LOCUS       sequence-36728-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3619 (=HL 716 + pHL 1228)
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    156..172
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        230..334
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(553..572)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgggaagtct tctccttncg catgctagct gggaccagga gggacagtac tttaactttc
       61 atgttattaa ccctctgtta tatgccttta tttgcttttt tatgccactg catactgatt
      121 aaccctcatt aatcagtcgg caagtcgaca gtatcttgtt atccgctcac aatgtcaatg
      181 ttatccgctc acatttatga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      241 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      301 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
      361 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
      421 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
      481 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
      541 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
      601 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
      661 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
      721 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
      781 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg acgatgagct
      841 a
//
