LOCUS       sequence-367347-       14582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express VPS13C truncation mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14582)
  AUTHORS   Cai S, Wu Y, Guillen-Samander A, Hancock-Cerutti W, Liu J, De 
            Camilli P
  TITLE     In situ architecture of the lipid transport protein VPS13C at 
            ER-lysosome membrane contacts.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Jul 19;119(29):e2203769119. doi: 
            10.1073/pnas.2203769119. Epub 2022 Jul 13.
  PUBMED    35858323
REFERENCE   2  (bases 1 to 14582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-07-19"; volume: "119(29):e2203769119. 
            doi"; pages: " 10.1073/pnas.2203769119. Epub 2022 Jul 13"
COMMENT     Sequence Label: VPS13C^halo∆1235-1748
FEATURES             Location/Qualifiers
     source          1..14582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      597..606
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4809..5696
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     polyA_signal    11370..11491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11407..11426)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11461..11480
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(11498..11953)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11635..11656)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11847..11866
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        11980..12084
                     /gene="bla"
                     /label=AmpR promoter
     promoter        12086..12443
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        12086..12277
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(12109..12129)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      12294..12429
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             12478..13272
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             12478..13272
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12532..12551)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     13142..13161
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(13460..13479)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    13504..13551
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      13880..14468
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14369..14388
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      601 ccatggtcct ggagagcgtg gtcgccgatc tgctgaaccg attcctgggg gattacgtgg
      661 agaacctgaa caagtcacag ctgaaactgg gaatctgggg aggaaacgtg gccctggaca
      721 atctgcagat caaggagaac gccctgagcg agctggacgt gcccttcaag gtgaaggccg
      781 gccagatcga taagctgacc ctgaagatcc cttggaagaa tctgtacgga gaggcagtgg
      841 tggccacact ggagggcctg tatctgctgg tggtgccagg agccagcatc aagtacgacg
      901 ccgtgaagga ggagaagtct ctgcaggatg tgaagcagaa ggagctgagc cggatcgagg
      961 aggccctgca gaaggcagca gagaagggaa cccactccgg agagtttatc tatggcctgg
     1021 agaacttcgt gtacaaggac atcaagcctg gccgcaagcg gaagaagcac aagaagcact
     1081 ttaagaagcc tttcaagggc ctggacagat ccaaggataa gccaaaggag gccaagaagg
     1141 ataccttcgt ggagaagctg gccacacagg tcatcaagaa tgtgcaggtg aagatcacag
     1201 acatccacat caagtatgag gacgatgtga ccgatccaaa gaggcccctg agctttggcg
     1261 tgacactggg cgagctgtcc ctgctgacag ccaacgagca ctggacccca tgcatcctga
     1321 atgaggccga caagatcatc tacaagctga tccgcctgga tagcctgtcc gcctattgga
     1381 acgtgaattg ttctatgagc taccagagat ccagggagca gatcctggac cagctgaaga
     1441 acgagatcct gacctctggc aacatccccc ctaattacca gtatatcttc cagcctatct
     1501 ccgcctctgc caagctgtat atgaatccat acgccgagag cgagctgaag acccccaagc
     1561 tggattgcaa catcgagatc cagaatatcg ccatcgagct gacaaagcca cagtatctgt
     1621 ctatgatcga cctgctggag agcgtggatt acatggtgag gaacgccccc taccgcaagt
     1681 ataagcctta cctgccactg cacaccaatg gccggagatg gtggaagtat gccatcgact
