LOCUS       sequence-367403-       10826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shApc-expressing lentiviruses with varius levels of expression of 
            SIINFEKL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10826)
  AUTHORS   Westcott PMK, Sacks NJ, Schenkel JM, Ely ZA, Smith O, Hauck H, 
            Jaeger AM, Zhang D, Backlund CM, Beytagh MC, Patten JJ, Elbashir R, 
            Eng G, Irvine DJ, Yilmaz OH, Jacks T
  TITLE     Low neoantigen expression and poor T-cell priming underlie early 
            immune escape in colorectal cancer.
  JOURNAL   Nat Cancer. 2021 Oct;2(10):1071-1085. doi: 
            10.1038/s43018-021-00247-z. Epub 2021 Sep 30.
  PUBMED    34738089
REFERENCE   2  (bases 1 to 10826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s43018-021-00247-z"; journalName: "Nat Cancer"; date: 
            "2021-10"; volume: "2"; issue: "10"; pages: "1071-1085"
COMMENT     Sequence Label: loSIIN
FEATURES             Location/Qualifiers
     source          1..10826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(19..38)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     265..285
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(294..722)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(404..425)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     616..635
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    768..992
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     981..998
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             1024..1257
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    1329..1917
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1435..1446
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1844..1864
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     ncRNA           complement(2017..2143)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(2171..2185)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(2213..2334)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     CDS             complement(join(2378..3091,3092..3094,3095..3097))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2381..3091,3092..3094,3095..3097))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2423..2444)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2772..2791
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3031..3052
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(3137..4315)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3146..4084
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(3169..3189)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4352..4363
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5055..5078
                     /codon_start=1
                     /product="antigenic CD8+ T cell epitope from chicken 
                     ovalbumin"
                     /label=OVA peptide
                     /translation="SIINFEKL"
     polyA_signal    5094..5228
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    5278..5395
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5693..5737)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5922..6155
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6648..6773
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6820..7000
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7018..7216)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7043..7063)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7218..7597
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7769..7788
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7863..7967
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7968..8036,8037..8828)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8186..8205)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8999..9587
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9488..9507
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9741..9758
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9875..9896
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9911..9916,9917..9934,9935..9941)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9949..9965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9954..9976
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9973..9989
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9973..9989
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    10038..10159
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(10289..10663)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(10682..10729)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(join(10777..10826,1..280))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10791..10826,1..100)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gctcagaggc agaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
       61 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga
      121 ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
      181 gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
      241 ctggggagcc tggggacttt ccacacccta actgacacac attccacaga attaattcgc
      301 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
      361 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
      421 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
      481 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
      541 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
      601 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
      661 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
      721 gtggggatac cccctagagc cccagctggt tctttccgcc tcagaagcca tagagcccac
      781 cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt cctgccccac
      841 cccacccccc agaatagaat gacacctact cagacaatgc gatgcaattt cctcatttta
