LOCUS       sequence-367412-        8202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of dHaloTag7 located at the intermediate filaments of 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Kompa J, Bruins J, Glogger M, Wilhelm J, Frei MS, Tarnawski M, 
            D'Este E, Heilemann M, Hiblot J, Johnsson K
  TITLE     Exchangeable HaloTag Ligands for Super-Resolution Fluorescence 
            Microscopy.
  JOURNAL   J Am Chem Soc. 2023 Feb 8;145(5):3075-3083. doi: 
            10.1021/jacs.2c11969. Epub 2023 Jan 30.
  PUBMED    36716211
REFERENCE   2  (bases 1 to 8202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.2c11969"; journalName: "J Am Chem Soc"; date: 
            "2023-02-8- 8"; volume: "145"; issue: "5"; pages: "3075-3083"
COMMENT     Sequence Label: pCDNA5/FRT/TO_Vimentin-dHaloTag7_T2A_mEGFP
FEATURES             Location/Qualifiers
     source          1..8202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2468..3352
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHA
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             3353..3406
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3407..3409,3410..4123)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3407..3409,3410..4120)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3449..3470)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3710..3729)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4057..4078
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4155..4172)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4161..4385
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4518..4537)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4669..4716
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4724..5746
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4724..5746
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5876..5997
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5913..5932)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5967..5986
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6046..6062)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6046..6062)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6059..6081)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6070..6086
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6094..6100,6101..6118,6119..6124))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6139..6160
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6277..6294)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6448..7035)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6527..6546)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7206..7997,7998..8066))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7829..7848
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8067..8171)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga
     1021 attctgcaga tatccagcaa ccatgtccac caggtccgtg tcctcgtcct cctaccgcag
     1081 gatgttcggc ggcccgggca ccgcgagccg gccgagctcc agccggagct acgtgactac
     1141 gtccacccgc acctacagcc tgggcagcgc gctgcgcccc agcaccagcc gcagcctcta
     1201 cgcctcgtcc ccgggcggcg tgtatgccac gcgctcctct gccgtgcgcc tgcggagcag
     1261 cgtgcccggg gtgcggctcc tgcaggactc ggtggacttc tcgctggccg acgccatcaa
     1321 caccgagttc aagaacaccc gcaccaacga gaaggtggag ctgcaggagc tgaatgaccg
     1381 cttcgccaac tacatcgaca aggtgcgctt cctggagcag cagaataaga tcctgctggc
     1441 cgagctcgag cagctcaagg gccaaggcaa gtcgcgcctg ggggacctct acgaggagga
     1501 gatgcgggag ctgcgccggc aggtggacca gctaaccaac gacaaagccc gcgtcgaggt
     1561 ggagcgcgac aacctggccg aggacatcat gcgcctccgg gagaaattgc aggaggagat
     1621 gcttcagaga gaggaagccg aaaacaccct gcaatctttc agacaggatg ttgacaatgc
     1681 gtctctggca cgtcttgacc ttgaacgcaa agtggaatct ttgcaagaag agattgcctt
     1741 tttgaagaaa ctccacgaag aggaaatcca ggagctgcag gctcagattc aggaacagca
     1801 tgtccaaatc gatgtggatg tttccaagcc tgacctcacg gctgccctgc gtgacgtacg
     1861 tcagcaatat gaaagtgtgg ctgccaagaa cctgcaggag gcagaagaat ggtacaaatc
     1921 caagtttgct gacctctctg aggctgccaa ccggaacaat gacgccctgc gccaggcaaa
     1981 gcaggagtcc actgagtacc ggagacaggt gcagtccctc acctgtgaag tggatgccct
     2041 taaaggaacc aatgagtccc tggaacgcca gatgcgtgaa atggaagaga actttgccgt
     2101 tgaagctgct aactaccaag acactattgg ccgcctgcag gatgagattc agaatatgaa
     2161 ggaggaaatg gctcgtcacc ttcgtgaata ccaagacctg ctcaatgtta agatggccct
     2221 tgacattgag attgccacct acaggaagct gctggaaggc gaggagagca ggatttctct
     2281 gcctcttcca aacttttcct ccctgaacct gagggaaact aatctggatt cactccctct
     2341 ggttgatacc cactcaaaaa ggacacttct gattaagacg gttgaaacta gagatggaca
     2401 ggttatcaac gaaacttctc agcatcacga tgaccttgaa ggggatccac cggtcgccac
     2461 cggatccgaa atcggtactg gctttccatt cgacccccat tatgtggaag tcctgggcga
     2521 gcgcatgcac tacgtcgatg ttggtccgcg cgatggcacc cctgtgctgt tcctgcacgg
     2581 taacccgacc tcctcctacg tgtggcgcaa catcatcccg catgttgcac cgacccatcg
     2641 ctgcattgct ccagacctga tcggtatggg caaatccgac aaaccagacc tgggttattt
     2701 cttcgacgac cacgtccgct tcatggatgc cttcatcgaa gccctgggtc tggaagaggt
     2761 cgtcctggtc attcacgcct ggggctccgc tctgggtttc cactgggcca agcgcaatcc
     2821 agagcgcgtc aaaggtattg catttatgga gttcatccgc cctatcccga cctgggacga
     2881 atggccagaa tttgcccgcg agaccttcca ggccttccgc accaccgacg tcggccgcaa
     2941 gctgatcatc gatcagaacg tttttatcga gggtacgctg ccgatgggtg tcgtccgccc
     3001 gctgactgaa gtcgagatgg accattaccg cgagccgttc ctgaatcctg ttgaccgcga
     3061 gccactgtgg cgcttcccaa acgagctgcc aatcgccggt gagccagcga acatcgtcgc
     3121 gctggtcgaa gaatacatgg actggctgca ccagtcccct gtcccgaagc tgctgttctg
     3181 gggcacccca ggcgttctga tcccaccggc cgaagccgct cgcctggcca aaagcctgcc
     3241 taactgcaag gctgtggaca tcggcccggg tctgaatctg ctgcaagaag acaacccgga
     3301 cctgatcggc agcgagatcg cgcgctggct gtctactctg gagatttccg gtgagggcag
     3361 aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct ggcccagtga gcaagggcga
     3421 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     3481 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     3541 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     3601 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     3661 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     3721 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     3781 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     3841 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     3901 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3961 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     4021 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     4081 cgccgccggg atcactctcg gcatggacga gctgtacaag tgatctagag ggcccgttta
     4141 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4201 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4261 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4321 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4381 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     4441 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4501 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4561 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4621 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtacctaga agttcctatt
     4681 ccgaagttcc tattctctag aaagtatagg aacttccttg gccaaaaagc ctgaactcac
     4741 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     4801 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     4861 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     4921 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
     4981 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     5041 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
     5101 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     5161 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     5221 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     5281 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     5341 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     5401 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     5461 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     5521 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     5581 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     5641 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     5701 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagcacg tactacgaga
     5761 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     5821 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     5881 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5941 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6001 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     6061 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6121 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6181 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6241 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6301 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6361 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6421 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgccccctga cgagcatcac
     6481 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6541 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6601 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6661 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6721 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6781 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6841 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6901 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6961 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7021 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7081 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7141 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7201 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7261 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7321 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7381 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7441 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7501 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7561 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7621 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7681 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7741 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7801 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7861 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7921 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7981 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8041 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8101 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8161 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