     1741 ctgtgctgga ggtgcacatc aggcgctaca cacagatgtg gagctggtcc aacatcaaga
     1801 agcaccggca gctgctgaag tcctataaga tcgcctacaa gaataagctg acccagtcca
     1861 aggtgtctga ggagatccag aaggagatcc aggacctgga gaagacactg gacgtgttca
     1921 acatcatcct ggcaaggcag caggcacagg tggaagtgat cagatccggc cagaagctga
     1981 ggaagaagtc tgccgacacc ggagagaaga gaggaggatg gttcagcggc ctgtggggca
     2041 agaaggagag caagaagaag gacgaggagt ctctgatccc tgagacaatc gacgatctga
     2101 tgaccccaga ggagaaggat aagctgttta ccgccatcgg ctattctgag agcacacaca
     2161 atctgaccct gcccaagcag tacgtggccc acatcatgac actgaagctg gtgtccacct
     2221 ctgtgacaat cagggagaac aagaatatcc ctgagatcct gaagatccag atcatcggcc
     2281 tgggaaccca ggtgtcccag cggccaggcg cccaggccct gaaggtggag gcaaagctgg
     2341 agcactggta tatcacaggc ctgagacagc aggacatcgt gcctagcctg gtggcctcca
     2401 tcggcgatac cacaagctcc ctgctgaaga tcaagttcga gacaaacccc gaggactccc
     2461 ctgccgatca gaccctgatc gtgcagtctc agccagtgga agtgatctac gacgccaaga
     2521 ccgtgaacgc cgtggtggag ttctttcagt ctaataaggg cctggatctg gagcagatca
     2581 ccagcgccac actgatgaag ctggaggaga tcaaggagcg caccgccaca ggcctgacac
     2641 acatcatcga gacacgcaag gtgctggacc tgcggatcaa cctgaagcct tcctatctgg
     2701 tggtgccaca gaccggcttc caccacgaga agtctgacct gctgatcctg gattttggca
     2761 cattccagct gaacagcaag gaccagggcc tgcagaagac cacaaattct agcctggagg
     2821 agatcatgga taaggcctac gacaagtttg atgtggagat caagaatgtg cagctgctgt
     2881 tcgccagagc cgaggagaca tggaagaagt gcaggtttca gcacccaagc acaatgcaca
     2941 tcctgcagcc catggacatc cacgtggagc tggccaaggc catggtggag aaggatatca
     3001 gaatggcaag gttcaaggtg tccggaggcc tgccactgat gcacgtgagg atctctgacc
     3061 agaagatgaa ggatgtgctg tatctgatga atagcatccc actgccccag aagtcctctg
     3121 cccagtcccc tgagaggcag gtgagctcca tcccaatcat cagcggagga acaaagggcc
     3181 tgctgggcac ctccctgctg ctggacacag tggagagcga gtccgacgat gagtacttcg
     3241 acgcagagga tggagagcca cagacctgta agtctatgaa gggcagcgag ctgaagaagg
     3301 ccgccgaggt gcctaacgag gagctgatca atctgctgct gaagtttgag atcaaggaag
     3361 tgatcctgga gttcaccaag cagcagaagg aggaggatac catcctggtg tttaacgtga
     3421 cacagctggg caccgaggcc acaatgagga ccttcgacct gacagtggtg tcttatctga
     3481 agaagatcag cctggattac cacgagatcg agggcagcaa gcgcaagccc ctgcacctga
     3541 tctctagctc cgacaagcct ggcctggatc tgctgaaggt ggagtatatc aaggccgaca
     3601 agaatggccc ttcctttcag accgccttcg gcaagacaga gcagaccgtg aaggtggcct
     3661 tctctagcct gaacctgctg ctgcagacac aggccctggt ggcctctatc aattacctga
     3721 ccacaatcat ccctagcgac gatcagtcta tcagcgtggc caaggaggtg cagatcagca
     3781 ccgagaagca gcagaagaac tccacactgc caaaggccat cgtgtcctct agagacagcg
     3841 atatcatcga ctttaggctg ttcgccaagc tgaacgcctt ttgcgtgatc gtgtgcaatg
     3901 agaagaacaa tatcgccgag atcaagatcc agggcctgga tagctccctg agcctgcagt
     3961 ccagaaagca gtccctgttc gccaggctgg agaatatcat cgtgaccgac gtggatccca
     4021 agacagtgca caagaaggcc gtgtctatca tgggcaacga ggtgttcagg tttaatctgg
     4081 acctgtatcc tgatgccacc gagggcgacc tgtacacaga tatgagcaag gtggacggcg
     4141 tgctgtccct gaacgtgggc tgcatccaga tcgtgtacct gcacaagttc ctgatgagcc
     4201 tgctgaattt tctgaacaat ttccagaccg ccaaggagtc cctgtctgcc gcaacagcac
     4261 aggcagcaga gagggcagcc acctccgtga aggacctggc acagaagtct ttccgcctgc
     4321 tgatggatat caatctgaag gcccctgtga tcatcatccc acagagctcc gtgagcccaa
     4381 acgccgtgat cgcagacctg ggcctgatta gggtggagaa taagttttct ctggtgccta
     4441 tggagcacta tagcctgcct ccagtgatcg acaagatgaa catcgagctg acccagctga
     4501 agctgagccg gacaatcctg caggcatccc tgccacagaa tgatatcgag atcctgaagc
     4561 ctgtgaacat gctgctgagc atccagagaa atctggcagc agcatggtac gtgcagatcc
     4621 caggcatgga gatcaagggc aagctgaagc ccatgcaggt ggccctgtcc gaggacgatc
     4681 tgaccgtgct gatgaagatc ctgctggaga acctgggcga ggcctctagc cagccttctc
     4741 caacccagag cgtgcaggag acagtgcgcg tgcggaaggt ggacgtgtcc tctgtgccat
     4801 ccgcgggagc agagatcgga accggcttcc cctttgatcc tcactatgtg gaggtgctgg
