LOCUS       sequence-367404-        9950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shApc-expressing lentiviruses with varius levels of expression of 
            SIINFEKL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Westcott PMK, Sacks NJ, Schenkel JM, Ely ZA, Smith O, Hauck H, 
            Jaeger AM, Zhang D, Backlund CM, Beytagh MC, Patten JJ, Elbashir R, 
            Eng G, Irvine DJ, Yilmaz OH, Jacks T
  TITLE     Low neoantigen expression and poor T-cell priming underlie early 
            immune escape in colorectal cancer.
  JOURNAL   Nat Cancer. 2021 Oct;2(10):1071-1085. doi: 
            10.1038/s43018-021-00247-z. Epub 2021 Sep 30.
  PUBMED    34738089
REFERENCE   2  (bases 1 to 9950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s43018-021-00247-z"; journalName: "Nat Cancer"; date: 
            "2021-10"; volume: "2"; issue: "10"; pages: "1071-1085"
COMMENT     Sequence Label: medSIIN
FEATURES             Location/Qualifiers
     source          1..9950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        215..413
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     368..388
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             431..611
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    658..783
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1276..1509
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1694..1738
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2036..2153
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2234..3412
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2465..3403
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3360..3380
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3443..4138
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(3576..3594)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3844..3863
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4151..4174
                     /codon_start=1
                     /product="antigenic CD8+ T cell epitope from chicken 
                     ovalbumin"
                     /label=OVA peptide
                     /translation="SIINFEKL"
     ncRNA           4221..4342
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           4370..4384
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           4412..4538
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     misc_feature    4638..5226
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4691..4711)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5109..5120)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5298..5531
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5557..5574)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5563..5787
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5833..6261
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5920..5939)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6130..6151
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6270..6290)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6275..6604
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6455..6590
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6517..6536
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6652..6699
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6718..7092
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7222..7343
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7259..7278)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7313..7332
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7392..7408)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7392..7408)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7405..7427)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7416..7432
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7440..7446,7447..7464,7465..7470))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7485..7506
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7623..7640)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7794..8382)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7874..7893)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8553..9344,9345..9413))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9176..9195
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9414..9518)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9593..9612)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9784..9950,1..213)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
       61 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      121 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      181 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      241 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      301 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      361 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
      421 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      481 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
      541 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      601 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
      661 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
      721 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
      781 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
      841 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
      901 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
      961 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     1021 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     1081 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     1141 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     1201 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     1261 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     1321 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     1381 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     1441 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     1501 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     1561 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     1621 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     1681 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     1741 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     1801 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     1861 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     1921 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     1981 cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta
     2041 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     2101 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     2161 attacaggga cagcagagat ccagtttggt taattaaggt accgatcagt gtgagggagt
     2221 gtaaagctgg tttggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     2281 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     2341 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     2401 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     2461 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     2521 cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     2581 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     2641 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     2701 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     2761 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     2821 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     2881 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     2941 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     3001 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     3061 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     3121 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     3181 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     3241 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     3301 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     3361 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gactaactcg
     3421 aacgctagct gtgcgatcgt ttatggtgag caagggcgag gcagtgatca aggagttcat
     3481 gcggttcaag gtgcacatgg agggctccat gaacggccac gagttcgaga tcgagggcga
     3541 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     3601 ccccctgccc ttctcctggg acatcctgtc ccctcagttc atgtacggct ccagggcctt
     3661 caccaagcac cccgccgaca tccccgacta ctataagcag tccttccccg agggcttcaa
     3721 gtgggagcgc gtgatgaact tcgaggacgg cggcgccgtg accgtgaccc aggacacctc
     3781 cctggaggac ggcaccctga tctacaaggt gaagctccgc ggcaccaact tccctcctga
     3841 cggccccgta atgcagaaga agacaatggg ctgggaagcg tccaccgagc ggttgtaccc
     3901 cgaggacggc gtgctgaagg gcgacattaa gatggccctg cgcctgaagg acggcggccg
     3961 ctacctggcg gacttcaaga ccacctacaa ggccaagaag cccgtgcaga tgcccggcgc
     4021 ctacaacgtc gaccgcaagt tggacatcac ctcccacaac gaggactaca ccgtggtgga
     4081 acagtacgaa cgctccgagg gccgccactc caccggcggc atggacgagc tgtacaagga
     4141 gcagctcgag agtataatca actttgaaaa actgtaatga cgtacggcta agcctcgact
     4201 agggataaca gggtaattgt ttgaatgagg cttcagtact ttacagaatc gttgcctgca
     4261 catcttggaa acacttgctg ggattacttc ttcaggttaa cccaacagaa ggctcgagaa
     4321 ggtatattgc tgttgacagt gagcgccacc aataaattac agtcttatag tgaagccaca
     4381 gatgtataag actgtaattt attggtgttg cctactgcct cggaattcaa ggggctactt
     4441 taggagcaat tatcttgttt actaaaactg aataccttgc tatctctttg atacattttt
     4501 acaaagctga attaaaatgg tataaattaa atcacttttt tcaattctac caaacgttgt
     4561 ttgtttgggg ttgaattact ctacatgttt aagggttccg gttccactag gtacaattcg
     4621 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4681 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4741 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     4801 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     4861 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     4921 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     4981 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     5041 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5101 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5161 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5221 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     5281 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     5341 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     5401 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     5461 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     5521 aatctctagc agggcccggc gaaacccgct gatcagcctc gactgtgcct tctagttgcc
     5581 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     5641 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     5701 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     5761 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     5821 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     5881 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     5941 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6001 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     6061 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6121 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     6181 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6241 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     6301 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     6361 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6421 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6481 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6541 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     6601 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     6661 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     6721 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
     6781 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
     6841 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
     6901 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
     6961 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
     7021 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
     7081 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     7141 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     7201 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     7261 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7321 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     7381 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7441 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7501 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     7561 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7621 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7681 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7741 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7801 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7861 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7921 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7981 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8041 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8101 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8161 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8221 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8281 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8341 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8401 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8461 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8521 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8581 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8641 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8701 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8761 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8821 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8881 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8941 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9001 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9061 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9121 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9181 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9241 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9301 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9361 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9421 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9481 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9541 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     9601 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     9661 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     9721 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     9781 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     9841 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     9901 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
//
