LOCUS       sequence-367343-       18879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  B6J Rosa26-cre dre reporter 1.1 allele targeting vector, low copy 
            number.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18879)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     Miscellaneous gene targeting vector plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pB6-Rosa26-cre_dre_reporter_1_1
FEATURES             Location/Qualifiers
     source          1..18879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(132..149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(266..811)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(346..365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(976..1767,1768..1836))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1599..1618
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1837..1941)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2009..2027
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2065..2087)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2187..2206
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2400..2422
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2414..2431
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2415..2431
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2735..2783
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     2808..2827
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     primer_bind     2862..2882
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2864..2882
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    2944..3168
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     intron          3242..3307
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(3384..3956)
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     promoter        complement(3983..4482)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     misc_feature    4713..9053
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     protein_bind    9112..9145
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        9209..9708
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     promoter        9720..9767
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9786..10589
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9849..9868)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10459..10478
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10627..10851
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    10906..10939
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     enhancer        10975..11354
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11356..11633
                     /label=chicken beta-actin promoter
     intron          11634..12650
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     12574..12596
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     12658..12677
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(12705..12738)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(12802..12835)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(join(12845..13555,13556..13558,13559..13561))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(12845..13555,13556..13558,13559..13561))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(12887..12908)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     13236..13255
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     13495..13516
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    13565..13598
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    13664..13697
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    13698..13729
                     /label=rox
                     /bound_moiety="Dre recombinase"
     CDS             complement(14214..14225)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    14362..14586
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    14752..14873
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    14985..15106
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    15218..15339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    15351..15382
                     /label=rox
                     /bound_moiety="Dre recombinase"
     CDS             15383..15436
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             15437..16867
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             complement(17350..17361)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    17493..17717
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct
       61 tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata
      121 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa
      181 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa
      241 gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg
      301 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
      361 ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
      421 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca
      481 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta
      541 tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca
      601 gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac
      661 tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt
      721 ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca
