LOCUS       sequence-367300-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AA9
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     367..383
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     367..383
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     388..407
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        388..406
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(581..1771)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1400..1419
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1819..1824,1825..1841,1842..1847))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(1974..2562)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2054..2073)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtggaacgac gcgtaactca cgttaaggga ttttggtcat gggtggctcg accagatatg
       61 gtctcaagag gcaatcgcta caacttgatc tggggtgagt ccgtgggctt gacagagaac
      121 tggaaggagg cgctgaaccg tctcaagagc ctgtttgcca ccaatattac tagcaatcgc
      181 tacaacttga tctggggtga gtccgtgggc ttgacagaga actggaagga ggcgctgaac
      241 cgtctcaaga gcctgtttgc caccaatatt ggaacgagac cagctttggt ctagaggagg
      301 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacgggccag
      361 agctgccagg aaacagctat gaccatgtaa tacgactcac tataggggat attttatttt
      421 ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc
      481 gatcaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt
      541 ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc
      601 tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc
      661 atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc
      721 caatgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt
      781 cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag
      841 cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt
      901 agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg
      961 tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg
     1021 acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg
     1081 ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga
     1141 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatgg
     1201 gtcggcgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
     1261 cgttgagcac cgccgccgca aggaatggtg catgtaagga gatggcgccc aacagtcccc
     1321 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     1381 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     1441 cgccggtgat gccggccacg atgcgtccgg cgtagagaat ccacaggacg ggtgtggtcg
     1501 ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc
     1561 caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata
     1621 gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga
     1681 ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga
     1741 ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta
     1801 actgtgataa actaccgcat taaagctaat cgatgataag ctgtcaaaca tgagaattaa
     1861 ttcatgttct ttcctgcgtt atcccctgat tctgtggata acgcaggaaa gaacatgtga
     1921 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1981 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2041 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2101 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2161 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2221 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2281 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     2341 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2401 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     2461 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     2521 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     2581 tctacggggt ctgacgctca
//
