LOCUS       sequence-367371-        9905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector - ACE2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Kastenhuber ER, Mercadante M, Nilsson-Payant B, Johnson JL, Jaimes 
            JA, Muecksch F, Weisblum Y, Bram Y, Chandar V, Whittaker GR, 
            tenOever BR, Schwartz RE, Cantley L
  TITLE     Coagulation factors directly cleave SARS-CoV-2 spike and enhance 
            viral entry.
  JOURNAL   Elife. 2022 Mar 23;11. pii: 77444. doi: 10.7554/eLife.77444.
  PUBMED    35294338
REFERENCE   2  (bases 1 to 9905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-03-23"; volume: "11. pii: 77444. doi"; pages: " 
            10.7554/eLife.77444"
COMMENT     Sequence Label: pEGPN-ACE2
FEATURES             Location/Qualifiers
     source          1..9905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1683..1916
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2101..2145
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2443..2560
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2656..2867
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     promoter        5400..5899
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5435..5458)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5832..5851
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5936..6730
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5936..6730
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5990..6009)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6600..6619
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6793..7381
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6846..6866)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7264..7275)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7384..7400)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7385..7401)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7910..8090
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8152..8740)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8232..8251)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8911..9702,9703..9771))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9534..9553
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9772..9876)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
     1561 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1621 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1681 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1741 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1801 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1861 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1921 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1981 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2041 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2101 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2161 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2221 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2281 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2341 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2401 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2461 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2521 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2581 cagagatcca gtttggttag taccgggccc ggggatcagt gtgagggagt gtaaagctgg
     2641 tttaaaccct gcagggggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2701 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2761 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2821 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagcgc cggtgctaac
     2881 tcgaacgcta gctgtgcgat cgtttgccac catgtcaagc tcttcctggc tccttctcag
     2941 ccttgttgct gtaactgctg ctcagtccac cattgaggaa caggccaaga catttttgga
     3001 caagtttaac cacgaagccg aagacctgtt ctatcaaagt tcacttgctt cttggaatta
     3061 taacaccaat attactgaag agaatgtcca aaacatgaat aatgctgggg acaaatggtc
     3121 tgccttttta aaggaacagt ccacacttgc ccaaatgtat ccactacaag aaattcagaa
     3181 tctcacagtc aagcttcagc tgcaggctct tcagcaaaat gggtcttcag tgctctcaga
     3241 agacaagagc aaacggttga acacaattct aaatacaatg agcaccatct acagtactgg
     3301 aaaagtttgt aacccagata atccacaaga atgcttatta cttgaaccag gtttgaatga
     3361 aataatggca aacagtttag actacaatga gaggctctgg gcttgggaaa gctggagatc
     3421 tgaggtcggc aagcagctga ggccattata tgaagagtat gtggtcttga aaaatgagat
     3481 ggcaagagca aatcattatg aggactatgg ggattattgg agaggagact atgaagtaaa
     3541 tggggtagat ggctatgact acagccgcgg ccagttgatt gaagatgtgg aacatacctt
     3601 tgaagagatt aaaccattat atgaacatct tcatgcctat gtgagggcaa agttgatgaa
     3661 tgcctatcct tcctatatca gtccaattgg atgcctccct gctcatttgc ttggtgatat
     3721 gtggggtaga ttttggacaa atctgtactc tttgacagtt ccctttggac agaaaccaaa
     3781 catagatgtt actgatgcaa tggtggacca ggcctgggat gcacagagaa tattcaagga
     3841 ggccgagaag ttctttgtat ctgttggtct tcctaatatg actcaaggat tctgggaaaa
     3901 ttccatgcta acggacccag gaaatgttca gaaagcagtc tgccatccca cagcttggga
     3961 cctggggaag ggcgacttca ggatccttat gtgcacaaag gtgacaatgg acgacttcct
     4021 gacagctcat catgagatgg ggcatatcca gtatgatatg gcatatgctg cacaaccttt
     4081 tctgctaaga aatggagcta atgaaggatt ccatgaagct gttggggaaa tcatgtcact
     4141 ttctgcagcc acacctaagc atttaaaatc cattggtctt ctgtcacccg attttcaaga
     4201 agacaatgaa acagaaataa acttcctgct caaacaagca ctcacgattg ttgggactct
     4261 gccatttact tacatgttag agaagtggag gtggatggtc tttaaagggg aaattcccaa
     4321 agaccagtgg atgaaaaagt ggtgggagat gaagcgagag atagttgggg tggtggaacc
     4381 tgtgccccat gatgaaacat actgtgaccc cgcatctctg ttccatgttt ctaatgatta
     4441 ctcattcatt cgatattaca caaggaccct ttaccaattc cagtttcaag aagcactttg
     4501 tcaagcagct aaacatgaag gccctctgca caaatgtgac atctcaaact ctacagaagc
     4561 tggacagaaa ctgttcaata tgctgaggct tggaaaatca gaaccctgga ccctagcatt
     4621 ggaaaatgtt gtaggagcaa agaacatgaa tgtaaggcca ctgctcaact actttgagcc
     4681 cttatttacc tggctgaaag accagaacaa gaattctttt gtgggatgga gtaccgactg
     4741 gagtccatat gcagaccaaa gcatcaaagt gaggataagc ctaaaatcag ctcttggaga
     4801 taaagcatat gaatggaacg acaatgaaat gtacctgttc cgatcatctg ttgcatatgc
     4861 tatgaggcag tactttttaa aagtaaaaaa tcagatgatt ctttttgggg aggaggatgt
     4921 gcgagtggct aatttgaaac caagaatctc ctttaatttc tttgtcactg cacctaaaaa
     4981 tgtgtctgat atcattccta gaactgaagt tgaaaaggcc atcaggatgt cccggagccg
     5041 tatcaatgat gctttccgtc tgaatgacaa cagcctagag tttctgggga tacagccaac
     5101 acttggacct cctaaccagc cccctgtttc catatggctg attgtttttg gagttgtgat
     5161 gggagtgata gtggttggca ttgtcatcct gatcttcact gggatcagag atcggaagaa
     5221 gaaaaataaa gcaagaagtg gagaaaatcc ttatgcctcc atcgatatta gcaaaggaga
     5281 aaataatcca ggattccaaa acactgatga tgttcagacc tcctttggta ccgagacctc
     5341 ccaggtggcg cccgcttaat gtacaaaccg ctgttcctag gaatcccgag gcctctaccg
     5401 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     5461 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     5521 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     5581 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     5641 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     5701 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     5761 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     5821 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     5881 ctcatctccg ggcctttcgg acccgacatt agcgctacag cttaagcggg ccaccatgat
     5941 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6001 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6061 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     6121 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     6181 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     6241 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     6301 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     6361 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     6421 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     6481 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     6541 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     6601 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     6661 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     6721 gttcttctga ggatccgttt aaacgttgtt gtttggggtt gaattactct tctagaccgc
     6781 ggcgcgcccg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6841 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6901 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6961 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7021 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7081 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7141 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     7201 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7261 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7321 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7381 catcgatacc gtcgacctcg atcgagacct agaaaaacat ggagcaatca caagtagcaa
     7441 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     7501 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     7561 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     7621 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
     7681 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
     7741 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     7801 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     7861 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
     7921 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7981 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8041 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gcatgtgagc
     8101 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8161 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8221 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8281 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8341 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8401 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8461 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8521 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8581 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8641 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8701 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8761 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8821 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8881 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8941 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9001 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9061 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9121 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9181 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9241 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9301 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9361 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9421 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9481 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9541 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9601 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9661 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9721 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9781 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9841 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9901 tgacg
//
