LOCUS       sequence-367484-        9943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression of Nup1 N-terminal truncation (nup1-15 allele).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9943)
  AUTHORS   Bogerd AM, Hoffman JA, Amberg DC, Fink GR, Davis LI
  TITLE     nup1 mutants exhibit pleiotropic defects in nuclear pore complex 
            function.
  JOURNAL   J Cell Biol. 1994 Oct . 127(2):319-32.
  PUBMED    7929578
REFERENCE   2  (bases 1 to 9943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 1994 Oct . 127(2):319-32."
COMMENT     Sequence Label: LDB33
FEATURES             Location/Qualifiers
     source          1..9943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        191..471
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             472..1146
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1249..1704)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1386..1407)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1598..1617
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1830..1852
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1844..1861
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1845..1861
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1868..1887
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1868..1886
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1919..1935
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1919..1935
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(7171..7189)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7210..7226)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7210..7226)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7223..7245)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7234..7250
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7258..7264,7265..7282,7283..7288))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7303..7324
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7441..7458)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7612..8200)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7692..7711)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8371..9162,9163..9231))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8994..9013
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9232..9336)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    9373..9875
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9916..9934
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatagatc acgacattac tatatatata atataggaag catttaatag aacagcatcg
      241 taatatatgt gtactttgca gttatgacgc cagatggcag tagtggaaga tattctttat
      301 tgaaaaatag cttgtcacct tacgtacaat cttgatccgg agcttttctt tttttgccga
      361 ttaagaatta attcggtcga aaaaagaaaa ggagagggcc aagagggagg gcattggtga
      421 ctattgagca cgtgagtata cgtgattaag cacacaaagg cagcttggag tatgtctgtt
      481 attaatttca caggtagttc tggtccattg gtgaaagttt gcggcttgca gagcacagag
      541 gccgcagaat gtgctctaga ttccgatgct gacttgctgg gtattatatg tgtgcccaat
      601 agaaagagaa caattgaccc ggttattgca aggaaaattt caagtcttgt aaaagcatat
      661 aaaaatagtt caggcactcc gaaatacttg gttggcgtgt ttcgtaatca acctaaggag
      721 gatgttttgg ctctggtcaa tgattacggc attgatatcg tccaactgca tggagatgag
      781 tcgtggcaag aataccaaga gttcctcggt ttgccagtta ttaaaagact cgtatttcca
      841 aaagactgca acatactact cagtgcagct tcacagaaac ctcattcgtt tattcccttg
      901 tttgattcag aagcaggtgg gacaggtgaa cttttggatt ggaactcgat ttctgactgg
      961 gttggaaggc aagagagccc cgaaagctta cattttatgt tagctggtgg actgacgcca
     1021 gaaaatgttg gtgatgcgct tagattaaat ggcgttattg gtgttgatgt aagcggaggt
     1081 gtggagacaa atggtgtaaa agactctaac aaaatagcaa atttcgtcaa aaatgctaag
     1141 aaataggtta ttactgagta gtatttattt aagtattgtt tgtgcacttg ccgatctatg
     1201 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg
     1261 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     1321 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     1381 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     1441 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     1501 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     1561 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     1621 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     1681 ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg
     1741 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     1801 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     1861 tgaattgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc
     1921 tagaactagt ggatccaagc ttatcgattt cgaacccggg gtaccgaatt catcagtgaa
     1981 ctcttcatca ttcaaaaaca cccaatcata gttgaacttg gagttaaatc tatcttccac