     4861 gcgagcgcat gcactacgtg gacgtgggac cccgggacgg aacacctgtg ctgttcctgc
     4921 acggcaaccc taccagctcc tacgtgtggc gcaatatcat ccctcacgtg gcaccaaccc
     4981 acaggtgcat cgcaccagac ctgatcggca tgggcaagag cgacaagccc gatctgggct
     5041 acttctttga cgatcacgtg aggtttatgg acgccttcat cgaggccctg ggcctggagg
     5101 aggtggtgct ggtcatccac gattggggat ccgccctggg attccactgg gcaaagagaa
     5161 acccagagag ggtgaagggc atcgccttta tggagttcat caggccaatc cctacctggg
     5221 acgagtggcc cgagtttgcc agagagacat ttcaggcctt cagaaccaca gacgtgggca
     5281 ggaagctgat catcgatcag aacgtgttca tcgagggcac actgccaatg ggagtggtgc
     5341 ggcccctgac cgaggtggag atggaccact atagggagcc ctttctgaat cctgtggatc
     5401 gggagccact gtggagattc ccaaacgagc tgccaatcgc aggagagcca gcaaatatcg
     5461 tggccctggt ggaggagtac atggactggc tgcaccagag cccagtgccc aagctgctgt
     5521 tctggggcac cccaggcgtg ctgatcccac ctgcagaggc agcaaggctg gccaagtccc
     5581 tgccaaactg taaggccgtg gatatcggcc ctggcctgaa cctgctgcag gaggacaatc
     5641 ccgatctgat cggctctgag atcgccagat ggctgagcac cctggagatc agcggatccg
     5701 cgggagacca cctgaaggag caggaggact ggacagatag caagctgtcc atgaatcaga
     5761 tcgtgagcct gcagttcgat tttcactttg agtctctgag catcatcctg tacaacaatg
     5821 acatcaacca ggagtccggc gtggcctttc acaatgattc tttccagctg ggcgagctga
     5881 ggctgcacct gatggcctct agcggcaaga tgttcaagga cggctccatg aacgtgtctg
     5941 tgaagctgaa gacctgcaca ctggacgatc tgagggaggg aatcgagagg gccacctccc
     6001 ggatgatcga tagaaagaac gaccaggata acaattcctc tatgatcgac atcagctata
     6061 agcaggataa gaatggctcc cagatcgacg ccgtgctgga taagctgtac gtgtgcgcct
     6121 ctgtggagtt tctgatgacc gtggccgact tctttatcaa ggcagtgcca cagagcccag
     6181 agaacgtggc caaggagaca cagatcctgc ctaggcagac cgccacaggc aaggtgaaga
     6241 tcgagaagga cgatagcgtg cgccctaata tgaccctgaa ggccatgatc acagacccag
     6301 aggtggtgtt tgtggcatcc ctgaccaagg cagatgcacc agccctgaca gcctctttcc
     6361 agtgcaacct gtccctgtct accagcaagc tggagcagat gatggaggca tccgtgcggg
     6421 acctgaaggt gctggcctgt ccatttctga gagagaagag gggcaagaat atcaccacag
     6481 tgctgcagcc ctgctccctg ttcatggaga agtgtacctg ggcctctggc aagcagaaca
     6541 tcaatatcat ggtgaaggag ttcatcatca agatcagccc tatcatcctg aacacagtgc
     6601 tgaccatcat ggccgccctg tccccaaaga ccaaggagga cggcagcaag gatacatcca
     6661 aggagatgga gaatctgtgg ggcatcaaga gcatcaacga ctacaatacc tggtttctgg
     6721 gcgtggatac cgccacagag atcacagagt ccttcaaggg catcgagcac tctctgatcg
     6781 aggagaactg cggcgtcgtg gtggagagca tccaggtgac actggagtgt ggcctgggac
     6841 acagaaccgt gccactgctg ctggccgagt ctaagttcag cggcaacatc aagaattgga
     6901 catccctgat ggcagcagtg gcagacgtga ccctgcaggt gcactactat aatgagatcc
     6961 acgccgtgtg ggagcctctg atcgagaggg tggagggcaa gcgccagtgg aacctgcggt
     7021 tggacgtgaa gaagaatccc gtgcaggata agagcctgct gcctggcgac gacttcatcc
     7081 ctgagccaca gatggccatc cacatcagct ccggcaacac aatgaatatc accatctcca
     7141 agtcttgcct gaacgtgttt aacaatctgg ccaagggctt ctccgagggc acagcctcta
     7201 cctttgacta tagcctgaag gatagagccc ccttcaccgt gaagaatgcc gtgggcgtgc
     7261 caatcaaggt gaagcccaac tgtaatctgc gcgtgatggg ctttcctgag aagagcgaca
     7321 tcttcgacgt ggatgccggc cagaacctgg agctggagta cgcctccatg gtgccatcta
     7381 gccagggcaa tctgtccatc ctgtctaggc aggagtcctc tttctttacc ctgacaatcg
     7441 tgccccacgg ctataccgag gtggccaaca tccctgtggc cagaccaggc cggagactgt
     7501 acaatgtgag gaaccccaat gccagccact ccgactctgt gctggtgcag atcgatgcca
     7561 ccgagggcaa caaagtgatc accctgcgca gccctctgca gatcaagaat cacttttcca
     7621 tcgccttcat catctataag ttcgtgaaga acgtgaagct gctggagaga atcggcatcg
     7681 ccaggcctga ggaggagttt cacgtgccac tggacagcta caggtgccag ctgttcatcc
     7741 agccagccgg catcctggag caccagtata aggagtccac cacatacatc tcttggaagg
     7801 aggagctgca cagaagcagg gaggtgcgct gcatgctgca gtgtccatct gtggaggtga
     7861 gctttctgcc cctgatcgtg aacaccgtgg ccctgcccga tgagctgtcc tacatctgta
