LOCUS       sequence-367405-        9953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shApc-expressing lentiviruses with varius levels of expression of 
            SIINFEKL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9953)
  AUTHORS   Westcott PMK, Sacks NJ, Schenkel JM, Ely ZA, Smith O, Hauck H, 
            Jaeger AM, Zhang D, Backlund CM, Beytagh MC, Patten JJ, Elbashir R, 
            Eng G, Irvine DJ, Yilmaz OH, Jacks T
  TITLE     Low neoantigen expression and poor T-cell priming underlie early 
            immune escape in colorectal cancer.
  JOURNAL   Nat Cancer. 2021 Oct;2(10):1071-1085. doi: 
            10.1038/s43018-021-00247-z. Epub 2021 Sep 30.
  PUBMED    34738089
REFERENCE   2  (bases 1 to 9953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s43018-021-00247-z"; journalName: "Nat Cancer"; date: 
            "2021-10"; volume: "2"; issue: "10"; pages: "1071-1085"
COMMENT     Sequence Label: midSIIN
FEATURES             Location/Qualifiers
     source          1..9953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      140..568
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(227..246)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     437..458
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(577..597)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        582..911
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      762..897
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     824..843
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        959..1006
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1025..1399
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1529..1650
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1566..1585)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1620..1639
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1699..1715)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1699..1715)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1712..1734)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1723..1739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1747..1753,1754..1771,1772..1777))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1792..1813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1930..1947)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2101..2689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2181..2200)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2860..3651,3652..3720))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3483..3502
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3721..3825)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3900..3919)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4091..4470
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4472..4670
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4625..4645
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4688..4868
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4915..5040
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5533..5766
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5951..5995
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6293..6410
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6491..7669
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6722..7660
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7617..7637
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7705..7716
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             8408..8431
                     /codon_start=1
                     /product="antigenic CD8+ T cell epitope from chicken 
                     ovalbumin"
                     /label=OVA peptide
                     /translation="SIINFEKL"
     ncRNA           8481..8602
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           8630..8644
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           8672..8798
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     misc_feature    8898..9486
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8951..8971)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9369..9380)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9558..9791
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9817..9834)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    join(9823..9953,1..94)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
       61 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
      121 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      181 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      241 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
      301 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
      361 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
      421 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
      481 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
      541 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
      601 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      661 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
      721 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
      781 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
      841 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
      901 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
      961 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     1021 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     1081 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     1141 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     1201 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     1261 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     1321 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     1381 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
     1441 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     1501 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     1561 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     1621 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     1681 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     1741 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     1801 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     1861 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     1921 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1981 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2041 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2101 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2161 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2221 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2281 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2341 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2401 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2461 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2521 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2581 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2641 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2701 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2761 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2821 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2881 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2941 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3001 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3061 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3121 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3181 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3241 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3301 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3361 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3421 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3481 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3541 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3601 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3661 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3721 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3781 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3841 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     3901 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     3961 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     4021 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     4081 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     4141 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     4201 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     4261 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     4321 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     4381 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     4441 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     4501 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     4561 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     4621 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
     4681 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     4741 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     4801 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     4861 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     4921 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     4981 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     5041 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     5101 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     5161 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     5221 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     5281 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     5341 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     5401 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     5461 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     5521 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     5581 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     5641 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     5701 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     5761 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     5821 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     5881 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     5941 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     6001 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     6061 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     6121 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     6181 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     6241 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     6301 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     6361 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     6421 acagggacag cagagatcca gtttggttaa ttaaggtacc gatcagtgtg agggagtgta
     6481 aagctggttt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     6541 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     6601 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     6661 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     6721 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     6781 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
     6841 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
     6901 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
     6961 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
     7021 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
     7081 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
     7141 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     7201 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     7261 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     7321 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     7381 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     7441 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     7501 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     7561 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     7621 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgac taactcgaac
     7681 gctagctgtg cgatcgttta tggtatcgaa gggcgaggca gtaataaagg agttcatgcg
     7741 gttcaaggta cacatggagg gctcgatgaa cggccacgag ttcgagatag agggcgaggg
     7801 cgagggccgc ccctacgagg gcacccaaac cgcgaagtta aaggtaacca agggtggccc
     7861 cttacccttc tcgtgggaca tactgtcgcc tcaattcatg tacggctcga gggcgttcac
     7921 caagcacccc gcggacatac ccgactacta taagcaatcg ttccccgagg gcttcaagtg
     7981 ggagcgcgta atgaacttcg aggacggcgg cgcggtaacc gtaacccaag acacctcgtt
     8041 agaggacggc accttaatat acaaggtaaa gttacgcggc accaacttcc ctcctgacgg
     8101 ccccgtaatg caaaagaaga caatgggctg ggaagcgtcg accgagcggt tgtaccccga
     8161 ggacggcgta ttaaagggcg acattaagat ggcgttacgc ttaaaggacg gcggccgcta
     8221 cttagcggac ttcaagacca cctacaaggc gaagaagccc gtacaaatgc ccggcgcgta
     8281 caacgtcgac cgcaagttgg acataacctc gcacaacgag gactacaccg tagtagaaca
     8341 atacgaacgc tcggagggcc gccactcgac cggcggcatg gacgagttat acaaggagca
     8401 attagagagt ataatcaact ttgaaaaact gtaatgctga cgtacggcta agcctcgact
     8461 agggataaca gggtaattgt ttgaatgagg cttcagtact ttacagaatc gttgcctgca
     8521 catcttggaa acacttgctg ggattacttc ttcaggttaa cccaacagaa ggctcgagaa
     8581 ggtatattgc tgttgacagt gagcgccacc aataaattac agtcttatag tgaagccaca
     8641 gatgtataag actgtaattt attggtgttg cctactgcct cggaattcaa ggggctactt
     8701 taggagcaat tatcttgttt actaaaactg aataccttgc tatctctttg atacattttt
     8761 acaaagctga attaaaatgg tataaattaa atcacttttt tcaattctac caaacgttgt
     8821 ttgtttgggg ttgaattact ctacatgttt aagggttccg gttccactag gtacaattcg
     8881 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     8941 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     9001 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     9061 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     9121 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     9181 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     9241 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     9301 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     9361 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     9421 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     9481 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     9541 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     9601 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     9661 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     9721 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     9781 aatctctagc agggcccggc gaaacccgct gatcagcctc gactgtgcct tctagttgcc
     9841 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     9901 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgt
//