      901 ttaggaaagg acagtgggag tggcaccttc cagggtcaag gaaggcacgg gggaggggca
      961 aacaacagat ggctggcaac tagaaggcac agtcgaggct gatcagcggg tttcgccggg
     1021 ccctgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt
     1081 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
     1141 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
     1201 acccagtaca agcaaaaagc agatcttgtc ttcgttggga gtgaattagc ccttccagtc
     1261 cccccttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta
     1321 aagtcgacgc ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag
     1381 acgcggaaga ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag
     1441 ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag
     1501 cagccaagga aaggacgatg atttccccga caacaccacg gaattgtcag tgcccaacag
     1561 ccgagcccct gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg
     1621 gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt
     1681 gggggttgcg tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa
     1741 ctcctcataa agagacagca accaggattt atacaaggag gagaaaatga aagccatacg
     1801 ggaagcaata gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc
     1861 aacatagtta agaataccag tcaatctttc acaaattttg taatccagag gttgattatc
     1921 gataagcttg atatcgaatt gtacctagtg gaaccggaac ccttaaacat gtagagtaat
     1981 tcaaccccaa acaaacaacg tttggtagaa ttgaaaaaag tgatttaatt tataccattt
     2041 taattcagct ttgtaaaaat gtatcaaaga gatagcaagg tattcagttt tagtaaacaa
     2101 gataattgct cctaaagtag ccccttgaat tccgaggcag taggcaacac caataaatta
     2161 cagtcttata catctgtggc ttcactataa gactgtaatt tattggtggc gctcactgtc
     2221 aacagcaata taccttctcg agccttctgt tgggttaacc tgaagaagta atcccagcaa
     2281 gtgtttccaa gatgtgcagg caacgattct gtaaagtact gaagcctcat tcaaacaatt
     2341 accctgttat ccctagtcga ggcttagccg tacgtcatta cttgtacagc tcgtccatgc
     2401 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
     2461 tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg
     2521 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct
     2581 cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga
     2641 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct
     2701 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
     2761 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt
     2821 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc
     2881 tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct
     2941 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
     3001 cgccagacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
     3061 ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggtcgaaaac gatcgcacag
     3121 ctagcgttcg agttagtcac gacacctgaa atggaagaaa aaaactttga accactgtct
     3181 gaggcttgag aatgaaccaa gatccaaact caaaaagggc aaattccaag gagaattaca
     3241 tcaagtgcca agctggccta acttcagtct ccacccactc agtgtgggga aactccatcg
     3301 cataaaaccc ctccccccaa cctaaagacg acgtactcca aaagctcgag aactaatcga
     3361 ggtgcctgga cggcgcccgg tactccgtgg agtcacatga agcgacggct gaggacggaa
     3421 aggccctttt cctttgtgtg ggtgactcac ccgcccgctc tcccgagcgc cgcgtcctcc
     3481 attttgagct ccctgcagca gggccgggaa gcggccatct ttccgctcac gcaactggtg
     3541 ccgaccgggc cagccttgcc gcccagggcg gggcgataca cggcggcgcg aggccaggca
     3601 ccagagcagg ccggccagct tgagactacc cccgtccgat tctcggtggc cgcgctcgca
     3661 ggccccgcct cgccgaacat gtgcgctggg acgcacgggc cccgtcgccg cccgcggccc
     3721 caaaaaccga aataccagtg tgcagatctt ggcccgcatt tacaagacta tcttgccaga
     3781 aaaaaagcgt cgcagcaggt catcaaaaat tttaaatggc tagagactta tcgaaagcag
     3841 cgagacaggc gcgaaggtgc caccagattc gcacgcggcg gccccagcgc ccaggccagg
     3901 cctcaactca agcacgaggc gaaggggctc cttaagcgca aggcctcgaa ctctcccacc
     3961 cacttccaac ccgaagctcg ggatcaagaa tcacgtactg cagccaggtg gaagtaattc
     4021 aaggcacgca agggccataa cccgtaaaga ggccaggccc gcgggaacca cacacggcac
     4081 ttacctgtgt tctggcggca aacccgttgc gaaaaagaac gttcacggcg actactgcac
     4141 ttatatacgg ttctccccca ccctcgggaa aaaggcggag ccagtacacg acatcacttt
     4201 cccagtttac cccgcgccac cttctctagg caccggttca attgccgacc cctcccccca
     4261 acttctcggg gactgtgggc gatgtgcgct ctgcccactg acgggcaccg gagccaaacc
     4321 agctttacac tccctcacac tgatcgatgg tatcgaaggg cgaggcagta ataaaggagt
     4381 tcatgcggtt caaggtacac atggagggct cgatgaacgg ccacgagttc gagatagagg
     4441 gcgagggcga gggccgcccc tacgagggca cccaaaccgc gaagttaaag gtaaccaagg
     4501 gtggcccctt acccttctcg tgggacatac tgtcgcctca attcatgtac ggctcgaggg
     4561 cgttcaccaa gcaccccgcg gacatacccg actactataa gcaatcgttc cccgagggct
     4621 tcaagtggga gcgcgtaatg aacttcgagg acggcggcgc ggtaaccgta acccaagaca
     4681 cctcgttaga ggacggcacc ttaatataca aggtaaagtt acgcggcacc aacttccctc
     4741 ctgacggccc cgtaatgcaa aagaagacaa tgggctggga agcgtcgacc gagcggttgt
     4801 accccgagga cggcgtatta aagggcgaca ttaagatggc gttacgctta aaggacggcg
     4861 gccgctactt agcggacttc aagaccacct acaaggcgaa gaagcccgta caaatgcccg
     4921 gcgcgtacaa cgtcgaccgc aagttggaca taacctcgca caacgaggac tacaccgtag
     4981 tagaacaata cgaacgctcg gagggccgcc actcgaccgg cggcatggac gagttataca
     5041 aggagcaatt agagagtata atcaactttg aaaaactgta atgcgacgga tccaacttgt
     5101 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5161 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5221 tctggatcgt accttaatta accaaactgg atctctgctg tccctgtaat aaacccgaaa
     5281 attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
     5341 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg
     5401 gatgaatact gccatttgtc tgcagaattg gcgcacgcag tgccgatccg ttcactaatc
     5461 gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg
     5521 gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc
