LOCUS       sequence-36742-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 2817 (=HL 716 + pHL 720)
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    238..254
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        312..416
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(635..654)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcgggaagtc ttctccttta cgcatgctcg cggtaccttt aatcaggttg ttcaggttag
       61 tgacataagt ctgatggtgc ttgccgtagt gatactcgat ggtttccgca gaaatgtgcg
      121 gtgccagagc atctttagca tatggtagtg caggtaattc gaatgacatt gctactctcc
      181 tttattatta atttgcatac gtacaatagc cttattgtgc gtatggtcga cagtatcttg
      241 ttatccgctc acaatgtcaa tgttatccgc tcacatttat gacgtcaggt ggcacttttc
      301 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      361 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
      421 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      481 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      541 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      601 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
      661 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      721 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
      781 gtgctgccat aaccatgagt gatgacactg c
//
