LOCUS       sequence-36726-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Emdadul Haque M, Grasso D, Miller C, Spremulli LL, Saada A
  TITLE     The effect of mutated mitochondrial ribosomal proteins S16 and S22 
            on the assembly of the small and large ribosomal subunits in human 
            mitochondria.
  JOURNAL   Mitochondrion. 2008 Jun . 8(3):254-61.
  PUBMED    18539099
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Mitochondrion. 2008 Jun . 8(3):254-61."
COMMENT     Sequence Label: pET-MRPL15
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             799..816
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(869..887)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgtg tggcagaggc
       61 cataaaggag aaaggcaaag aggaacccgg ccccgcttgg gctttgaggg aggccagact
      121 ccattttaca tccgaatccc aaaatacggg tttaacgaag gacatagttt cagacgccag
      181 tataagcctt tgagtctcaa tagactgcag tatcttattg atttgggtcg tgttgatcct
      241 agtcaaccta ttgacttaac ccagcttgtc aatgggagag gtgtgaccat ccagccactt
      301 aaaagggatt atggtgtcca gctggttgag gagggtgctg acacctttac ggcaaaagtt
      361 aatattgaag tacagttggc ttcagaacta gctattgctg ccattgaaaa aaatggtggt
      421 gttgttacta cagccttcta tgatccaaga agtctggaca ttgtatgcaa acctgttcca
      481 ttctttcttc gtggacaacc cattccaaaa agaatgcttc caccagaaga actggtacca
      541 tattacactg atgcaaagaa ccgtgggtac ctggcggatc ctgccaaatt tcctgaagca
      601 cgacttgaac tcgccaggaa gtatggttat atcttacctg atatcactaa agatgaactc
      661 ttcaaaatgc tctgtactag gaaggatcca aggcagattt tctttggtct tgctccagga
      721 tgggtggtga atatggccga taagaaaatc ctaaaaccta cagatgaaaa tctccttaag
      781 tattatacct cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc
      841 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
      901 gcctctaaac gggtcttgag gg
//
