LOCUS       sequence-367470-       10600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NUP1 CEN URA3 plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10600)
  AUTHORS   Bogerd AM, Hoffman JA, Amberg DC, Fink GR, Davis LI
  TITLE     nup1 mutants exhibit pleiotropic defects in nuclear pore complex 
            function.
  JOURNAL   J Cell Biol. 1994 Oct . 127(2):319-32.
  PUBMED    7929578
REFERENCE   2  (bases 1 to 10600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 1994 Oct . 127(2):319-32."
COMMENT     Sequence Label: LDB59
FEATURES             Location/Qualifiers
     source          1..10600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        194..409
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             410..1213
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1344..1799)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1481..1502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1693..1712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1925..1947
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1939..1956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1940..1956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1963..1982
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1963..1981
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2014..2030
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2014..2030
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(7827..7845)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7866..7882)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7866..7882)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7879..7901)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7890..7906
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7914..7920,7921..7938,7939..7944))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7959..7980
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8097..8114)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8268..8856)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8348..8367)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9027..9818,9819..9887))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9650..9669
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9888..9992)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    10029..10532
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     10573..10591
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
      421 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
      481 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
      541 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
      601 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
      661 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
      721 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
      781 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
      841 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
      901 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
      961 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     1021 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     1081 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     1141 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     1201 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     1261 agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg cacagatgcg
     1321 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     1381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1681 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1741 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1801 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     1861 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     1921 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag
     1981 ggcgaattgg agctccaccg cggtggcggc cgctctagaa ctagtggatc caagcttatc
     2041 gatttcgaac ccggggtacc gaattcatca gtgaactctt catcattcaa aaacacccaa
     2101 tcatagttga acttggagtt aaatctatct tccacggatt taatagactc agccaacgaa
     2161 tatagatctc tattacgcac tagagtgaca aaagtggcct tttcccgagg accggtatac
     2221 tttggtagtt ccactttcga gtatttatag cccgagccgg aagatagcac ggtactgtac
     2281 tttgggcttg gggcagagcc tctcgcatac tgagctccat gaaagaacac atacacagta
     2341 aacacggcga ccagaagaag tcctaatttt ttgtaaacag gctgcttgct agctgggatc
     2401 ataatcttcg ccattttggt tattgactct atcccttaaa aactcttctg atggagtact
     2461 ttacttcgat tgcttaacga aatctttgtg aggaaaaaga tatctcttaa aattagaaag
     2521 tacaatagtc tagcgtatta tactaagaat ctgcaaaaaa gaagcaagaa ggcaccacct
     2581 attatagacc tttgacacga agtctctctg gagtgctttg gcctacgtgt gcggtactcg
