LOCUS       sequence-367342-       10832 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shApc-expressing lentiviruses with varius levels of expression of 
            SIINFEKL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10832)
  AUTHORS   Westcott PMK, Sacks NJ, Schenkel JM, Ely ZA, Smith O, Hauck H, 
            Jaeger AM, Zhang D, Backlund CM, Beytagh MC, Patten JJ, Elbashir R, 
            Eng G, Irvine DJ, Yilmaz OH, Jacks T
  TITLE     Low neoantigen expression and poor T-cell priming underlie early 
            immune escape in colorectal cancer.
  JOURNAL   Nat Cancer. 2021 Oct;2(10):1071-1085. doi: 
            10.1038/s43018-021-00247-z. Epub 2021 Sep 30.
  PUBMED    34738089
REFERENCE   2  (bases 1 to 10832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s43018-021-00247-z"; journalName: "Nat Cancer"; date: 
            "2021-10"; volume: "2"; issue: "10"; pages: "1071-1085"
COMMENT     Sequence Label: dimSIIN
FEATURES             Location/Qualifiers
     source          1..10832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     51..72
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(191..211)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        196..525
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      376..511
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     438..457
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        573..620
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             639..1013
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1143..1264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1313..1329)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1313..1329)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1326..1348)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1337..1353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1361..1367,1368..1385,1386..1391))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1406..1427
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1544..1561)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1715..2303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1795..1814)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2474..3265,3266..3334))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3097..3116
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3335..3439)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3514..3533)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3705..4084
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4086..4284
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4239..4259
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4302..4482
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4529..4654
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5147..5380
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5565..5609
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5907..6024
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    6074..6208
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6224..6247)
                     /codon_start=1
                     /product="antigenic CD8+ T cell epitope from chicken 
                     ovalbumin"
                     /label=OVA peptide
                     /translation="SIINFEKL"
     CDS             complement(6260..6955)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(6535..6554)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6804..6822
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        6993..8171
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7224..8162
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8119..8139
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(8211..8213,8214..8216,8217..8930)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8211..8213,8214..8216,8217..8927)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8256..8277)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8517..8536)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8864..8885
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     ncRNA           8974..9095
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           9123..9137
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           9165..9291
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     misc_feature    9391..9979
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9444..9464)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9862..9873)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10051..10284
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10310..10327)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10316..10540
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10586..10832,1..182)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10673..10692)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
       61 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
      121 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
      181 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
      241 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
      301 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
      361 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
      421 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
      481 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
      541 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
      601 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
      661 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
      721 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
      781 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
      841 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
      901 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
      961 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
     1021 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     1081 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     1141 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     1201 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     1261 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     1321 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     1381 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     1441 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     1501 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1561 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1621 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1681 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1741 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1801 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1861 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1921 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1981 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2041 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2101 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2161 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2221 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2281 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2341 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2401 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2461 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     2521 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     2581 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     2641 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     2701 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     2761 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     2821 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     2881 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     2941 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     3001 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     3061 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     3121 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     3181 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     3241 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     3301 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     3361 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     3421 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     3481 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     3541 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     3601 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     3661 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
     3721 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     3781 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     3841 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     3901 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     3961 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     4021 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     4081 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     4141 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     4201 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     4261 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc
     4321 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     4381 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     4441 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     4501 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     4561 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     4621 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     4681 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     4741 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     4801 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     4861 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     4921 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     4981 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     5041 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     5101 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     5161 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     5221 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     5281 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     5341 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     5401 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     5461 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     5521 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     5581 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     5641 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     5701 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     5761 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     5821 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     5881 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     5941 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     6001 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     6061 ttaattaagg tacgatccag acatgataag atacattgat gagtttggac aaaccacaac
     6121 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     6181 aaccattata agctgcaata aacaagttgg atccgtcgca ttacagtttt tcaaagttga
     6241 ttatactctc gagctgctcc ttgtacagct cgtccatgcc gccggtggag tggcggccct
     6301 cggagcgttc gtactgttcc accacggtgt agtcctcgtt gtgggaggtg atgtccaact
     6361 tgcggtcgac gttgtaggcg ccgggcatct gcacgggctt cttggccttg taggtggtct
     6421 tgaagtccgc caggtagcgg ccgccgtcct tcaggcgcag ggccatctta atgtcgccct
     6481 tcagcacgcc gtcctcgggg tacaaccgct cggtggacgc ttcccagccc attgtcttct
     6541 tctgcattac ggggccgtca ggagggaagt tggtgccgcg gagcttcacc ttgtagatca
     6601 gggtgccgtc ctccagggag gtgtcctggg tcacggtcac ggcgccgccg tcctcgaagt
     6661 tcatcacgcg ctcccacttg aagccctcgg ggaaggactg cttatagtag tcggggatgt
     6721 cggcggggtg cttggtgaag gccctggagc cgtacatgaa ctgaggggac aggatgtccc
     6781 aggagaaggg cagggggcca cccttggtca ccttcagctt ggcggtctgg gtgccctcgt
     6841 aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcatg gagccctcca
     6901 tgtgcacctt gaaccgcatg aactccttga tcactgcctc gcccttgctc accatggtgg
     6961 tcgatcagtg tgagggagtg taaagctggt ttggctccgg tgcccgtcag tgggcagagc
     7021 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
     7081 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     7141 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     7201 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
     7261 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc
     7321 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
     7381 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa
     7441 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     7501 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     7561 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     7621 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     7681 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     7741 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     7801 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     7861 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     7921 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     7981 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga
     8041 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     8101 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc
     8161 aggtgtcgtg actaactcga acgctagctg tgcgatcgtt ttcgaccacc atggtgagca
     8221 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     8281 acggccacaa gttcagcgtg tctggcgagg gcgagggcga tgccacctac ggcaagctga
     8341 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     8401 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     8461 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     8521 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     8581 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     8641 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     8701 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     8761 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     8821 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     8881 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa tgacgtacgg
     8941 ctaagcctcg actagggata acagggtaat tgtttgaatg aggcttcagt actttacaga
     9001 atcgttgcct gcacatcttg gaaacacttg ctgggattac ttcttcaggt taacccaaca
     9061 gaaggctcga gaaggtatat tgctgttgac agtgagcgcc accaataaat tacagtctta
     9121 tagtgaagcc acagatgtat aagactgtaa tttattggtg ttgcctactg cctcggaatt
     9181 caaggggcta ctttaggagc aattatcttg tttactaaaa ctgaatacct tgctatctct
     9241 ttgatacatt tttacaaagc tgaattaaaa tggtataaat taaatcactt ttttcaattc
     9301 taccaaacgt tgtttgtttg gggttgaatt actctacatg tttaagggtt ccggttccac
     9361 taggtacaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     9421 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     9481 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     9541 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     9601 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     9661 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     9721 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     9781 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     9841 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     9901 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     9961 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
    10021 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
    10081 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
    10141 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    10201 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    10261 agtcagtgtg gaaaatctct agcagggccc ggcgaaaccc gctgatcagc ctcgactgtg
    10321 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
    10381 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
    10441 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
    10501 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
    10561 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
    10621 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    10681 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    10741 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    10801 tagggtgatg gttcacgtag tgggccatcg cc
//
