LOCUS       sequence-367330-        9896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For the cloning of arRNA-expressing vectors containing BFP 
            fluorescence marker in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Qu L, Yi Z, Zhu S, Wang C, Cao Z, Zhou Z, Yuan P, Yu Y, Tian F, Liu 
            Z, Bao Y, Zhao Y, Wei W
  TITLE     Programmable RNA editing by recruiting endogenous ADAR using 
            engineered RNAs.
  JOURNAL   Nat Biotechnol. 2019 Sep;37(9):1059-1069. doi: 
            10.1038/s41587-019-0178-z. Epub 2019 Jul 15.
  PUBMED    31308540
REFERENCE   2  (bases 1 to 9896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-019-0178-z"; journalName: "Nat Biotechnol"; date: 
            "2019-09"; volume: "37"; issue: "9"; pages: "1059-1069"
COMMENT     Sequence Label: plenti-arRNA-BFP-backbone
FEATURES             Location/Qualifiers
     source          1..9896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2641..2889
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2641..2661
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2812..2831
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     2913..2932
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2913..2931
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2961..3085
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(3514..3819)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3616..3635)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(4139..4822)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     4737..4756
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4823..4925)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(5103..5227)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     5279..5295
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5337..5370)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        5486..5789
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5790..5993
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             6028..6729
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    6785..7373
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6838..6858)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7256..7267)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7376..7392)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7902..8082
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8144..8732)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8224..8243)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8903..9694,9695..9763))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9526..9545
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9764..9868)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgctctagag gtaccaaggt ctggcaggaa
     2641 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
     2701 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
     2761 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
     2821 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     2881 cgaaacaccg agagacgctg gcttatcgaa attaatacga ctcactatag ggagacccaa
     2941 gctggctagt taagctatca acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg
     3001 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
     3061 aaacacaaca tatccagtca ctatgaatca actacttaga tggtattagt gacctgtagt
     3121 cgaccgacag ccttccaaat gttcttcggg tgatgctgcc aacttagtcg accgacagcc
     3181 ttccaaatgt tcttctcaaa cggaatcgtc gtatccagcc tactcgctat tgtcctcaat
     3241 gccgtattaa atcataaaaa gaaataagaa aaagaggtgc gagcctcttt tttgtgtgac
     3301 aaaataaaaa catctaccta ttcatatacg ctagtgtcat agtcctgaaa atcatctgca
     3361 tcaagaacaa tttcacaact cttatacttt tctcttacaa gtcgttcggc ttcatctgga
     3421 ttttcagcct ctatacttac taaacgtgat aaagtttctg taatttctac tgtatcgacc
     3481 tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat
     3541 ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga
     3601 gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac
     3661 caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat
     3721 caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata
     3781 acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt
     3841 cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt
     3901 ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca
     3961 gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt
     4021 cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt atacatatca
     4081 gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggctttt
     4141 agtaagccgg atccacgcgg cgtttacgcc ccccctgcca ctcatcgcag tactgttgta
     4201 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     4261 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     4321 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     4381 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     4441 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     4501 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     4561 cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat
     4621 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     4681 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     4741 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     4801 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     4861 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     4921 gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac
     4981 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg
     5041 cgtcgggtga tgctgccaac ttagtcgact acaggtcact aataccatct aagtagttga
     5101 ttcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     5161 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
     5221 aagtggttga tctagagggc ccgcggttcg aacgtctctt gatcatatgg cgcgccctcg
     5281 aggtcgacgg tatcgataag ctcgcttcac gagattccag caggtcgagg gacctaataa
     5341 cttcgtatag catacattat acgaagttat attaagggtt ccaagcttaa gcggccgcgt
     5401 ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag
     5461 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     5521 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     5581 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     5641 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     5701 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     5761 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     5821 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     5881 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     5941 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
     6001 tgaaccgtca gatccgctag cgccaccatg agcgagctga ttaaggagaa catgcacatg
     6061 aagctgtaca tggagggcac cgtggacaac catcacttca agtgcacatc cgagggcgaa
     6121 ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc
     6181 cccttcgcct tcgacatcct ggctactagc ttcctctacg gcagcaagac cttcatcaac
     6241 cacacccagg gcatccccga cttcttcaag cagtccttcc ctgagggctt cacatgggag
     6301 agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag
     6361 gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcacatc caacggccct
     6421 gtgatgcaga agaaaacact cggctgggag gccttcaccg agactctgta ccccgctgac
     6481 ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag ccatctgatc
     6541 gcaaacatca agaccacata tagatccaag aaacccgcta agaacctcaa gatgcctggc
     6601 gtctactatg tggactacag actggaaaga atcaaggagg ccaacaacga gacctacgtc
     6661 gagcagcacg aggtggcagt ggccagatac tgcgacctcc ctagcaaact ggggcacaaa
     6721 ctcaattaaa catgtttaag ggttccggtt ccactaggta caattcgata tcaagcttat
     6781 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6841 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6901 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6961 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     7021 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     7081 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     7141 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     7201 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     7261 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     7321 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     7381 ccgtcgacct cgatcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     7441 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     7501 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7561 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     7621 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
     7681 ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     7741 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
     7801 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
     7861 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
     7921 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7981 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8041 gatccctcag acccttttag tcagtgtgga aaatctctag cagcatgtga gcaaaaggcc
     8101 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8161 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8221 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8281 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8341 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8401 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8461 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8521 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8581 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8641 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8701 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8761 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8821 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8881 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8941 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9001 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9061 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9121 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9181 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9241 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9301 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9361 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9421 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9481 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9541 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9601 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9661 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9721 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9781 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9841 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgac
//