      781 agagattacg cgcagaccaa aacgatctca agaagatcat cttattaagg ggtctgacgc
      841 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
      901 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
      961 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     1021 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     1081 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     1141 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     1201 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     1261 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
     1321 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     1381 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     1441 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     1501 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     1561 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
     1621 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     1681 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     1741 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     1801 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     1861 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     1921 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     1981 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     2041 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     2101 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     2161 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     2221 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     2281 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     2341 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     2401 ccagtcacga cgttgtaaaa cgacggccag tgccaagctt tcgcgagctc gacctgcagc
     2461 caagcttggg tcgtggacct gaccccacgc aacgcccaaa ataataaccc ccacgaacca
     2521 taaaccattc cccatggggg accccgtccc taacccacgg ggccagtggc tatggcaggg
     2581 cctgccgccc cgacgttggc tgcgagccct gggccttcac ccgaacttgg ggggtggggt
     2641 ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca
     2701 gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac acccgtgcgt
     2761 tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg tctccttccg
     2821 tgtttcagtt agcctccccc atctcccggg caaacgtgcg cgcaattaac cctcactaaa
     2881 gggaacaaaa gctgggtacc gggccccccc tcgagcccca gctggttctt tccgcctcag
     2941 aagccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa tcctccccct
     3001 tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga caatgcgatg
     3061 caatttcctc attttattag gaaaggacag tgggagtggc accttccagg gtcaaggaag
     3121 gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagtc gaggctgatc
     3181 agcgagctct aggatctgca ttccaccact gctcccattc atcagttcca taggttggaa
     3241 tctaaaatac acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaatttta
     3301 tatttacctt agagctttaa atctctgtag gtagtttgtc caattatgtc acaccacaga
     3361 agtaaggttc cttcacaaag agatcgcctg acacgatttc ctgcacaggc ttgagccata
     3421 tactcataca tcgcatcttg gccacgtttt ccacgggttt caaaattaat ctcaagttct
     3481 acgcttaacg ctttcgcctg ttcccagtta ttaatatatt caacgctaga actcccctca
     3541 gcgaagggaa ggctgagcac tacacgcgaa gcaccatcac cgaacctttt gataaactct
     3601 tccgttccga cttgctccat caacggttca gtgagactta aacctaactc tttcttaata
     3661 gtttcggcat tatccacttt tagtgcgaga accttcgtca gtcctggata cgtcactttg
     3721 accacgcctc cagcttttcc agagagcggg ttttcattat ctacagagta tcccgcagcg
     3781 tcgtatttat tgtcggtact ataaaaccct ttccaatcat cgtcataatt tccttgtgta
     3841 ccagattttg gcttttgtat acctttttga atggaatcta cataaccagg tttagtcccg
     3901 tggtacgaag aaaagttttc catcacaaaa gatttagaag aatcaacaac atcatcagga
     3961 tccatggcga ggacctgcag gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg
     4021 cagacgtgcg cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg
     4081 ccccgcccct gagcccgccc ctgagcccgc ccccggaccc accccttccc agcctctgag
     4141 cccagaaagc gaaggagcaa agctgctatt ggccgctgcc ccaaaggcct acccgcttcc
     4201 attgctcagc ggtgctgtcc atctgcacga gactagtgag acgtgctact tccatttgtc
     4261 acgtcctgca cgacgcgagc tgcggggcgg gggggaactt cctgactagg ggaggagtag
     4321 aaggtggcgc gaaggggcca ccaaagaacg gagccggttg gcgcctaccg gtggatgtgg
     4381 aatgtgtgcg aggccagagg ccacttgtgt agcgccaagt gcccagcggg gctgctaaag
     4441 cgcatgctcc agactgcctt gggaaaagcg cctcccctac ccggtagaat tcgccaatga
     4501 caagacggag ccatggcaat gttcaagcag gaccaaatgt ggtgcagtgt tgagggcaat
     4561 ctgggaaggt tccttaagaa gttatgttct gagaccattc tcagtggctc aacaacactt
     4621 ggtcaaaaat tttaattctc ccctcagaga aatggagtag ttactccact ttcaagttcc
     4681 ttataagctt accatcaacc ttatagtaca ctctagatgt cctgaaatat ttctatcaga
     4741 acaaggtagt ataaagctgg taggtataca aaacgctaga ctagtttcta tccctgaccc
     4801 ttaatctgct agtatatccg taggaagttg cttaagtgcc actagtacca acagcctctg
     4861 ttagagtgaa cagtagattt taatgaaggg ccaataaaga ccacttaaga ctactgactg
     4921 aattaaaaat ccacccagtg gagttagtat tggttatagt tatataacaa aatatccaac
     4981 ttagccaggc gtggtggtgc acgcctttaa tcccggcact caggaggcag aggcaggcgg
     5041 atttctgagt tcgaggccag cctggtctac aaagtgagtt ccaggacagc cagggctata
     5101 cagagaaacc ctgtctcgaa aaaccaaaaa aaaaaaaaaa aaggaaaaaa aatatggctt
     5161 gaaaacatgc tattgttgtc aaaaggagat ccaaacaagt atttctttga acaaaaatta
