LOCUS       sequence-36739-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 2314 (=HL 716 + pHL 818)
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    155..171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        229..333
                     /gene="bla"
                     /label=AmpR promoter
     CDS             518..896
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(552..571)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gggaagtctt ctccttncgc atgctagctg ggaccaggag ggacagtact ttaactttca
       61 tgttattaac cctctgttat atgcctttat ttgctttttt atgccactgc atactgatta
      121 accctcatta atcagtcggc aagtcgacag tatcttgtta tccgctcaca atgtcaatgt
      181 tatccgctca catttatgac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
      241 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      301 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      361 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
      421 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
      481 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
      541 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
      601 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
      661 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
      721 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
      781 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
      841 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
      901 aaactattaa ctggcgaact acttactcta gct
//
