aagcgcatcg
    10081 aggagggcat caaggagctg gggtcccaaa tcctcaagga gcacccagtg gagaacaccc
    10141 aactgcagaa cgagaagctc tacctgtact acctccagaa cggcagggat atgtacgtgg
    10201 accaagagct ggatatcaac cgcctcagcg attacgacgt cgatcatatc gttccccagt
    10261 ctttcctgaa ggatgactcc atcgacaaca aggtcctcac caggtcggac aagaaccgcg
    10321 gcaagtcaga taacgttcca tctgaggagg tcgttaagaa gatgaagaac tactggaggc
    10381 agctcctgaa cgccaagctg atcacgcaaa ggaagttcga caacctcacc aaggctgaga
    10441 gaggcgggct ctcagagctg gacaaggccg gcttcatcaa gcggcagctg gtcgagacca
    10501 gacaaatcac gaagcacgtt gcgcaaatcc tcgactctcg gatgaacacg aagtacgatg
    10561 agaacgacaa gctgatcagg gaggttaagg tgatcaccct gaagtctaag ctcgtctccg
    10621 acttcaggaa ggatttccag ttctacaagg ttcgcgagat caacaactac caccatgccc
    10681 atgacgctta cctcaacgct gtggtcggca ccgctctgat caagaagtac ccaaagctgg
    10741 agtccgagtt cgtgtacggg gactacaagg tttacgatgt gcgcaagatg atcgccaagt
    10801 cggagcaaga gatcggcaag gctaccgcca agtacttctt ctactcaaac atcatgaact
    10861 tcttcaagac cgagatcacg ctggccaacg gcgagatccg gaagagaccg ctcatcgaga
    10921 ccaacggcga gacgggggag atcgtgtggg acaagggcag ggatttcgcg accgtccgca
    10981 aggttctctc catgccccag gtgaacatcg tcaagaagac cgaggtccaa acgggcgggt
    11041 tctcaaagga gtctatcctg cctaagcgga acagcgacaa gctcatcgcc agaaagaagg
    11101 actgggaccc aaagaagtac ggcgggttcg acagccctac cgtggcctac tcggtcctgg
    11161 ttgtggcgaa ggttgagaag ggcaagtcca agaagctcaa gagcgtgaag gagctcctgg
    11221 ggatcaccat catggagagg tccagcttcg agaagaaccc aatcgacttc ctggaggcca
    11281 agggctacaa ggaggtgaag aaggacctga tcatcaagct cccgaagtac tctctcttcg
    11341 agctggagaa cggcaggaag agaatgctgg cttccgctgg cgagctccag aaggggaacg
    11401 agctcgcgct gccaagcaag tacgtgaact tcctctacct ggcttcccac tacgagaagc
    11461 tcaagggcag cccggaggac aacgagcaaa agcagctgtt cgtcgagcag cacaagcatt
    11521 acctcgacga gatcatcgag caaatctccg agttcagcaa gcgcgtgatc ctcgccgacg
    11581 cgaacctgga taaggtcctc tccgcctaca acaagcaccg ggacaagccc atcagagagc
    11641 aagcggagaa catcatccat ctcttcaccc tgacgaacct cggcgctcct gctgctttca
    11701 agtacttcga caccacgatc gatcggaaga gatacacctc cacgaaggag gtcctggacg
    11761 cgaccctcat ccaccagtcg atcaccggcc tgtacgagac gaggatcgac ctctcacaac
    11821 tcggcgggga taagagaccc gcagcaacca agaaggcagg gcaagcaaag aagaagaagt
    11881 gactcgagta tatgaagatg aatatgaaat atttggtgtg tcaaataaaa agcttgtgtg
    11941 cttaagtttg tgtttttttc ttggcttgtt gtgttatgaa tttgtggctt tttctaatat
    12001 taaatgaatg taagatcaca ttataatgaa taaacaaatg tttctataat ccattgtgaa
    12061 tgttttgttg gatctcttct gcagcatata actactgtat gtgctatggt atggactatg
    12121 gaatatgatt aaagataagg agctcgttaa ccggtgacgg acggcgcgct ggcagacata
    12181 ctgtcccaca aatgaagatg gaatctgtaa aagaaaacgc gtgaaataat gcgtctgaca
    12241 aaggttaggt cggctgcctt taatcaatac caaagtggtc cctaccacga tggaaaaact
    12301 gtgcagtcgg tttggctttt tctgacgaac aaataagatt cgtggccgac aggtgggggt
    12361 ccaccatgtg aaggcatctt cagactccaa taatggagca atgacgtaag ggcttacgaa
    12421 ataagtaagg gtagtttggg aaatgtccac tcacccgtca gtctataaat acttagcccc
    12481 tccctcattg ttaagggagc aaaatctcag agagatagtc ctagagagag aaagagagca
    12541 agtagcctag aagtagtcaa ggcggcgaag tattcaggca cgtggccagg aagaagaaaa
    12601 gccaagacga cgaaaacagg taagagctaa gcttcctgca ggaacaaagc accagtggtc
    12661 tagtggtaga atagtaccct gccacggtac agacccgggt tcgattcccg gctggtgcat
    12721 tgccgtcttg ggtcccacgg ttttagagct agaaatagca agttaaaata aggctagtcc
    12781 gttatcaact tgaaaaagtg gcaccgagtc ggtgcaacaa agcaccagtg gtctagtggt
    12841 agaatagtac cctgccacgg tacagacccg ggttcgattc ccggctggtg cagtgtatgg
    12901 acgacgatat cagttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
    12961 acttgaaaaa gtggcaccga gtcggtgcaa caaagcacca gtggtctagt ggtagaatag
    13021 taccctgcca cggtacagac ccgggttcga ttcccggctg gtgcaaatgc ctctcccaaa
    13081 cccaagtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
    13141 aaagtggcac cgagtcggtg caacaaagca ccagtggtct agtggtagaa tagtaccctg
    13201 ccacggtaca gacccgggtt cgattcccgg ctggtgcagt cgatcgacaa gctcgagttt
    13261 ctccataata atgtgtgagt agttcccaga taagggaatt agggttccta tagggtttcg
    13321 ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta
    13381 tcaataaaat ttctaattcc taaaaccaaa atccagtact aaaatccaga tcccccgaat
    13441 tagagctcta ccggcgtaat atggcgcgcc gaagccaact aaacaagacc ataaccatgg
    13501 tgacatttga catagttgtt tactacttgc ttgagcccca cccttgctta tcggttgaac
    13561 attacaagat acactgcggg tggcctaagg cacaccgtcc gaaaccggca aaccaagcct
    13621 gatcgccgaa atccaaaatc actaccggca atctctaaag tttatttcat ccttatatga
    13681 cgaggaaaga aaagaagaga gaaataatat cttaacttct aaatcagtcg cgtcaacttt
    13741 ctcggctaag aaagtgagca ctatcatttc gaagaccatg tcatgagtgc cgacttgcca
    13801 tatcttatta tattcttatt tatttaatta taatcccatt gcaatacgtc tattctatca
    13861 tggcctgcca ctaacgctcc gtctaacgtc gttaagccat tgtcataagc ggctgctcaa
    13921 aactcttccc ggtggaggcg aggcgttaac ggcgtctaca aatctaacgg ccaccaacca