     5221 tgtagaaaac agggtgataa agaacaaggg ctttttaaaa aaggccagca ttatttgaga
     5281 tatacaagtg actccaattc aagcctaaag gccactcaat gctcactaac agtgctaatc
     5341 agaatcaatt ccttagccca gtcaaaaata gtcctgaata tcttctggat aaacatgtcc
     5401 tccaacatat aaactagtat acactaacaa aacgtctcaa cttcaaggtg aaatgcttga
     5461 ctcctagact tgtgacccag caaagtgctc tataggtagg gttactaggt cagagagtct
     5521 tgcctgcaaa ccacaattat acaattccac caaatgtaag acatgtaaaa atgacttatt
     5581 tagctcaggt tttgaaaatt aaaaatcaaa cccacaaaga cctaactttc acattaagta
     5641 caaatgttta acatatataa catgttttgc cttgatatat gaaatcatat gacatcacct
     5701 gacaggaaac ttaagtttat ttgaacaaca atcagcctaa ggtaggccta gcacatgatc
     5761 aggatagtgc agggaaaccc aaagaagtgc ttctgagtat aaaccaagag acctcgaaat
     5821 agcagctttg ttctgtatct catgggcttt aactatcact tgaaaacaat ttcacaaatc
     5881 acaaaaccaa catttgttta gtttccaact ctgcctattc accggagaaa tccatgtccc
     5941 aatttgatgg gggaaaactt gaatgaaata ttatttggaa tattttaata tccctgtgta
     6001 tatgcagatg gtttaaagac aggtaacctt aactgtagtt tcttggttgt ctgccttgtt
     6061 ttttgaaata tgctctcacc aggagcctgc caagtaacta ctcttgtgtg ctcagcaagt
     6121 cctagggatc ccttaagcat gctctaacag gcctggcttt tttttttaaa aagtaggttg
     6181 cggtgataat aattcaagtt ttcatcactt gaaacacatt ttaccaacta tcacccaagc
     6241 tcccttagtg tgtcccctat aaaagaatct gacctgcaag ttccaaaagt attactacat
     6301 tttaacctta gtattcagga gacaaaagca ggcagaactt tagtctacat atcttgttgc
     6361 agatcaggca gagatacata gtgagaccct gcctcaggaa aagctatcat ttttatattc
     6421 acagtaagca aattaacatt aaaagtcaga aattcttaat ttgactttgg ctgtgaagaa
     6481 tttggattct ctggatttga aactagaaaa tctgattaag cgatgcaaca gtttctgtat
     6541 actaaaactc taagaccctt ggttctaaag ataccacatt taaaaaaaaa gttaagtata
     6601 agacaaaaag ccttaatagc tcttttatag gggagggact catttaatat tagtccacct
     6661 cactcctcat aactatttta tgaggtttat tatataaccc attttttaaa aaaaaaatta
     6721 aaggacaatt ttagtgtttg aaagatttcc caaccccacc ctggaaatca ggctgcaaat
     6781 ctcagcagct gcccttagaa ggcaaaaagc catgccattt aagccatggg aagttagtag
     6841 caaacaagag accacatggc aggaaggagg ctttaaagaa agcccacagt gtcaaaagac
     6901 ccaaccaaca gcagagacag aaaaaataat acccaaatct catcagaaag gtagacggat
     6961 ttagccacat ccatagtggc tcattaggga atgcttcaat tataatttta tgaaatctca
     7021 gagcccccct ccccaacagg gtttctgtgt gtattcctgg ctatcctaga tagaacttga
     7081 tgtgtagacc aggctgggct caaactcaga gatctgcctg tctctgcctc cagagtgctg
     7141 ggattaaagg cataaaggca agcaccacca ctggctggct aaactctggc cctacatttt
     7201 tgaaatccaa aattatcatt acttgctttc atgtttagta atggctgcag acttagcttt
     7261 cagctttgta tagatgaagc acaaaagtta agtaaagcta gcagtttaaa caaataggat
     7321 actagaagtt caatttttat ttctttattg cttagtgtaa gagaaaagac attaaagtct
     7381 gcattggtct tctgtatccc acaagtctgc agttatggct cctctgtcca cagttacact
     7441 tcaaataaaa attagttcct tggtaacatt tccagtgctt aaagagtaat ttgattattg
     7501 gctaccatat tggaacaaac acaaagtatt tcattacttc agtaaattct actggagaca
     7561 gtattctctt taaaaaaaaa aaaaagaaga agaaggagga tttatttatt taatgtgaat
     7621 acacttgtgg tcttcagaca caccagaaga gggcatcaga tcccattaca gatggttgtg
     7681 agccaccatg tggttgctgg gaattgaact caggacttct ggaagagcag tctgtgctct
     7741 tgaactccga gccacctctc cagcccctgg atagtattat tatagagcac aagcacacac
     7801 aacacaacaa cctaaaaacc aagcttcaca aatacatttg ttgcttaatc attttacttg
     7861 tgtttggcta caagtcaagc aaaattatag gtcctgaaga agcttggcaa aatcacattt
     7921 agaccagcaa taacgtgtag aatgccatga gtcaagccag tccaagagaa agcactgctt
     7981 aaatagaaaa aatacaagct cacaagacct taggtcagga aagacaacaa acacctgaac
     8041 tttgcattcc aaaaggaacc accttttaca gatgtgtaca atgacaaaat tttagtaagc
     8101 agtaatcaat accatgtggc tcaataatga aatatacctt ttaatgtctt aaaatatcag
     8161 cctggcaata tgtaagatac atcaggtaat attgggggag gagacatcca cctggaaacc
     8221 attaatggtt aatagctcag tttataaatg gagaaaaagg agagaggcat tcatgggagt
     8281 ggaaagttaa gctttaggtt ggattctcaa tacatctatt gttgtaaaac tccctggact
     8341 gagaataggc ccaaatgtgg aacaccacct gacgggagag gtgatagaca ctgaaaatta
     8401 aggatcaagg caaaggatca acaaaaagtg tactaaggag ttataaaaga actgcagtgt
     8461 tgaggcccca gctacagcct cgatttgtgg tgtatgtaac taatctgtct ggtttcatga
     8521 gtcatcagac ttctaagatc aggaaaggga aaatgccaat gctctgtcta ggggttggat
     8581 aagccagtat aataaatgaa aatggggcta aaatgagtgt tctaaaatac cttttgataa
     8641 ggctgcagaa ggagcgggag aaatggatat gaagtactgg gctctttaaa aatgattaaa
     8701 attctgctta catagtctaa ctcgcgacac tgtaatttca tactgtagta aggatctcaa
     8761 gcaggagagt ataaaactcg ggtgagcatg tctttaatct acctcgatgg aaaatactcc
     8821 gaggcggatc acaagcaata ataacctgta gttttgctgc ataaaacccc agatgactac
     8881 ctatcctccc attttcctta tttgccccta ttaaaaaact tcccgacaaa accgaaaatc
     8941 tgtgggaagt cttgtccctc caattttaca cctgttcaat tcccctgcag gacaacgccc
     9001 acacaccagg ttagccttta agcctgccca gaagactccc gcccatcttc tagggcgcgc
     9061 cgatcttcga acatgttaat taaggtatcg ataagcttga tatcgaattc cgaagttcct
     9121 attctctaga aagtatagga acttcaggtc tgaagaggag tttacgtcca gccaagctag
     9181 cttggctgca ggtcgtcgaa attctaccgg gtaggggagg cgcttttccc aaggcagtct
     9241 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     9301 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
     9361 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     9421 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