     2041 ggatttaata gactcagcca acgaatatag atctctatta cgcactagag tgacaaaagt
     2101 ggccttttcc cgaggaccgg tatactttgg tagttccact ttcgagtatt tatagcccga
     2161 gccggaagat agcacggtac tgtactttgg gcttggggca gagcctctcg catactgagc
     2221 tccatgaaag aacacataca cagtaaacac ggcgaccaga agaagtccta attttttgta
     2281 aacaggctgc ttgctagctg ggatcataat cttcgccatt ttggttattg actctatccc
     2341 ttaaaaactc ttctgatgga gtactttact tcgattgctt aacgaaatct ttgtgaggaa
     2401 aaagatatct cttaaaatta gaaagtacaa tagtctagcg tattatacta agaatctgca
     2461 aaaaagaagc aagaaggcac cacctattat agacctttga cacgaagtct ctctggagtg
     2521 ctttggccta cgtgtgcggt actcgtttac atgggacaac cacggttttt tttttggtgt
     2581 tactggaggt atacagtgcg tatatccact tgtacgacaa gagatttaca ctacaccgcg
     2641 taaagaaaac gccgacacca aatataagtc acgtgtatgc aaagcctatt ttatgcccta
     2701 attttcaagc cccggttttt acgccctagt ttttatattt agggtttgtc gttgcacgtg
     2761 atcaatggtt cgtattatgt gacattgaaa tgctttttca ttttaatttt tttctttgac
     2821 gaaatttcgt aatgtcaaga aacacttaaa gaaaataagt gatgaggaac tcaataagga
     2881 cactacgtag cggtgcaaat acgataggat attagcctcg aaagggttat agggacagag
     2941 agtgagcgac aatttttagt cattcatgtc ttcagaaaat agatccaata gaaaaaggtt
     3001 tcatagtcag tcaaagggga ccgtgtggtc agcaaatttt gaatatgatt tgtcagaata
     3061 tgacgctata caaaaaaagg ataacaagga taaggaaggt aacgctggcg gtgatcagaa
     3121 gacaagcgag aatagaaata atattaagag tagtatttca aatggcaatc tggctacagg
     3181 ccctaacctg acaagcgaaa ttgaagacct acgtgcagac atcaactcta ataggttatc
     3241 gaatcctcaa aaaaatctac ttttaaaagg accagcttcc acagttgcaa aaactgcccc
     3301 tattcaggag agctttgttc ccaattcaga gcgctctggt acgcctacgt taaagaaaaa
     3361 tattgagccc aaaaaggaca aagaaagtat tgttttgccc accgtaggtt ttgactttat
     3421 caaggacaat gagactccat ctaagaaaac ttctcctaag gcaacttctt ctgcaggtgc
     3481 agtctttaaa tcgagtgtag aaatgggaaa aaccgataag tcaacgaaaa ctgccgaggc
     3541 gcctacctta tcattcaatt ttagccaaaa ggctaataaa actaaggctg tcgacaatac
     3601 tgtcccttcc acaactttat tcaattttgg tggtaaatca gataccgtta cttctgccag
     3661 tcaacctttt aaatttggaa agacatccga aaaaagtgaa aatcatacag aatcagacgc
     3721 gcctccaaaa tcaactgctc caatattttc ttttggtaaa caagaagaga atggtgatga
     3781 aggtgatgat gaaaatgagc ccaaaagaaa aaggcgttta cctgttagcg aggatacaaa
     3841 caccaagcct ttattcgatt tcggcaagac cggtgatcaa aaggagacca aaaagggaga
     3901 gtcagaaaag gacgcatcag ggaaaccaag ctttgtcttt ggtgcaagtg ataagcaagc
     3961 tgaaggtaca ccattattta cattcggaaa aaaagctgat gtaacaagca atattgactc
     4021 ctctgcacaa tttacctttg gtaaagccgc caccgcgaaa gaaacacaca ccaaaccatc
     4081 tgagacacct gccacaatag tcaagaagcc tacttttact tttgggcagt caacaagtga
     4141 aaataagatc tctgagggaa gtgcgaaacc tacattctct ttctctaagt cagaggagga
     4201 acgtaagagt agtccaattt caaacgaagc agctaaaccc tcgttttcgt ttccgggcaa
     4261 gcctgttgat gttcaagcac cgacggatga taagactctc aagccaactt tttcttttac
     4321 tgaacctgct caaaaagatt catctgttgt ttcggaacct aaaaagccct cctttacgtt
     4381 tgcgtcttca aaaacctcac aaccaaagcc attgttttca tttggtaagt cagatgcagc
     4441 taaagaacca ccaggctcta acacctcatt ttctttcact aaacctcctg ctaatgagac
     4501 agataaaaga cctacaccgc catctttcac ctttggcggt tccacaacaa ataatacaac
     4561 aaccactagc acaaaaccat cttttagttt tggggctccc gagtcgatga agtcgacagc
     4621 aagtacagcg gcagcaaata cggagaagct atcaaatggc ttttccttta caaagttcaa
     4681 tcacaataaa gaaaagtcaa actctccaac ttctttcttc gatggttctg cttcctcaac
     4741 gccgattcct gtcttgggta agccaacaga cgctactggt aatacaacat ctaaatctgc
     4801 attttcattc ggtactgcta acaccaatgg taccaatgcc tcagcaaact ccacatcatt
     4861 ctcgtttaac gcccctgcta ctggtaacgg cacaactact acttccaata cctcaggaac
     4921 caatatagcg ggtacattta acgtaggaaa accggatcaa agtatcgcta gtggcaatac
     4981 gaatggagcg ggctcggcat ttggcttttc gagctcagga acagcagcaa ctggtgcagc
     5041 ttctaatcaa tcttcattta attttggaaa caatggtgca gggggtctca atccttttac
     5101 atcagcaact tcgtcaacta atgctaatgc tggtttattc aataaacctc cttccacgaa
     5161 tgcacaaaat gtcaatgttc cctctgcttt taattttacg ggaaataatt caacgcctgg
     5221 tggcggctct gtatttaata tgaacggcaa cactaatgct aatacggtgt ttgccggctc
     5281 taataaccaa ccacatcaat cgcaaacccc atctttcaat acaaacagct cattcacgcc
     5341 atcaacagtt cctaatatta attttagcgg attgaatggc ggaattacta ataccgcgac
     5401 caatgcatta aggccaagtg atatatttgg tgcgaatgct gcctctggtt ccaattcaaa
     5461 cgtaacaaat ccatcatcca tttttggggg ggcaggtggt gtgccgacaa cttcttttgg
     5521 gcagccgcag tcagccccta atcagatggg gatgggaaca aataatggca tgagcatggg
     5581 cggtggtgtt atggcgaaca gaaagattgc aagaatgagg cactctaaaa ggtaaatatc
     5641 ggttatgtaa ttaggtattg tgttgctttt ctgaaggata tatattccta tattccttca
     5701 acttatacac tgcatatgaa acttcttgag aagttttaaa taattggtct tttttttaat
