LOCUS       sequence-367358-        9752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Disruption of LINC Complex.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Lombardi ML, Jaalouk DE, Shanahan CM, Burke B, Roux KJ, Lammerding J
  TITLE     The interaction between nesprins and sun proteins at the nuclear 
            envelope is critical for force transmission between the nucleus and 
            cytoskeleton.
  JOURNAL   J Biol Chem. 2011 Jul 29;286(30):26743-53. doi: 
            10.1074/jbc.M111.233700. Epub 2011 Jun 7.
  PUBMED    21652697
REFERENCE   2  (bases 1 to 9752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.233700"; journalName: "J Biol Chem"; date: 
            "2011-07-29- 29"; volume: "286"; issue: "30"; pages: "26743-53"
COMMENT     Sequence Label: SS-HA-Sun1L-KDEL
FEATURES             Location/Qualifiers
     source          1..9752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2167..2187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2213..2237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2380..2406
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        3894..4105
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4118..4386
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      4415..4424
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4445..5107
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             5177..5230
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5231..5830
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5231..5250)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5727..5747
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5831..6419
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5884..5904)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6302..6313)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6493..6726
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6798..6919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6835..6854)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6889..6908
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6959..7094
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7127..7143)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7127..7143)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7140..7162)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7151..7167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7175..7181,7182..7199,7200..7205))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7220..7241
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7358..7375)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7529..8117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8288..9079,9080..9148))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8911..8930
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9149..9253)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9321..9339
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9377..9399)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9499..9518
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9712..9734
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9726..9743
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9727..9743
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     2221 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     2281 tctagagcta gcacaatgaa gtgggtaacc tttatttccc ttctttttct ctttagctcg
     2341 gcctattcca ggggtgtgtt tcgtcgagat gggcccgctt acccttacga tgtaccggat
     2401 tacgcactcg acgattccaa gggcatgcat agacctggcc ctcttccccc gagcccacct
     2461 ccaaaggttg atcacaaggc ttcccagtgg cctcaggaga gtgacatggg gcagaaggta
     2521 gcttctttga gtgcgcagtg ccacaaccat gatgagagac ttgcagagct gacagtcctg
     2581 cttcagaaac tacagatacg ggtagaccaa gtggatgacg gcagggaagg gctgtcactg
     2641 tgggtcaaga atgtggttgg acagcacctg caggagatgg gcaccataga accacctgat
     2701 gctaagactg acttcatgac tttccaccgt gaccatgaag tgcgtctctc caacttggaa
     2761 gatgttctta gaaaactgac agaaaaatct gaggctatcc agaaggagct ggaagaaacc
     2821 aagctgaaag caggcagcag ggatgaagag cagcccctcc ttgaccgtgt gcagcaccta
     2881 gaactggaac tgaacctgtt gaagtcacag ctgtcagact ggcagcatct gaagaccagc
     2941 tgtgagcagg ctggggcccg catccaggag actgtgcagc tcatgttctc tgaggatcag
     3001 cagggcggtt ccctcgagtg gctattagag aagctttctt ctcggttcgt gagcaaggat
     3061 gagctgcagg tgctcttaca tgaccttgag ctgaaactgc tgcagaatat cacacaccac
     3121 atcaccgtga caggacaggc cccgacatcc gaggctattg tgtctgccgt gaatcaggca
     3181 gggatttcag gaatcacaga agcgcaagca catatcattg tgaacaatgc tctgaagctg
     3241 tactcccaag acaagacggg gatggtggac tttgctctgg agtctggagg tggcagcatc
     3301 ctaagcactc ggtgctctga gacctatgag accaagacgg cactgctgag cctgtttggg
     3361 gtcccactgt ggtacttctc acagtcacct cgagtggtga tccagcccga catctaccca
     3421 gggaattgct gggcgttcaa aggttcccag gggtacctgg tggtgcggtt gtccatgaag
     3481 atctacccaa ccacactcac catggaacac attccaaaga cactatcacc cactggtaac
     3541 atctccagtg cccccaaaga ctttgcagtc tatggactgg aaacggagta tcaagaagag
     3601 gggcagcctc tgggacggtt cacctatgac caggaaggag actcactcca gatgttccac
     3661 acactggaaa gacctgacca agccttccag atagtagagc tccgggtcct gtccaactgg
     3721 ggccaccctg agtacacttg cctctaccgg ttccgagtcc acggagagcc catccagaaa
     3781 gatgagttgt agcttaagtt taaaccgctg atcagcctcg actatcgatg aattcgaatt
     3841 taaatcggat ccgcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga
     3901 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc
     3961 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     4021 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     4081 caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg
     4141 cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc
     4201 cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag
     4261 accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct
     4321 ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta
     4381 cagatccaag ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg
     4441 cctgcccgcc atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga
     4501 gctggtgggc ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag
     4561 caccaaaggc gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt
     4621 ctaccacttc ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa
     4681 cggcggctac accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag
     4741 cttcagctac cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg
     4801 cttccccgag gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga
     4861 gcacctgcac cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct
     4921 gcgcgacggc ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat
     4981 ccaccccagc atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct
     5041 gcacagcaac accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc
     5101 cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg
     5161 ctccaccgga tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     5221 tcccggccct atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     5281 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     5341 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     5401 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     5461 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     5521 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     5581 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     5641 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     5701 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     5761 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     5821 cggtgcctga aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5881 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5941 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     6001 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     6061 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     6121 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6181 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     6241 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6301 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6361 tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     6421 tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6481 aaaagggggg actggaaggg ctaattcact cccaacgaag ataagatctg ctttttgctt
     6541 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6601 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6661 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6721 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     6781 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6841 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6901 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     6961 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7021 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     7081 cttttttgga ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc
     7141 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7201 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7261 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7321 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7381 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7441 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7501 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7561 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7621 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7681 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7741 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7801 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7861 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7921 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7981 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8041 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8101 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8161 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8221 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8281 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8341 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8401 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8461 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8521 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8581 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8641 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8701 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8761 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8821 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8881 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8941 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9001 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9061 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9121 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9181 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9241 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     9301 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     9361 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     9421 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     9481 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     9541 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     9601 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     9661 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     9721 gacgttgtaa aacgacggcc agtgccaagc tg
//
