LOCUS       sequence-367516-       11651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Regulated expression of the DddA immunity determinant (DddAI) in 
            Firmicutes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11651)
  AUTHORS   Gallagher LA, Velazquez E, Peterson SB, Charity JC, Radey MC, 
            Gebhardt MJ, Hsu F, Shull LM, Cutler KJ, Macareno K, de Moraes MH, 
            Penewit KM, Kim J, Andrade PA, LaFramboise T, Salipante SJ, Reniere 
            ML, de Lorenzo V, Wiggins PA, Dove SL, Mougous JD
  TITLE     Genome-wide protein-DNA interaction site mapping in bacteria using a
            double-stranded DNA-specific cytosine deaminase.
  JOURNAL   Nat Microbiol. 2022 Jun;7(6):844-855. doi: 
            10.1038/s41564-022-01133-9. Epub 2022 Jun 1.
  PUBMED    35650286
REFERENCE   2  (bases 1 to 11651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-022-01133-9"; journalName: "Nat Microbiol"; date: 
            "2022-06"; volume: "7"; issue: "6"; pages: "844-855"
COMMENT     Sequence Label: pBS10-riboE-dddAI
FEATURES             Location/Qualifiers
     source          1..11651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             457..465
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(900..918)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      914..961
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     1161..1180
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1580..1585,1586..1602,1603..1608))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      1720..2265
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2166..2185
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            2533..2642
                     /note="incP origin of transfer"
     primer_bind     2853..2870
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        3262..3364
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3365..4024
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3431..3450)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8918..9712
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEEYRCVKDTEGMSPAKVYKLVGENEILYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEGYED
                     EQSPEKTIELYAECIRLFHSIDISDCPYTNSLDGRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag
       61 ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc
      121 cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg
      181 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
      241 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc
      301 gaacgccagc aagacgtagc ccagcgcgtc ggccgaattt tgcaaaaagt tgttgacttt
      361 atctacaagg tgtggcataa tgtgtgtctc gagatacgac tcactatagg taccggtgat
      421 accagcatcg tcttgatgcc cttggcagca ccctgctaag gaggtaacaa caagatgatg
      481 tacgcagacg atttcgacgg cgaaattgag atcgatgagg ttgatagctt ggtcgagttc
      541 ctcagcagaa gacctgcatt tgacgcaaat aattttgttt tgacgtttga ggaaagtggt
      601 tttcctcaat tgaatatttt tgcaaaaaac gatatcgccg ttgtttatta tatggacatc
      661 ggcgagaatt tcgtttcgaa aggcaattct gcgtcaggtg gtacggagaa gttttacgag
      721 aataagcttg gcggggaagt agacctgtcg aaagattgcg ttgtgagtaa agagcaaatg
      781 attgaagcgg caaagcagtt ttttgctacg aagcaacggc ccgagcaact gacatggagc
      841 gagttgtgag agctcggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      901 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      961 ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     1021 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     1081 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     1141 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     1201 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tccacaggac gggtgtggtc
     1261 gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg
     1321 ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat
     1381 agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag
     1441 aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg
     1501 aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt
     1561 aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaatta
     1621 caacttatat cgtatggggc tgacttcagg tgctacattt gaagagataa attgcactga
     1681 aatctagaaa tattttatct gattaataag atgatcttct tgagatcgtt ttggtctgcg
     1741 cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt cgaaggttct
     1801 ctgagctacc aactctttga accgaggtaa ctggcttgga ggagcgcagt caccaaaact
     1861 tgtcctttca gtttagcctt aaccggcgca tgacttcaag actaactcct ctaaatcaat
     1921 taccagtggc tgctgccagt ggtgcttttg catgtctttc cgggttggac tcaagacgat
     1981 agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct
     2041 tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat
     2101 aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg
     2161 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag
     2221 cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg
     2281 gatgtatgct atacgaagtt atgcgatcgc ctctcgcctg tcccctcagt tcagtaattt
     2341 cctgcatttg cctgtttcca gtcggtagat attccacaaa acagcaggga agcagcgctt
     2401 ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt
     2461 tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca
     2521 acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt
     2581 cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc
     2641 ggctaccgcc ggcgtaacag atgagggcaa gcggcggaga attacaactt atatcgtatg
     2701 gggctgactt caggtgctac atttgaagag ataaattgca ctgaaaccgc ggccctctca
     2761 cttccctgtt aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca
     2821 tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat
     2881 atatcctgta tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg
     2941 aagcacttca ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag
     3001 cgctgatgtc cggcggtgct tttgccgtta cgcaccaccc cgtcagtagc tgaacaggag
     3061 ggacagctga tagaaacaga agccactgga gcacctcaaa aacaccatca tacactaaat
     3121 cagtaagttg gcagcatcac ccgacgcact ttgcgccgaa taaatacctg tgacggaaga
     3181 tcacttcgca gaataaataa atcctggtgt ccctgttgat accgggaagc cctgggccaa
     3241 cttttggcga aaatgagacg ttgatcggca cgtaagaggt tccaactttc accataatga
     3301 aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc
     3361 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     3421 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     3481 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     3541 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     3601 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     3661 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     3721 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     3781 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     3841 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     3901 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     3961 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     4021 gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggttgctacg cctgaataag
     4081 tgataataag cggatgaatg gcagaaattc gaaagcaaat tcgacccggt cgtcggttca
     4141 gggcagggtc gttaaatagc cgcttatgtc tattgctggt ttaccggttt attgactacc
     4201 ggaagcagtg tgaccgtgtg cttctcaaat gcctgaggcc agtttgctca ggctctcccc
     4261 gtggaggtaa taattgacga tatgatcatt tattctgcct cccagagcct gataaaaacg
     4321 gttagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga
     4381 tgcagatccg gaacataatg gtgcagggcg cttgtttcgg cgtgggtatg gtggcaggcc
     4441 ccgtggccgg gggactgttg ggcgctgccg gcacctgtcc tacgagttgc atgataaaga
     4501 agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctaccgg
     4561 cagcggtgcg gactgttgta actcagaata agaaatgagg ccgctcatgg cgttgactct
     4621 cagtcatagt atcgtggtat caccggttgg ttccactctc tgttgcgggc aacttcagca
     4681 gcacgtaggg gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca
     4741 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat
     4801 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga
     4861 caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgact ttaaacgtgg
     4921 atcattttct ttaaatttat gctgacgacc tttgaatttg ccttttttct tagcaatttc