     5761 cgggtacagg cgagataatg gatacctgtt ctagggtagg caaatttgga ggcaagagcc
     5821 caaaattgtt ttgttttgac ctggtttctc tcagttaaca tattatgaat agcatcttac
     5881 aaataattta cgagcgaaac tttcgatctg caaagcgctc cttttttctt tccgaaaaaa
     5941 tacagtaaaa taaggtctag tacacagcct gatctacaat tagtgttctt ttctgtttac
     6001 ttcgatcagc tgtttagctt tgccactttt ctagtagctc cacacgctgt gaaagtaacg
     6061 agacatagaa gtcacggatg gatttgaaaa gggattggtt acgatggaaa attacaatag
     6121 gtagtgggcc tggcagtatt gtgctggatt tcccatcatt tttggtaggg tgcgtattca
     6181 caacaatgat ggggccaata ttacagaagc ttataggtaa acttttggta ggtttaataa
     6241 cggtatgcaa attcttagtt attattggaa gcattgtctt cgttatcgga gttgcatcaa
     6301 agaaatacac atatgacgat ttcaaagtaa gtataaaaag atcaggagag ccaggggaat
     6361 cacatgatat gaggactgaa ccaaaaagaa ctgcgaaaac ggcaactgtt ccaatggaga
     6421 aagacgaggg cgtaggttct tataattatt ttgaaatacc tataacgaaa gaaacatcta
     6481 caattcctta tattaattgt gatggcacga gctcacttag aaagccaccc aacggtccat
     6541 caagcgtagg cttatctaac tccaacagat acgaaaattt tataaacatg gcacgacata
     6601 aataaatgtt tgtatggcaa cctgtcgatt tagcggcaat catacggcgg tgaaaaagta
     6661 tgtaactatg catagttgtc ataaaattta tttgattcag atctttgcaa aaagtatttt
     6721 ttttggaatt tggagttttt attatcttat tgtgctataa aataaacaaa gttgttacta
     6781 actaataata aagatttaga tattattata ttaactaatg agtttcactt ggaatttcga
     6841 aaacaatttg cttaccagtc aatttgttgt aaacagcttg gaaagattcc aatttgtagt
     6901 cgatttgttg gacatccttt gagtctaaca aaaccttttg gatcttgtta ccaccaacca
     6961 aatatctaac tctcttacca acgatttcag ttgggaagac taagtcttcc aagatcttgt
     7021 catgaacagc agtcaaggtt ctggatcttg gtctcttttg gacttgtcta gatgttctag
     7081 atggctttgg caagattctt ctttcagcca agaagatgac atgacggtct tggaattcct
     7141 cgaggggggg cccggtacca gcttttgttc cctttagtga gggttaattt cgagcttggc
     7201 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7261 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7321 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7381 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7441 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7501 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7561 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7621 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7681 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7741 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7801 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7861 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7921 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7981 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8041 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8101 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8161 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8221 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8281 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8341 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8401 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8461 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8521 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8581 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8641 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8701 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8761 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8821 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8881 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8941 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9001 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9061 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9121 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9181 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9241 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9301 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9361 tgggtccttt tcatcacgtg ctataaaaat aattataatt taaatttttt aatataaata
     9421 tataaattaa aaatagaaag taaaaaaaga aattaaagaa aaaatagttt ttgttttccg
     9481 aagatgtaaa agactctagg gggatcgcca acaaatacta ccttttatct tgctcttcct
     9541 gctctcaggt attaatgccg aattgtttca tcttgtctgt gtagaagacc acacacgaaa
     9601 atcctgtgat tttacatttt acttatcgtt aatcgaatgt atatctattt aatctctttt
     9661 cttgtctaat aaatatatat gtaaagtacg ctttttgttg aaatttttta aacctttgtt
     9721 tatttttttt tcttcattcc gtaactcttc taccttcttt atttactttc taaaatccaa
     9781 atacaaaaca taaaaataaa taaacacaga gtaaattccc aaattattcc atcattaaaa
     9841 gatacgaggc gcgtgtaagt tacaggcaag cgatccgtct aagaaaccat tattatcatg
     9901 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