     7921 cacacggcga ggactgggat gtggcctata tcatccacct gtaccctagc ctgaccctgc
     7981 gcaatctgct gccatattcc ctgaggtacc tgctggaggg aacagcagag acacacgagc
     8041 tggcagaggg aagcacagca gacgtgctgc acagccggat ctccggagag atcatggagc
     8101 tggtgctggt gaagtaccag ggcaagaact ggaatggcca ctttcgcatc cgggataccc
     8161 tgcccgagtt ctttcccgtg tgcttcagct ccgactccac agaggtgacc acagtggatc
     8221 tgtctgtgca cgtgaggagg atcggcagcc ggatggtgct gagcgtgttc agcccttatt
     8281 ggctgatcaa caagaccaca cgcgtgctgc agtaccggtc cgaggacatc cacgtgaagc
     8341 acccagccga ctttagagat atcatcctgt tcagcttcaa gaagaagaac atcttcacaa
     8401 agaataaggt gcagctgaag atcagcacct ccgcctggtc tagctccttt tccctggata
     8461 ccgtgggctc ttatggctgc gtgaagtgtc ccgccaacaa tatggagtac ctggtgggcg
     8521 tgtctatcaa gatgtctagc tttaacctga gcaggatcgt gaccctgaca ccattctgca
     8581 ccatcgccaa taagtcctct ctggagctgg aggtcggaga gatcgcatcc gacggatcta
     8641 tgcccacaaa caagtggaat tatatcgcca gctccgagtg tctgcctttc tggccagagt
     8701 ctctgagcgg caagctgtgc gtgcgggtgg tgggatgtga gggctctagc aagcccttct
     8761 tttacaacag acaggataat ggcaccctgc tgagcctgga ggacctgaac ggcggcatcc
     8821 tggtggatgt gaatacagcc gagcacagca cagtgatcac cttttccgac tatcacgagg
     8881 gctctgcccc tgccctgatc atgaaccaca ccccatggga tatcctgaca tacaagcagt
     8941 ccggctctcc cgaggagatg gtgctgctgc ctaggcaggc aaggctgttt gcatgggcag
     9001 accctaccgg aacaaggaag ctgacctgga catatgcagc aaacgtggga gagcacgacc
     9061 tgctgaagga tggctgtggc cagttcccat acgacgccaa tatccagatc cactgggtga
     9121 gctttctgga tggcagacag agggtgctgc tgttcaccga cgatgtggcc ctggtgtcca
     9181 aggccctgca ggcagaggag atggagcagg ccgattatga gatcacactg agcctgcact
     9241 ccctgggcct gagcctggtg aacaatgaga gcaagcagga ggtgtcctac atcggcatca
     9301 cctcctctgg cgtggtgtgg gaggtgaagc caaagcagaa gtggaagccc ttctcccaga
     9361 agcagatcat cctgctggag cagagctacc agaagcacca gatctcccgg gaccacggct
     9421 ggatcaagct ggataacaat tttgaggtga acttcgacaa ggatcccatg gagatgaggc
     9481 tgccaatccg ctcccccatc aagagggact ttctgtctgg catccagatc gagttcaagc
     9541 agagctccca ccagcgcagc ctgcgggcca gactgtactg gctgcaggtg gataaccagc
     9601 tgcctggcgc catgtttcca gtggtgttcc acccagtggc accaccaaag tctatcgccc
     9661 tggacagcga gcccaagcct tttatcgacg tgagcgtgat caccagattc aatgagtatt
     9721 ctaaggtgct gcagtttaag tacttcatgg tgctgatcca ggagatggcc ctgaagatcg
     9781 accagggctt tctgggcgcc atcatcgccc tgttcacccc taccacagat ccagaggccg
     9841 agcggagaag gacaaagctg atccagcagg acatcgatgc cctgaacgcc gagctgatgg
     9901 agacatccat gaccgacatg tctatcctga gcttctttga gcactttcac atctctcccg
     9961 tgaagctgca cctgagcctg tccctgggat ccggaggcga ggagtctgat aaggagaagc
    10021 aggagatgtt cgccgtgcac tctgtgaatc tgctgctgaa gagcatcggc gccaccctga
    10081 cagacgtgga cgatctgatc tttaagctgg cctactatga gatccgctat cagttctaca
    10141 agcgggatca gctgatctgg agcgtggtgc ggcactattc cgagcagttt ctgaagcaga
    10201 tgtacgtgct ggtgctgggc ctggacgtgc tgggaaaccc cttcggcctg atcagaggcc
    10261 tgagcgaggg agtggaggcc ctgttttatg agccattcca gggagcagtg cagggacccg
    10321 aggagtttgc agagggcctg gtcatcggcg tgcggtccct gttcggacac accgtgggag
    10381 gagcagcagg agtggtgagc agaatcaccg gatccgtggg caagggcctg gcagccatca
    10441 caatggacaa ggagtaccag cagaagcgcc gggaggagct gagcagacag cctagggact
    10501 ttggcgattc cctggccaga ggcggcaagg gcttcctgag gggagtggtg ggaggagtga
    10561 caggcatcat caccaagcca gtggagggag ccaagaagga gggagcagca ggcttcttta
    10621 agggcatcgg caagggcctg gtgggagcag tggccagacc cacaggcggc atcgtggaca
    10681 tggcctctag caccttccag ggcatccaga gagccgccga gagcaccgag gaggtgtcct
    10741 ctctgcgccc tccaaggctg atccacgagg acggcatcat ccggccctat gatagacagg
    10801 agtccgaggg ctctgatctg ctggagaacc acatcaagaa gctggagggc gagacataca
    10861 gatatcactg cgccatccct ggctccaaga agacaatcct gatggtgacc aatagacggg
    10921 tgctgtgcat caaggaggtg gagatcctgg gcctgatgtg cgtggactgg cagtgtccct