     5581 tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca
     5641 ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg
     5701 cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt
     5761 caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg
     5821 tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag
     5881 cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta
     5941 ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt
     6001 gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac
     6061 cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc
     6121 ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct
     6181 gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt
     6241 tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca ggtctgaaga
     6301 tcagcggccg cttgctgtgc ggtggtctta cttttgtttt gctcttcctc tatcttgtct
     6361 aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact
     6421 gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc
     6481 agtatttgtc tacagccttc tgatgtttct aacaggccag gattaactgc gaatcgttct
     6541 agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt
     6601 aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac
     6661 ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc
     6721 ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gagctcctct
     6781 ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg
     6841 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     6901 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     6961 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaggca aaacgcgctg
     7021 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
     7081 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
     7141 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
     7201 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     7261 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     7321 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     7381 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     7441 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     7501 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     7561 attgattact attaataact agtcaataat caatgtcaac gcgtatatct ggcccgtaca
     7621 tcgcgaagca gcgcaaaacg cctaacccta agcagattct tcatgcaatt gtcggtcaag
     7681 ccttgccttg ttgtagctta aattttgctc gcgcactact cagcgacctc caacacacaa
     7741 gcagggagca gatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     7801 cataggggat cgggagatct cccgatccgt cgacgtcagg tggcactttt cggggaaatg
     7861 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7921 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7981 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8041 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8101 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8161 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8221 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8281 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8341 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8401 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8461 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8521 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8581 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8641 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8701 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8761 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8821 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8881 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8941 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9001 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9061 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9121 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9181 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9241 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9301 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9361 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9421 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9481 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9541 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9601 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9661 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9721 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     9781 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     9841 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     9901 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     9961 aacaatttca cacaggaaac agctatgacc atgattacgc caagctctag ctagaggtcg
    10021 acggtataca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
    10081 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
    10141 aagctgcaat aaacaagttg gggtgggcga agaactccag catgagatcc ccgcgctgga
    10201 ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg
    10261 cggtggaatc gaaatctcgt agcacgtgtc agtcctgctc ctcggccacg aagtgcacgc
    10321 agttgccggc cgggtcgcgc agggcgaact cccgccccca cggctgctcg ccgatctcgg
    10381 tcatggccgg cccggaggcg tcccggaagt tcgtggacac gacctccgac cactcggcgt
    10441 acagctcgtc caggccgcgc acccacaccc aggccagggt gttgtccggc accacctggt
    10501 cctggaccgc gctgatgaac agggtcacgt cgtcccggac cacaccggcg aagtcgtcct
    10561 ccacgaagtc ccgggagaac ccgagccggt cggtccagaa ctcgaccgct ccggcgacgt
    10621 cgcgcgcggt gagcaccgga acggcactgg tcaacttggc catggtttag ttcctcacct
    10681 tgtcgtatta tactatgccg atatactatg ccgatgatta attgtcaaca cgtgctgatc
    10741 agatccgaaa atggatatac aagctcccgg gagctttttg caaaagccta ggcctccaaa
    10801 aaagcctcct cactacttct ggaata
//
