LOCUS       sequence-367383-        6383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 584, Emission: 607.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6383)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mRFP1-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..6383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        73..376
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        377..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     531..551
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      646..655
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2376..2385
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2382..2384,2385..2387,2388..3062)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(2565..2585)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2783..2802
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2977..3000
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    3183..3304
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3220..3239)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3274..3293
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3311..3766)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3448..3469)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3660..3679
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3793..3897
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3899..4256
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3899..4090
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3922..3942)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4107..4242
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4291..5085
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4291..5085
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4345..4364)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4955..4974
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5273..5292)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5317..5364
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5693..6281
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6182..6201
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc atagcccata
       61 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      121 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      181 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      241 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      301 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      361 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      421 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      481 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      541 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
      601 ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattccatg gcggcccccc
      661 gcccgtctcc cgcgatcccc gtttcggtct cggctccggc tttttacgcc ccgcagaaga
      721 agttcggccc tgtggtggcc ccaaagccca aagtgaatcc cttccggccc agggacagcg
      781 agcctccccc ggcacccggg gcccagcgcg cacagatggg ccgggtgggc gatattcccc
      841 cgccgccccc ggaagacttt cccctgcctc cacctcccct tgctggggat ggcgacgatg
      901 cagagggtgc tctgggaggt gccttcccgc cgccccctcc cccgatcgag gaatcatttc
      961 cccctgcgcc tctggaggag gagatcttcc cttccccgcc gcctcctccg gaggaggagg
     1021 gagggcctga ggcccccata ccgcccccac cacagcccag ggagaaggtg agcagtattg
     1081 atttggagat cgactctctg tcctcactgc tggatgacat gaccaagaat gatcctttca
     1141 aagcccgggt gtcatctgga tatgtgcccc caccagtggc cactccattc agttccaagt
     1201 ccagtaccaa gcctgcagcc gggggcacag cacccctgcc tccttggaag tccccttcca
     1261 gctcccagcc tctgccccag gttccggctc cggctcagag ccagacacag ttccatgttc
     1321 agccccagcc ccagcccaag cctcaggtcc aactccatgt ccagtcccag acccagcctg
     1381 tgtctttggc taacacccag ccccgagggc ccccagcctc atctccggct ccagccccta
     1441 agttttctcc agtgactcct aagtttactc ctgtggcttc caagttcagt cctggagccc
     1501 caggtggatc tgggtcacaa ccaaatcaaa aattggggca ccccgaagct ctttctgctg
     1561 gcacaggctc ccctcaacct cccagcttca cctatgccca gcagagggag aagccccgag
     1621 tgcaggagaa gcagcacccc gtgcccccac cggctcagaa ccaaaaccag gtgcgctccc
     1681 ctggggcccc agggcccctg actctgaagg aggtggagga gctggagcag ctgacccagc
     1741 agctaatgca ggacatggag catcctcaga ggcagaatgt ggctgtcaac gaactctgcg
     1801 gccgatgcca tcaacccctg gcccgggcgc agccagccgt ccgcgctcta gggcagctgt
     1861 tccacatcgc ctgcttcacc tgccaccagt gtgcgcagca gctccagggc cagcagttct
     1921 acagtctgga gggggcgccg tactgcgagg gctgttacac tgacaccctg gagaagtgta
     1981 acacctgcgg ggagcccatc actgaccgca tgctgagggc cacgggcaag gcctatcacc
     2041 cgcactgctt cacctgtgtg gtctgcgccc gccccctgga gggcacctcc ttcatcgtgg
     2101 accaggccaa ccggccccac tgtgtccccg actaccacaa gcagtacgcc ccgaggtgct
     2161 ccgtctgctc tgagcccatc atgcctgagc ctggccgaga tgagactgtg cgagtggtcg
     2221 ccctggacaa gaacttccac atgaagtgtt acaagtgtga ggactgcggg aagcccctgt
     2281 cgattgaggc agatgacaat ggctgcttcc ccctggacgg tcacgtgctc tgtcggaagt
     2341 gccacactgc tagagcccag acggatccac cggtcgccac catggtgcgc tcctccaaga
     2401 acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggcaccgtg aacggccacg
     2461 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggccacaac accgtgaagc
     2521 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttcc
     2581 agtacggctc caaggtgtac gtgaagcacc ccgccgacat ccccgactac aagaagctgt
     2641 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     2701 ccgtgaccca ggactcctcc ctgcaggacg gctgcttcat ctacaaggtg aagttcatcg
     2761 gcgtgaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     2821 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac aaggccctga
     2881 agctgaagga cggcggccac tacctggtgg agttcaagtc catctacatg gccaagaagc
     2941 ccgtgcagct gcccggctac tactacgtgg actccaagct ggacatcacc tcccacaacg
     3001 aggactacac catcgtggag cagtacgagc gcaccgaggg ccgccaccac ctgttcctgt
     3061 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     3121 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     3181 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3241 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3301 cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     3361 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     3421 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     3481 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     3541 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     3601 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     3661 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     3721 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
     3781 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3841 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct
     3901 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     3961 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     4021 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4081 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4141 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     4201 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc
     4261 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     4321 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     4381 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     4441 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     4501 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     4561 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     4621 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     4681 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     4741 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     4801 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     4861 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     4921 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     4981 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     5041 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     5101 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     5161 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     5221 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac
     5281 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
     5341 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     5401 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     5461 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     5521 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat
     5581 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5641 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5701 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5761 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5821 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5881 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5941 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6001 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6061 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6121 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6181 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6241 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6301 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6361 ctgattctgt ggataaccgt att
//