     9481 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     9541 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
     9601 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
     9661 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagcctg
     9721 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
     9781 aaaccatggg atcggccatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     9841 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     9901 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     9961 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
    10021 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
    10081 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
    10141 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
    10201 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
    10261 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
    10321 cgcgcatgcc cgacggcgat gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
    10381 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
    10441 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
    10501 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
    10561 tctatcgcct tcttgacgag ttcttctgag gggatcaatt ctctagagct cgctgatcag
    10621 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
    10681 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
    10741 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
    10801 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
    10861 cggaaagaac cagctggggc tcgactagag cttgcggaac ccttcgaagt tcctattctc
    10921 tagaaagtat aggaacttca tcagtcaggt acataatgga tccggcgcgc cgtcgacatt
    10981 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
    11041 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
    11101 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
    11161 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
    11221 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
    11281 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
    11341 tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
    11401 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
    11461 cggggggggg gggggggcgc gcgccrgggg ggggggggsg gggsgrgggg sggggsgggg
    11521 sgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
    11581 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
    11641 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
    11701 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
    11761 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
    11821 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
    11881 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
    11941 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
    12001 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
    12061 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
    12121 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
    12181 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
    12241 ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc
    12301 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
    12361 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
    12421 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
    12481 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
    12541 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
    12601 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
    12661 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcataact tcgtatagca
    12721 tacattatac gaagttattg ggttgacggt gacgcttggc agttcggttc tatcgttaaa
    12781 tactggtctt cttacttcga cataacttcg tatagtacac attatacgaa gttataccat
    12841 ggctcttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
    12901 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt
    12961 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
    13021 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc
    13081 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct
    13141 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
    13201 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
    13261 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
    13321 gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga
    13381 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc
    13441 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc
    13501 cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca
    13561 tggtataact tcgtataatg tatgctatac gaagttatat ccaagtacga ggaccagtgc
    13621 ttcaccgata tcccccaact gccaggcctc cccctccacc catataactt cgtataatgt
    13681 gtactatacg aagttattaa ctttaaataa tgccaattat ttaaagttat aactagataa
    13741 ggatccaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
    13801 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
    13861 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
    13921 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
    13981 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
    14041 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
    14101 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
    14161 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
    14221 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