     2641 tttacatggg acaaccacgg tttttttttt ggtgttactg gaggtataca gtgcgtatat
     2701 ccacttgtac gacaagagat ttacactaca ccgcgtaaag aaaacgccga caccaaatat
     2761 aagtcacgtg tatgcaaagc ctattttatg ccctaatttt caagccccgg tttttacgcc
     2821 ctagttttta tatttagggt ttgtcgttgc acgtgatcaa tggttcgtat tatgtgacat
     2881 tgaaatgctt tttcatttta atttttttct ttgacgaaat ttcgtaatgt caagaaacac
     2941 ttaaagaaaa taagtgatga ggaactcaat aaggacacta cgtagcggtg caaatacgat
     3001 aggatattag cctcgaaagg gttataggga cagagagtga gcgacaattt ttagtcattc
     3061 atgtcttcaa acacttcttc tgtgatgtct tctccacgtg tcgaaaagag atcgttttct
     3121 tccactttaa aatcattctt cacaaacccc aataaaaaac ggccatcgag caagaaagtt
     3181 ttcagttcaa acctctcata cgcgaatcat ttggaggaat cagatgttga agacacactg
     3241 catgttaata agagaaagag ggtgtccggt acatcacagc atagcgacag cttaactcag
     3301 aacaacaata atgcgccaat tataatatat ggaaccgaaa acactgagag accgccgctt
     3361 ttgccaattt tgcccattca aagactgagg ttattaaggg agaagcagag ggtgagaaat
     3421 atgcgtgagc ttggattaat tcaatcaact gaatttccat ctattacatc gtcggttata
     3481 ttgggctctc aaagtaaaag cgatgaggga ggatcgtacc tatgcacatc atctactcct
     3541 tcccctatta aaaacggttc ttgcactagg cagttggccg gaaaaagcgg tgaagacacc
     3601 aatgttggac tacccattct caaatcattg aaaaatagat ccaatagaaa aaggtttcat
     3661 agtcagtcaa aggggaccgt gtggtcagca aattttgaat atgatttgtc agaatatgac
     3721 gctatacaaa aaaaggataa caaggataag gaaggtaacg ctggcggtga tcagaagaca
     3781 agcgagaata gaaataatat taagagtagt atttcaaatg gcaatctggc tacaggccct
     3841 aacctgacaa gcgaaattga agacctacgt gcagacatca actctaatag gttatcgaat
     3901 cctcaaaaaa atctactttt aaaaggacca gcttccacag ttgcaaaaac tgcccctatt
     3961 caggagagct ttgttcccaa ttcagagcgc tctggtacgc ctacgttaaa gaaaaatatt
     4021 gagcccaaaa aggacaaaga aagtattgtt ttgcccaccg taggttttga ctttatcaag
     4081 gacaatgaga ctccatctaa gaaaacttct cctaaggcaa cttcttctgc aggtgcagtc
     4141 tttaaatcga gtgtagaaat gggaaaaacc gataagtcaa cgaaaactgc cgaggcgcct
     4201 accttatcat tcaattttag ccaaaaggct aataaaacta aggctgtcga caatactgtc
     4261 ccttccacaa ctttattcaa ttttggtggt aaatcagata ccgttacttc tgccagtcaa
     4321 ccttttaaat ttggaaagac atccgaaaaa agtgaaaatc atacagaatc agacgcgcct
     4381 ccaaaatcaa ctgctccaat attttctttt ggtaaacaag aagagaatgg tgatgaaggt
     4441 gatgatgaaa atgagcccaa aagaaaaagg cgtttacctg ttagcgagga tacaaacacc
     4501 aagcctttat tcgatttcgg caagaccggt gatcaaaagg agaccaaaaa gggagagtca
     4561 gaaaaggacg catcagggaa accaagcttt gtctttggtg caagtgataa gcaagctgaa
     4621 ggtacaccat tatttacatt cggaaaaaaa gctgatgtaa caagcaatat tgactcctct
     4681 gcacaattta cctttggtaa agccgccacc gcgaaagaaa cacacaccaa accatctgag
     4741 acacctgcca caatagtcaa gaagcctact tttacttttg ggcagtcaac aagtgaaaat
     4801 aagatctctg agggaagtgc gaaacctaca ttctctttct ctaagtcaga ggaggaacgt
     4861 aagagtagtc caatttcaaa cgaagcagct aaaccctcgt tttcgtttcc gggcaagcct
     4921 gttgatgttc aagcaccgac ggatgataag actctcaagc caactttttc ttttactgaa
     4981 cctgctcaaa aagattcatc tgttgtttcg gaacctaaaa agccctcctt tacgtttgcg
     5041 tcttcaaaaa cctcacaacc aaagccattg ttttcatttg gtaagtcaga tgcagctaaa
     5101 gaaccaccag gctctaacac ctcattttct ttcactaaac ctcctgctaa tgagacagat
     5161 aaaagaccta caccgccatc tttcaccttt ggcggttcca caacaaataa tacaacaacc
     5221 actagcacaa aaccatcttt tagttttggg gctcccgagt cgatgaagtc gacagcaagt
     5281 acagcggcag caaatacgga gaagctatca aatggctttt cctttacaaa gttcaatcac
     5341 aataaagaaa agtcaaactc tccaacttct ttcttcgatg gttctgcttc ctcaacgccg
     5401 attcctgtct tgggtaagcc aacagacgct actggtaata caacatctaa atctgcattt
     5461 tcattcggta ctgctaacac caatggtacc aatgcctcag caaactccac atcattctcg
     5521 tttaacgccc ctgctactgg taacggcaca actactactt ccaatacctc aggaaccaat
     5581 atagcgggta catttaacgt aggaaaaccg gatcaaagta tcgctagtgg caatacgaat
     5641 ggagcgggct cggcatttgg cttttcgagc tcaggaacag cagcaactgg tgcagcttct
     5701 aatcaatctt catttaattt tggaaacaat ggtgcagggg gtctcaatcc ttttacatca
     5761 gcaacttcgt caactaatgc taatgctggt ttattcaata aacctccttc cacgaatgca
     5821 caaaatgtca atgttccctc tgcttttaat tttacgggaa ataattcaac gcctggtggc
     5881 ggctctgtat ttaatatgaa cggcaacact aatgctaata cggtgtttgc cggctctaat
     5941 aaccaaccac atcaatcgca aaccccatct ttcaatacaa acagctcatt cacgccatca
     6001 acagttccta atattaattt tagcggattg aatggcggaa ttactaatac cgcgaccaat
     6061 gcattaaggc caagtgatat atttggtgcg aatgctgcct ctggttccaa ttcaaacgta
     6121 acaaatccat catccatttt tgggggggca ggtggtgtgc cgacaacttc ttttgggcag
     6181 ccgcagtcag cccctaatca gatggggatg ggaacaaata atggcatgag catgggcggt
     6241 ggtgttatgg cgaacagaaa gattgcaaga atgaggcact ctaaaaggta aatatcggtt
     6301 atgtaattag gtattgtgtt gcttttctga aggatatata ttcctatatt ccttcaactt
