LOCUS       sequence-367381-        5102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter plasmid with a double-strand oligonucleotides that contain 
            five repeats of NF-kappaB binding consensus sequence 
            (5'-GGGGAATTTCC-3')..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Hernandez-Melchor D, Ramirez-Martinez L, Cid L, Palafox-Gomez C, 
            Lopez-Bayghen E, Ortega A
  TITLE     EAAT1-dependent slc1a3 Transcriptional Control depends on the 
            Substrate Translocation Process.
  JOURNAL   ASN Neuro. 2022 Jan-Dec;14:17590914221116574. doi: 
            10.1177/17590914221116574.
  PUBMED    35903937
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ASN Neuro. 
            2022 Jan-Dec;14:17590914221116574. doi: 10.1177/17590914221116574."
COMMENT     Sequence Label: pGL3Luc-5XNF-kappaB
FEATURES             Location/Qualifiers
     source          1..5102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        86..115
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     promoter        140..336
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      187..322
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      366..375
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             372..2024
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(438..456)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2065..2186
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2076..2095)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2130..2149
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2434..2451)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2605..3193)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2685..2704)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3364..4155,4156..4224))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3987..4006
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4225..4329)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4356..4811
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4443..4462)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4653..4674
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4942..4990
                     /note="synthetic polyadenylation signal"
     misc_feature    5004..5095
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5044..5063
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagccctc gaggggaatt tccggggact ttccgggaat
       61 ttccggggac tttccgggaa tttccagagg gtatataatg gaagctcgac ttccaaagct
      121 gggctcgaga tctgcgatct gcatctcaat tagtcagcaa ccatagtccc gcccctaact
      181 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tcgctgacta
      241 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
      301 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggcattcc ggtactgttg
      361 gtaaagccac catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc
      421 tggaagatgg aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc
      481 ctggaacaat tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact
      541 tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca
      601 gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat
      661 ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca
      721 gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt
      781 tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg
      841 attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta
      901 atgaatacga ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga
      961 actcctctgg atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg
     1021 tgagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga
     1081 ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga
     1141 tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc
     1201 ttcaggatta caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca
     1261 aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg
     1321 ctcccctctc taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca
     1381 ggcaaggata tgggctcact gagactacat cagctattct gattacaccc gagggggatg
     1441 ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg
     1501 ataccgggaa aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga
     1561 ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat
     1621 ggctacattc tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc
     1681 gcctgaagtc tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca
     1741 tcttgctcca acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg
     1801 ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag
     1861 agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg
     1921 tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag
     1981 agatcctcat aaaggccaag aagggcggaa agatcgccgt gtaattctag agtcggggcg
     2041 gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
     2101 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2161 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     2221 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa
     2281 tcgataagga tccgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     2341 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     2401 gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2461 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2521 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2581 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2641 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2701 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2761 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     2821 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2881 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2941 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3001 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     3061 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3121 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3181 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3241 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3301 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3361 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3421 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3481 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3541 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     3601 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     3661 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     3721 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     3781 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     3841 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     3901 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     3961 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4021 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4081 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4141 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4201 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4261 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4321 ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag
     4381 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4441 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4501 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4561 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4621 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4681 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4741 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4801 gcttacaatt tgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     4861 ggcctcttcg ctattacgcc agcccaagct accatgataa gtaagtaata ttaaggtacg
     4921 ggaggtactt ggagcggccg caataaaata tctttatttt cattacatct gtgtgttggt
     4981 tttttgtgtg aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac
     5041 aaactagcaa aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctctatcga
     5101 ta
//
