LOCUS       sequence-36727-e         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Emdadul Haque M, Grasso D, Miller C, Spremulli LL, Saada A
  TITLE     The effect of mutated mitochondrial ribosomal proteins S16 and S22 
            on the assembly of the small and large ribosomal subunits in human 
            mitochondria.
  JOURNAL   Mitochondrion. 2008 Jun . 8(3):254-61.
  PUBMED    18539099
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Mitochondrion. 2008 Jun . 8(3):254-61."
COMMENT     Sequence Label: pET21-MRPL13
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             472..489
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(542..560)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      556..603
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(727..746)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     937..958
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgca gggattacat
       61 aaacctgtgt accatgcact gagtgactgt ggggatcatg ttgttataat gaacacaaga
      121 cacattgcat tttctggaaa caaatgggaa caaaaagtat actcttcgca tactggctac
      181 ccaggtggat ttagacaagt aacagctgct cagcttcacc tgagggatcc agtggcaatt
      241 gtaaaactag ctatttatgg catgctgcca aaaaaccttc acagaagaac aatgatggaa
      301 aggttgcatc tttttccaga tgagtatatt ccagaagata ttcttaagaa tttagtagag
      361 gagcttcctc aaccacgaaa aatacctaaa cgtctagatg agtacacaca agaagaaata
      421 gacgccttcc caagattgtg gactccacct gaagattatc ggctactcga gcaccaccac
      481 caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
      541 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      601 ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat
      661 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      721 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      781 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      841 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
      901 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
      961 cacactcaac cctatctcgg tctatc
//