    14281 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catccggata
    14341 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
    14401 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
    14461 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
    14521 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
    14581 ctatggcttc tgaggcggaa agaaccagct ggggctcgac tagagcttgc ggaaccctta
    14641 gctagaatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
    14701 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
    14761 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    14821 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaactagaa
    14881 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
    14941 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
    15001 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    15061 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacta gaatcataat
    15121 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
    15181 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
    15241 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    15301 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ctagaccgga taactttaaa
    15361 taatgccaat tatttaaagt tagagggcag aggaagtctg ctaacatgcg gtgacgtcga
    15421 ggagaatcct ggcccaatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt
    15481 caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga
    15541 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct
    15601 gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa
    15661 gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga
    15721 gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca
    15781 ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc
    15841 cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga
    15901 cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct
    15961 ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta
    16021 cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta
    16081 cgagcgctcc gagggccgcc accacctgtt cctggggcat ggcaccggca gcaccggcag
    16141 cggcagctcc ggcaccgcct cctccgagga caacaacatg gccgtcatca aagagttcat
    16201 gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga
    16261 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
    16321 ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta
    16381 cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa
    16441 gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc
    16501 cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga
    16561 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc
    16621 ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca
    16681 ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta
    16741 ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
    16801 acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg acgagctgta
    16861 caagtaataa ctagataaaa gcttcctctg gattacaaaa tttgtgaaag attgactggt
    16921 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
    16981 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
    17041 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
    17101 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
    17161 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
    17221 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
    17281 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
    17341 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
    17401 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
    17461 tccccgcact agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
    17521 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
    17581 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
    17641 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
    17701 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga ctagagcttg
    17761 cggaaccctt aattaaaaag actggagttg cagatcacga gggaagaggg ggaagggatt
    17821 ctcccaggcc cagggcggtc ctcagaagcc aggaggcagc agagaactcc cagaaaggta
    17881 ttgcaacact cccctccccc ctccggagaa gggtgcggcc ttctccccgc ctactccact
    17941 gcagctccct tactgataac aactcagagc gactttggga gagcaagtgc ttcctgcctc
    18001 caaaacagcc caactgagcc ctcgctcctt ccctccactc cccggagtgc gcgatggagg
    18061 tctggctcag cacgcccctc ttgaggcaac tcaagtcgga aacgtgcttg cacccgcccc
    18121 gcagccgctc agccctactg cccgtccccg cccccagcgc gcgcttcctg ccacgttgcg
    18181 caggggcgcg cggccagact ctgcggcgcg gggccgaggg gagggccgga acctgggagc
    18241 gcctcctcgc cgcccccgct ggccggcgga tggactcaac ttgcacgaac acgagccaat
    18301 ggcaagggcc agttttctgg gccccgagag ccaatcagac gacgaggccc ggccggcggc
    18361 gggtaaaacg actcccccag aggaagggga gggtgggcgg ccgctcgcgc gcgagctact
    18421 ttcgctgacc cycccycccc ccccccgccc cgccagaggc cgaccgcgcc cgcacgtcca
    18481 gctcgcctca ccccacctac ctcccgcccc acccagtggg cagagcgagg ctgccggcgg
    18541 ctgcgcactc cggctgccgt taactgacag gcgccttacg ccaaccaaaa cacgccattt
    18601 gtgttttcac acacggcggg aggaaaagaa gccaatcagc gacgagacgt cggccggaag
    18661 cgctcctccg ctgccccccc ccccccgagc catggccgcg tccggtggag acttttccgc
    18721 tcccttctcc ctccccctcc ggttgctgca gggcggaccg cattcctgcc caccacccgc
    18781 ttgccccttc cagcgtcacg actcgtaccc ggctgtctca cagaacggct ccaccacgct
    18841 cggagggcct gccatttaaa tatttcgata agccaggtt
//
