LOCUS       sequence-367376-        4719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLCO4A1_STOP
FEATURES             Location/Qualifiers
     source          1..4719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2276..2375)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2392..2411)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2393..2411)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2416..2432)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2416..2432)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2534..2553)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2545..3354
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2612..2631)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3501..4089
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3990..4009
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4243..4260
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4419..4446)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4538..4624)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4673..4695
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4687..4704
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4688..4704
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgcc actgcaccag
      121 ctgggcgaca agcccctgac ctttcctagc ccaaactccg ccatggagaa tggcctggat
      181 cacaccccac cttctaggag agcaagccca ggaacaccac tgtctcctgg aagcctgcgg
      241 tccgccgcac actccccact ggacacctct aagcagcctc tgtgccagct gtgggcagag
      301 aagcacggag caaggggcac acacgaggtg agatacgtgt ccgccggaca gtctgtggca
      361 tgcggatggt gggccttcgc cccaccatgt ctgcaggtgc tgaacacccc caagggcatc
      421 ctgttcttcc tgtgcgcagc agccttcctg cagggaatga cagtgaacgg ctttatcaat
      481 accgtgatca caagcctgga gaggcgctac gacctgcact cttatcagag cggcctgatc
      541 gccagctcct atgatatcgc cgcctgcctg tgcctgacct tcgtgagcta ctttggagga
      601 tccggacaca agcctaggtg gctgggatgg ggcgtgctgc tgatgggaac cggatccctg
      661 gtgttcgcac tgccacactt tacagccggc aggtatgagg tggagctgga cgcaggcgtg
      721 cgcacctgcc cagcaaatcc aggagccgtg tgcgcagatt ccacatctgg cctgagccgc
      781 taccagctgg tgttcatgct gggacagttt ctgcacggag tgggagcaac accactgtac
      841 accctgggcg tgacatatct ggacgagaac gtgaagtcta gctgctcccc agtgtacatc
      901 gccatctttt ataccgcagc aatcctggga ccagcagcag gatatctgat cggaggcgcc
      961 ctgctgaata tctacaccga gatgggccgg agaacagagc tgaccacaga gtccccactg
     1021 tgggtgggag catggtgggt gggcttcctg ggatctggag cagcagcctt ctttacagca
     1081 gtgccaatcc tgggctatcc cagacagctg cctggcagcc agaggtacgc cgtgatgagg
     1141 gcagcagaga tgcaccagct gaaggattcc tctaggggag aggcaagcaa cccagacttc
     1201 ggcaagacca tccgcgatct gcctctgtcc atctggctgc tgctgaagaa tcccaccttc
     1261 atcctgctgt gcctggcagg agcaaccgag gccacactga tcaccggcat gtccaccttc
     1321 tctcctaagt ttctggagtc ccagttcagc ctgtccgcct ctgaggcagc caccctgttt
     1381 ggctatctgg tggtgccagc aggaggagga ggcacatttc tgggcggctt ctttgtgaac
     1441 aagctgaggc tgcgcggctc tgccgtgatc aagttctgcc tgttttgtac cgtggtgagc
     1501 ctgctgggca tcctggtgtt ctctctgcac tgccctagcg tgccaatggc aggagtgacc
     1561 gcaagctatg gaggatccct gctgcctgag ggacacctga acctgacagc cccatgcaat
     1621 gccgcctgct cttgtcagcc agagcactat tcccccgtgt gcggctctga cggcctgatg
     1681 tactttagcc tgtgccacgc aggatgtcca gcagcaaccg agacaaacgt ggacggacag
     1741 aaggtgtacc gggattgctc ctgtatccct cagaatctga gctccggatt cggacacgca
     1801 accgccggca agtgcacctc tacatgtcag agaaagcccc tgctgctggt gttcatcttt
     1861 gtggtcatct tcttcacatt cctgtctagc atccccgccc tgaccgccac actgagatgc
     1921 gtgagagatc ctcagcggag ctttgccctg ggcatccagt ggatcgtggt gagaatcctg
     1981 ggaggaatcc caggacctat cgccttcggc tgggtcatcg acaaggcctg tctgctgtgg
     2041 caggatcagt gcggccagca gggctcctgt ctggtgtacc agaatagcgc catgtccagg
     2101 tatatcctga tcatgggcct gctgtacaag gtgctgggcg tgctgttctt tgccatcgcc
     2161 tgcttcctgt acaagcctct gagcgagtcc tctgatggcc tggagacctg tctgccttcc
     2221 cagagctccg ccccagactc tgccacagat agccagctgc agtctagcgt gtaagaccca
     2281 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     2341 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     2401 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     2461 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     2521 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     2581 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     2641 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     2701 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     2761 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     2821 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     2881 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     2941 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     3001 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     3061 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     3121 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     3181 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     3241 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     3301 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     3361 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     3421 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     3481 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3541 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3601 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3661 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3721 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3781 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3841 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3901 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3961 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4021 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4081 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4141 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     4201 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     4261 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     4321 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     4381 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     4441 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     4501 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     4561 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     4621 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     4681 cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
