LOCUS       sequence-367452-        6297 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector encoding C-terminal portion of split-Intein-mediated 
            split-Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Kim S, Kim YE, Song I, Ujihara Y, Kim N, Jiang YH, Yin HH, Lee TH, 
            Kim IH
  TITLE     Neural circuit pathology driven by Shank3 mutation disrupts social 
            behaviors.
  JOURNAL   Cell Rep. 2022 Jun 7;39(10):110906. doi: 
            10.1016/j.celrep.2022.110906.
  PUBMED    35675770
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-06-7"; volume: "39(10):110906. doi"; pages: " 
            10.1016/j.celrep.2022.110906"
COMMENT     Sequence Label: AAV-Ef1a-InteinC-CreC
FEATURES             Location/Qualifiers
     source          1..6297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   25..154
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        255..1433
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          486..1424
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1381..1401
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2448..3036
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2501..2521)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2919..2930)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3068..3544
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3267..3286)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3584..3724
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3584..3713
                     /label=AAV2 ITR
     rep_origin      3799..4254
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3886..3905)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4096..4117
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4271..4290)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4390..4412
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4450..4468)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4536..4640
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4641..4709,4710..5501)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4859..4878)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5672..6260
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6161..6180
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttgctggcc ttttgctcac atgtcctgca ggcagctgcg cgctcgctcg ctcactgagg
       61 ccgcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga
      121 gagggagtgg ccaactccat cactaggggt tcctgcggcc gcacgcgtaa gctttgcaaa
      181 gatggataaa gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga
      241 aaggagtggg aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
      301 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg
      361 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
      421 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
      481 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt
      541 gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg
      601 ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt
      661 gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
      721 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
      781 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt
      841 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
      901 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
      961 ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
     1021 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga
     1081 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
     1141 aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt
     1201 ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg
     1261 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt
     1321 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc
     1381 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggtaccg
     1441 gatctatggt taaagttatc ggtcgtcgtt ccctcggagt gcaaagaata tttgatattg
     1501 gtcttcccca agaccataat tttctgctag ccaatggggc gatcgccgcc aattgtttta
     1561 acaaatccaa ccggaaatgg tttcccgcag aacctgaaga tgttcgcgat tatcttctat
     1621 atcttcaggc gcgcggtctg gcagtaaaaa ctatccagca acatttgggc cagctaaaca
     1681 tgcttcatcg tcggtccggg ctgccacgac caagtgacag caatgctgtt tcactggtta
     1741 tgcggcggat ccgaaaagaa aacgttgatg ccggtgaacg tgcaaaacag gctctagcgt
     1801 tcgaacgcac tgatttcgac caggttcgtt cactcatgga aaatagcgat cgctgccagg
     1861 atatacgtaa tctggcattt ctggggattg cttataacac cctgttacgt atagccgaaa
     1921 ttgccaggat cagggttaaa gatatctcac gtactgacgg tgggagaatg ttaatccata
     1981 ttggcagaac gaaaacgctg gttagcaccg caggtgtaga gaaggcactt agcctggggg
     2041 taactaaact ggtcgagcga tggatttccg tctctggtgt agctgatgat ccgaataact
     2101 acctgttttg ccgggtcaga aaaaatggtg ttgccgcgcc atctgccacc agccagctat
     2161 caactcgcgc cctggaaggg atttttgaag caactcatcg attgatttac ggcgctaagg
     2221 atgactctgg tcagagatac ctggcctggt ctggacacag tgcccgtgtc ggagccgcgc
     2281 gagatatggc ccgcgctgga gtttcaatac cggagatcat gcaagctggt ggctggacca
     2341 atgtaaatat tgtcatgaac tatatccgta acctggatag tgaaacaggg gcaatggtgc
     2401 gcctgctgga agatggcgat taggaattcg atatcaagct tatcgataat caacctctgg
     2461 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     2521 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     2581 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     2641 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     2701 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     2761 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     2821 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca
     2881 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     2941 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     3001 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga
     3061 gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc
     3121 cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta
     3181 ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg
     3241 gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca
     3301 atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc
     3361 cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta
     3421 gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta
     3481 cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt
     3541 ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg
     3601 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     3661 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     3721 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     3781 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3841 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
     3901 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     3961 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     4021 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4081 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
     4141 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
     4201 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     4261 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     4321 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     4381 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     4441 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     4501 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4561 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4621 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4681 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4741 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4801 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4861 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4921 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4981 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5041 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5101 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5161 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5221 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5281 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5341 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5401 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5461 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5521 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5581 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5641 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5701 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5761 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5821 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5881 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5941 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6001 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6061 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6121 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6181 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6241 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcct
//
