LOCUS       sequence-367267-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GC6
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(74..662)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(154..173)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1070..1086
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1070..1086
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1091..1110
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1091..1109
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1284..2474)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2103..2122
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(2522..2527,2528..2544,2545..2550))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
       61 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      121 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      181 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      241 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      301 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      361 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      421 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      481 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      541 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      601 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      661 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgac gcgtaactca
      721 cgttaaggga ttttggtcat gggtggctcg accagatatg gtctcggatt gctagtaaca
      781 atggtggcaa acaggctctt gaaaccgtac agcgcctact gccagttctc tgtcaagccc
      841 acggtctgac tccggagcaa gttgtagcga ttgctagtca tgacggtggc aaacaggctc
      901 ttgaaaccgt ccaacgcctt ctaccagttc tctgtcaagc ccacggacta accccagcgc
      961 aagttgtagc gatagcctga gaccagcttt ggtctagagg aggcatgatg atatattttt
     1021 atcttgtgca atgtaacatc agagattttg agacacgggc cagagctgcc aggaaacagc
     1081 tatgaccatg taatacgact cactataggg gatattttat tttctcccaa tcaggcttga
     1141 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatcaag ggttggtttg
     1201 cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt
     1261 agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc
     1321 aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat
     1381 gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccaatgat cgaagttagg
     1441 ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg
     1501 gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg
     1561 gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc
     1621 gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg
     1681 aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc
     1741 gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct
     1801 acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc
     1861 caccggaagg agctgactgg gttgaaggct ctcaagggca tgggtcggcg ctctccctta
     1921 tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc
     1981 gcaaggaatg gtgcatgtaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
     2041 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
     2101 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
     2161 acgatgcgtc cggcgtagag aatccacagg acgggtgtgg tcgccatgat cgcgtagtcg
     2221 atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt
     2281 gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca
     2341 tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc
     2401 ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca
     2461 ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg
     2521 cattaaagct aatcgatgat aagctgtcaa acatgagaat taattcatgt tctttcctgc
     2581 gttatcccct gattctgtgg ata
//
