LOCUS       sequence-36729-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3612 (=HL 716 + pHL 841)
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    146..162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        220..324
                     /gene="bla"
                     /label=AmpR promoter
     CDS             509..887
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(543..562)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tctcctttcg catgctagct gggaccagga gggacagtac tttaactttc atgttattaa
       61 ccctctgtta tatgccttta tttgcttttt tatgccactg catactgatt aaccctcatt
      121 aatcagtcgg caagtcgaca gtatcttgtt atccgctcac aatgtcaatg ttatccgctc
      181 acatttatga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      241 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      301 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      361 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      421 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      481 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      541 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
      601 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      661 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      721 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      781 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
      841 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
//