    13981 tccagccgcc tctcgaaagc tccgctccga tcgcggaaat tgcgtggcgg agacgagcgg
    14041 gctcctctca cacggcccgg aaccgtcacg gcacgggtgg gggattcctt ccccaaccct
    14101 ccccacctct cctccccccg tcgcagccca taaatacagg gccctccgcg cctcttccca
    14161 caatctcaca tcgtctcatc gttcggagcg cacaaccccc gggttccaaa tccaaattgc
    14221 tctcctcgcg accctcggcg atccttcccc cgcttcaagg tacggcgatc gtctcccccg
    14281 tcctcttgcc ccatctcctc gctcggcgtg gtttggtggt tctgcttggt ctgtggctag
    14341 gaactaggct gaggcgttga cgaaatcatg ctagatccgc gtgtttcctg atcgtgggtg
    14401 gctgggaggt ggggttttcg tgtagatctg atcggttccg ctgtttatcc tgtcatgctc
    14461 atgtgatttg tggggatttt aggtcgtttg tccgggaatc gtggggttgc ttctaggctg
    14521 ttcgtagatg agatcgttct cacgatctgc tgggtcgctg cctaggttca gctaggtctg
    14581 ccctgttttt gggttcgttt tcgggatctg tacgtgcatc tattatctgg ttcgatggtg
    14641 ctagctagga acaaacaact gattcgtccg atcgattgtt ttgttgccat gtgcaaggtt
    14701 aggtcgttat ctgattgctg tagatcagag tagaataaga tcatcacaag ctagctcttg
    14761 ggcttattat gaatctgcgt ttgttgcatg attaagatga ttatgctttt tcttatgctg
    14821 ccgtttgtat atgatgcggt agcttttaac tgaatagcac acctttcctg tttagttaga
    14881 ttagattaga ttgcatgata gatgaggata tatgctgcta catcagtttg atgattctct
    14941 ggtacctcat aatcaactag ctcatgtgct taaattgaaa ctgcatgtgc cacatgatta
    15001 agatgctaag attggtgaag atatatacgc tgctgttcct ataggatcct gtagctttta
    15061 cctggtcaac atgcatcgtc ctgttatgga tagatatgca tgatagatga agatatgtac
    15121 tgctacaatt tgatgattct tttgtgcacc tgatgatcat gcatgctctt tgcccttact
    15181 ttgatatact tggatgatgg catgcttagt actaatgatg tgatgaacac acatgacctg
    15241 ttggtatgaa tatgatgttg ctgtttgctt gtgatgagtt ctgtttgttt actgctaggc
    15301 acttaccctg ttgtctggtt ctcttttgca gaacctgcag gatggaagac gccaaaaaca
    15361 taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc
    15421 ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata
    15481 tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta
    15541 tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc
    15601 aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg
    15661 acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt
    15721 tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc
    15781 aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt
    15841 tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg
    15901 atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag
    15961 gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt
    16021 ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt
    16081 ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata
    16141 gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc
    16201 tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat
    16261 ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg
    16321 ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat
    16381 cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc
    16441 cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa
    16501 gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag
    16561 cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg
    16621 acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct
    16681 atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg
    16741 ctggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt
    16801 tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa
    16861 caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta
    16921 ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa
    16981 agatcgccgt gtgacgtcga cgatatgaag atgaagatga aatatttggt gtgtcaaata
    17041 aaaagcttgt gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg
    17101 ctttttctaa tattaaatga atgtaagatc acattataat gaataaacaa atgtttctat
    17161 aatccattgt gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat
    17221 ggtatggact atggaatatg attaaagata agccagagct ctggtgacgg acggcgcgac
    17281 tagttttacg tacgttaatt aacccggcga gctttgggta cgtcacgtgg ctcgaggttc
    17341 gagtattatg gcattgggaa aactgttttt cttgtaccat ttgttgtgct tgtaatttac
    17401 tgtgtttttt attcggtttt cgctatcgaa ctgtgaaatg gaaatggatg gagaagagtt
    17461 aatgaatgat atggtccttt tgttcattct caaattaata ttatttgttt tttctcttat
    17521 ttgttgtgtg ttgaatttga aattataaga gatatgcaaa cattttgttt tgagtaaaaa
    17581 tgtgtcaaat cgtggcctct aatgaccgaa gttaatatga ggagtaaaac acttgtagtt
    17641 gtgagctcgc gcgtagtcct cggtaatatc gcagaacaaa agtacctgat atcgagtgta
    17701 cttcaagtca caccggcgag tgtggcactg gccgtcgttt tacaacgtcg tgactgggaa
    17761 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    17821 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
    17881 tgctagagca gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca
    17941 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat
    18001 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
    18061 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca
    18121 gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa
    18181 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
    18241 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
    18301 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
    18361 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
    18421 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
    18481 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
    18541 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
    18601 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
    18661 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
    18721 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
    18781 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
    18841 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
    18901 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
    18961 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
    19021 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac ggctcaaccg
    19081 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
    19141 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
    19201 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca
    19261 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
    19321 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
    19381 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
    19441 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
    19501 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
    19561 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
    19621 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
    19681 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
    19741 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
    19801 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
    19861 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
    19921 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
    19981 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
    20041 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
    20101 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
    20161 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
    20221 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc
    20281 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc
    20341 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg
    20401 aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg
    20461 tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg
    20521 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc
    20581 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg
    20641 aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag
    20701 gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg
    20761 gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc
    20821 cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga
    20881 aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg
    20941 cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg
    21001 attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag
    21061 ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt
    21121 caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc
    21181 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc
    21241 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac
    21301 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc
    21361 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta
    21421 gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggcc tctttcctgt ggatagcacg
    21481 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag
    21541 ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat
    21601 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca
    21661 taactgtctg gccagcgcac agcccaagag ctgcaaaaag cgcctaccct tcggtcgctg
    21721 cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg
    21781 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac
    21841 cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
    21901 ctgacacatg cagctcccgg aaacggtcac agcttgtctg taagcggatg ccgggagcag
    21961 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
    22021 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
    22081 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
    22141 atcaggccct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    22201 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    22261 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    22321 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    22381 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    22441 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    22501 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    22561 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    22621 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    22681 gcagccactg gtaacaggat tagcagagcg aggtat
//