     6361 atacactgca tatgaaactt cttgagaagt tttaaataat tggtcttttt tttaatcggg
     6421 tacaggcgag ataatggata cctgttctag ggtaggcaaa tttggaggca agagcccaaa
     6481 attgttttgt tttgacctgg tttctctcag ttaacatatt atgaatagca tcttacaaat
     6541 aatttacgag cgaaactttc gatctgcaaa gcgctccttt tttctttccg aaaaaataca
     6601 gtaaaataag gtctagtaca cagcctgatc tacaattagt gttcttttct gtttacttcg
     6661 atcagctgtt tagctttgcc acttttctag tagctccaca cgctgtgaaa gtaacgagac
     6721 atagaagtca cggatggatt tgaaaaggga ttggttacga tggaaaatta caataggtag
     6781 tgggcctggc agtattgtgc tggatttccc atcatttttg gtagggtgcg tattcacaac
     6841 aatgatgggg ccaatattac agaagcttat aggtaaactt ttggtaggtt taataacggt
     6901 atgcaaattc ttagttatta ttggaagcat tgtcttcgtt atcggagttg catcaaagaa
     6961 atacacatat gacgatttca aagtaagtat aaaaagatca ggagagccag gggaatcaca
     7021 tgatatgagg actgaaccaa aaagaactgc gaaaacggca actgttccaa tggagaaaga
     7081 cgagggcgta ggttcttata attattttga aatacctata acgaaagaaa catctacaat
     7141 tccttatatt aattgtgatg gcacgagctc acttagaaag ccacccaacg gtccatcaag
     7201 cgtaggctta tctaactcca acagatacga aaattttata aacatggcac gacataaata
     7261 aatgtttgta tggcaacctg tcgatttagc ggcaatcata cggcggtgaa aaagtatgta
     7321 actatgcata gttgtcataa aatttatttg attcagatct ttgcaaaaag tatttttttt
     7381 ggaatttgga gtttttatta tcttattgtg ctataaaata aacaaagttg ttactaacta
     7441 ataataaaga tttagatatt attatattaa ctaatgagtt tcacttggaa tttcgaaaac
     7501 aatttgctta ccagtcaatt tgttgtaaac agcttggaaa gattccaatt tgtagtcgat
     7561 ttgttggaca tcctttgagt ctaacaaaac cttttggatc ttgttaccac caaccaaata
     7621 tctaactctc ttaccaacga tttcagttgg gaagactaag tcttccaaga tcttgtcatg
     7681 aacagcagtc aaggttctgg atcttggtct cttttggact tgtctagatg ttctagatgg
     7741 ctttggcaag attcttcttt cagccaagaa gatgacatga cggtcttgga attcctcgag
     7801 ggggggcccg gtaccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa
     7861 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7921 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7981 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     8041 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8101 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     8161 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8221 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8281 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8341 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8401 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8461 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8521 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8581 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8641 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8701 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8761 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8821 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8881 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8941 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9001 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9061 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9121 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9181 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9241 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9301 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9361 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9421 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9481 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9541 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9601 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9661 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9721 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9781 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9841 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9901 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9961 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg
    10021 tccttttcat cacgtgctat aaaaataatt ataatttaaa ttttttaata taaatatata
    10081 aattaaaaat agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga
    10141 tgtaaaagac tctaggggga tcgccaacaa atactacctt ttatcttgct cttcctgctc
    10201 tcaggtatta atgccgaatt gtttcatctt gtctgtgtag aagaccacac acgaaaatcc
    10261 tgtgatttta cattttactt atcgttaatc gaatgtatat ctatttaatc tgcttttctt
    10321 gtctaataaa tatatatgta aagtacgctt tttgttgaaa ttttttaaac ctttgtttat
    10381 ttttttttct tcattccgta actcttctac cttctttatt tactttctaa aatccaaata
    10441 caaaacataa aaataaataa acacagagta aattcccaaa ttattccatc attaaaagat
    10501 acgaggcgcg tgtaagttac aggcaagcga tccgtctaag aaaccattat tatcatgaca
    10561 ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
