LOCUS       sequence-367454-       13363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral vector expressing RFP670 alongside Cas9 and an sgRNA 
            cloning site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13363)
  TITLE     CRISPR and tetop plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LentiCRISPRv2 RFP670
FEATURES             Location/Qualifiers
     source          1..13363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..42)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(196..784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(276..295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(955..1746,1747..1815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1578..1597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1816..1920)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1995..2014)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2186..2565
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2567..2765
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2720..2740
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2783..2963
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3010..3135
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3628..3861
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4046..4090
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4388..4505
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4556..4804
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4556..4576
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4727..4746
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4821..4896
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        4958..5169
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      5188..5197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5194..9297
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5194..9297
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9298..9345
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9346..9369
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9385..9441
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    10403..10991
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10456..10476)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10874..10885)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11063..11296
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(11322..11339)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11328..11552
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11598..12026
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11685..11704)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11895..11916
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12035..12055)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12040..12369
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12220..12355
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12282..12301
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12417..12464
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12483..12857
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12987..13108
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13024..13043)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13078..13097
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13157..13173)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13157..13173)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13170..13192)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13181..13197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13205..13211,13212..13229,13230..13235))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13250..13271
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
       61 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      841 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
      901 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
      961 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1021 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1081 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1141 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1201 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1261 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1321 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1381 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     1441 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     1501 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     1561 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     1621 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     1681 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     1741 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     1801 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     1861 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     1921 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     1981 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     2041 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     2101 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     2161 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     2221 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     2281 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     2341 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     2401 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     2461 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     2521 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     2581 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     2641 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     2701 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     2761 agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     2821 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     2881 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     2941 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     3001 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     3061 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     3121 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     3181 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     3241 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     3301 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     3361 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     3421 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     3481 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     3541 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     3601 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     3661 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     3721 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     3781 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     3841 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     3901 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     3961 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     4021 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     4081 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     4141 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     4201 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     4261 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     4321 tcgattagtg aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca
     4381 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     4441 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     4501 attttcgggt ttattacagg gacagcagag atccagtttg gttaattaag gtaccgaggg
     4561 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
     4621 tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
     4681 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
     4741 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
     4801 caccggagac ggacgtctct gttttagagc tagaaatagc aagttaaaat aaggctagtc
     4861 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc tagctaggtc
     4921 ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     4981 gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag aaggtggcgc
     5041 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     5101 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     5161 agaacacagg accggttcta gagcgctgcc accatggaca agaagtacag catcggcctg
     5221 gacatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc
     5281 aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga
     5341 gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga
     5401 agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag
     5461 atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag
     5521 gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac
     5581 gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc
     5641 gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg
     5701 atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg
     5761 cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag
     5821 gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg
     5881 cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc
     5941 cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac
     6001 acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg
     6061 tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac
     6121 accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac
     6181 caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag
     6241 attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag
     6301 gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg
     6361 ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc
     6421 atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt
     6481 tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc
     6541 tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc
     6601 gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag
     6661 agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc
     6721 aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac
     6781 gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg
     6841 gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc
     6901 aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc
     6961 tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat
     7021 gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga
     7081 gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag
     7141 cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc
     7201 atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc
     7261 aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag
     7321 aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc
     7381 agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa
     7441 gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc
     7501 acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa
     7561 gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag
     7621 aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac
     7681 atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac
     7741 gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac
     7801 gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc
     7861 aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc
     7921 gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag
     7981 cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg
     8041 atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat
     8101 ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg
     8161 aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg
     8221 tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc
     8281 ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag
     8341 attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc
     8401 ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg
     8461 ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct
     8521 atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag
     8581 aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg
     8641 gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg
     8701 gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa
     8761 gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc
     8821 cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc
     8881 tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc
     8941 gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc
     9001 atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa
     9061 gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc
     9121 atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc
     9181 accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac
     9241 cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggcgacaag
     9301 cgacctgccg ccacaaagaa ggctggacag gctaagaaga agaaagatta caaagacgat
     9361 gacgataagg gatccggatc tggtgccacg aatttctcac tactcaagca ggccggtgat
     9421 gtcgaggaaa accctggtcc tatggcccgg aaggtggacc tgaccagctg cgatagagag
     9481 ccaatccaca tccccggctc catccagcca tgcggatgtc tgctggcatg tgacgcacag
     9541 gccgtgcgga tcaccagaat cacagagaac gcaggagcct tctttggaag ggagacccca
     9601 agggtgggag agctgctggc cgattacttc ggagagacag aggcacacgc actgaggaat
     9661 gcactggcac agagctccga ccccaagaga cctgccctga tcttcggatg gagggatgga
     9721 ctgaccggaa gaacatttga catctccctg caccggcacg atggcacatc tatcatcgag
     9781 tttgagccag cagcagcaga gcaggcagac aacccactga ggctgacccg ccagatcatc
     9841 gccagaacaa aggagctgaa gagcctggag gagatggcag caagggtgcc tagatacctg
     9901 caggccatgc tgggctacca cagagtgatg ctgtatagat tcgccgacga tggctctggc
     9961 atggtcatcg gcgaggccaa gaggagcgac ctggagtcct tcctgggaca gcactttcca
    10021 gcatccctgg tgcctcagca ggcaaggctg ctgtatctga agaacgccat ccgcgtggtg
    10081 tccgattcta ggggcatctc tagccgcatc gtgccagagc acgacgcatc tggagccgcc
    10141 ctggatctga gcttcgccca cctgaggagc atctcccctt gccacctgga gtttctgcgc
    10201 aatatgggcg tgtctgccag catgtccctg tctatcatca tcgacggcac cctgtggggc
    10261 ctgatcatct gtcaccacta tgagcctagg gcagtgccaa tggcacagag ggtggcagca
    10321 gagatgttcg ccgattttct gtccctgcac tttacagccg cccaccacca gcggtgagta
    10381 caagtaaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
    10441 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
    10501 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
    10561 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
    10621 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
    10681 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
    10741 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
    10801 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
    10861 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
    10921 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
    10981 cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac
    11041 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
    11101 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
    11161 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
    11221 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
    11281 tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
    11341 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
    11401 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
    11461 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
    11521 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
    11581 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
    11641 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
    11701 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
    11761 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
    11821 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
    11881 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
    11941 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
    12001 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
    12061 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
    12121 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    12181 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
    12241 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
    12301 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
    12361 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
    12421 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
    12481 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
    12541 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
    12601 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
    12661 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
    12721 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
    12781 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
    12841 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
    12901 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
    12961 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
    13021 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    13081 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
    13141 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    13201 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    13261 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    13321 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg ttt
//
