LOCUS       sequence-36740-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Fan W, Nassiri A, Zhong Q
  TITLE     Autophagosome targeting and membrane curvature sensing by 
            Barkor/Atg14(L).
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7769-74. doi: 
            10.1073/pnas.1016472108. Epub 2011 Apr 25.
  PUBMED    21518905
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1016472108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-05-10- 10"; volume: "108"; issue: "19"; pages: "7769-74"
COMMENT     Sequence Label: Beclin-BATS
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             440..505
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(559..578)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(560..578)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(889..908)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ggatggatgt ggagaaggca agattgaaga cacaggaggc agtggcggct cctattccat
       61 caaaacccag tttaactctg aggagcagtg gacaaaagct ctcaagttca tgctgacgaa
      121 tcttaagtgg ggtcttgctt gggtgtcctc acaattttat aacaaagaag cttcgaattc
      181 ggctggagaa tcagatgaga gcggagatga gcgcgtcagc gatgaagaaa ccgacctggg
      241 cacagactgg gagaacttgc ctagtccccg gttttgtgat atcccttccc agtctgtgga
      301 agtctcccag agtcagagca cccaggcgtc cccacccatc gcgagcagca gtgcaggtgg
      361 gatgatctcc tctgcagcag cctcggtgac ctcctggttt aaagcttaca ctggacaccg
      421 ttcgaattct gcagtcgacg actacaaaga ccatgacggt gattataaag atcatgacat
      481 cgactacaag gatgacgatg acaagtaggg atccaccgga tctagataat agggtaccag
      541 gtaagtgtac ccaattcgcc ctatagtgag tcgtattaca attcactcga tcgcccttcc
      601 caacagttgc gcagcctgaa tggcgaatgg agatccaatt tttaagtgta taatgtgtta
      661 aactactgat tctaattgtt tgtgtatttt agattcacag tcccaaggct catttcaggc
      721 ccctcagtcc tcacagtctg ttcatgatca taatcagcca taccacattt gtagaggttt
      781 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
      841 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
      901 caaatttcac aaataaagca tttt
//