    10981 ttgaggattt cgtgttcccc ccttctgtga gcgagaacgt gctgaagatc agcgtgaagg
    11041 agcagggcct gttccacaag aaggactccg ccaatcaggg ctgcgtgaga aaggtgtacc
    11101 tgaaagacac cgccacagcc gagagggcct gtaacgccat cgaggatgcc cagagcaccc
    11161 gccagcagca gaaactgatg aaacagagct ccgtgagact gctgagacca cagctgcctt
    11221 cctgaggtac cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata
    11281 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
    11341 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
    11401 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    11461 gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg
    11521 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
    11581 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
    11641 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
    11701 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
    11761 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
    11821 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
    11881 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
    11941 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
    12001 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
    12061 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
    12121 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
    12181 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
    12241 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
    12301 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
    12361 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
    12421 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
    12481 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
    12541 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
    12601 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
    12661 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
    12721 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
    12781 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
    12841 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
    12901 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
    12961 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
    13021 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
    13081 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
    13141 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
    13201 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
    13261 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
    13321 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
    13381 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
    13441 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
    13501 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
    13561 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
    13621 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
    13681 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
    13741 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    13801 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    13861 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    13921 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    13981 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    14041 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    14101 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    14161 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    14221 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    14281 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    14341 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    14401 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    14461 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    14521 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc
    14581 at
//