     4981 gattccttgt gcctgacgtt ccttaatttt ttttcgttct gattctgctt gatacttgta
     5041 caattcaatg acaaggctat taatcaaacg ccttaaattt tcatcttcaa taccattcat
     5101 tgagggtaaa tttaagactt ccagggttgc ccccttaatt tgaatttgat tcatcaattc
     5161 tgttaattct ttattatttc gtcctaatcg atctaattca gtaacaataa caatatcccc
     5221 ttcacgaata tagttaagca tagcttgtaa ttgtgggcgt tcgaccgatt gaccgcttaa
     5281 tttgtctgaa aagaccttag aaacgccctg taacgcttgt aattgccgat ctaagttctg
     5341 ttctttgcta ctgacacgtg cataaccaat tttagccatt ttcaaccaac ctctaaaatt
     5401 ctctcggttg caataaccaa tcagcaatat ctactttttc aatttcaaat tgcttatcag
     5461 aaattgtctt ttcgtaagcg ataaaatctt gcgcatattg ttgctcatta aaaatagcca
     5521 ccacttcgtc attttctaaa actcgataaa taaatttttt cattttactc ctcctattat
     5581 gcccaactta aatgacctat tcaccaagtc aattatactg ctaaaatcat attaggacaa
     5641 ataggtatac tctattgacc tataaatgat agcaacttaa aagatcaagt gttcgcttcg
     5701 ctctcactgc ccctcgacgt tttagtagcc tttccctcac ttcgttcagt ccaagccaac
     5761 taaaagtttt cgggctactc tctccttctc cccctaataa ttaattaaaa tcttactctg
     5821 tatatttctg ctaatcattc actaaacagc aaagaaaaac aaacacgtat catagatata
     5881 aatgtaatgg catagtgcgg gttttatttt cagcctgtat cgtagctaaa caaatcgagt
     5941 tgtgggtccg ttttggggcg ttctgccaat ttgtttagag tttcttgaat aaatgtacgt
     6001 tctaaattaa acgaagctgt cagcgccttt atatagcttt ctcgttcttc tttttttaat
     6061 ttaatgatcg atagcaacaa tgatttaaca ctagcaagtt gaatgccacc atttcttcct
     6121 ggtttaatct taaagaaaat ttcctgattc gccttcagta ccttcagcaa tttatctaat
     6181 gtccgttcag gaatgcctag cacttctcta atctcttttt tggtcgtcgc taaataaggc
     6241 ttgtatacat cgcttttttc gctaatataa gccattaaat cttctttcca ttctgacaaa
     6301 tgaacacgtt gacgttcgct tctttttttc ttgaatttaa accacccttg acggacaaat
     6361 aaatctttac tggttaaatc acttgatacc caagctttgc aaagaatggt aatgtattcc
     6421 ctattagccc cttgatagtt ttctgaatag gcacttctaa caattttgat tacttctttt
     6481 tcttctaagg gttgatctaa tcgattatta aactcaaaca tattatattc gcacgtttcg
     6541 attgaatagc ctgaactaaa gtaggctaaa gagagggtaa acataacgct attgcgccct
     6601 actaaaccct tttctcctga aaatttcgtt tcgtgcaata agagattaaa ccagggttca
     6661 tctacttgtt ttttgccttc tgtaccgctt aaaaccgtta gacttgaacg agtaaagccc
     6721 ttattatctg tttgtttgaa agaccaatct tgccattctt tgaaagaata acggtaattg
     6781 ggatcaaaaa attctacatt gtccgttctt ggtatacgag caatcccaaa atgattgcac
     6841 gttagatcaa ctggcaaaga ctttccaaaa tattctcgga tattttgcga gattattttg
     6901 gctgctttga cagatttaaa ttctgatttt gaagtcacat agactggcgt ttctaaaaca
     6961 aaatatgctt gataaccttt atcagatttg ataattaacg taggcataaa acctaaatca
     7021 atagctgttg ttaaaatatc gcttgctgaa atagtttctt tttccgtgtg aatatcaaaa
     7081 tcaataaaga aggtattgat ttgtcttaaa ttgttttcag aatgtccttt agtgtatgaa
     7141 cggttttcgt ctgcatacgt accataacga taaacgtttg gtgtccaatg cgtaaatgta
     7201 tcttgatttt cgtgaatcgc ttcttcggaa gtcagaacaa cgccacgtcc gccaatcatg
     7261 cttttttttg agcgatacgc aaaaatagcc cctttacttt tacctggctt ggtagtgatt
     7321 gagcgaattt tactattttt aaatttgtac tttaacaagc cgtcatgaag cacagtttct
     7381 acaacaaaag ggatattcat tcagctgttc tcctttctta cgaaaattaa ttagttagaa
     7441 gctacgatca aagttgaatc acaacaaaaa aggcaatcaa ctaagttttt cttaattgat
     7501 tgcctggtat cttcttaaag acttgaaatc ccctcaaaaa cccgatataa tgggtttaca
     7561 gatatttaag tatctgatta ataaagtaat taaatacttt accaaatttt gggtctcgac
     7621 ttctttaatt gattggtggt aatcaattaa ggctcgcagt taaagtttct caggctttaa
     7681 ctggtcgtgg ctcttttttt gtattcttta ttcagttcgt tgtttcgtta tatctagtat
     7741 atcgcttttt aaaaaaataa gcaatgattt cgtgcattat tcacacgaaa tcattgcttt
     7801 tttcttcttc catttctaac tccaatgtta cttgttctgt ttctggttct ggttctgttg
     7861 gctcatttgg gattaaatcc actactagcg ttgagttagt tccgtctcta atagccggtt
     7921 aagtaatagc cggttaagtg gtcaaacttt gggaaaatct caacccgcat taagttttga
     7981 tgccatgaca atcgttggaa atttgaacaa aactaatgct aaaaagctat ctgactttat
     8041 gagtgtagag ccacaaatac gactttggga tatacttcaa acaaagttta aagctaaggc
     8101 acttcaagaa aaagtttata tcgaatatga caaagtaaaa gcagatactt gggatagacg
     8161 taatatgcgt gttgaattta atcccaataa actcacacat gaagaaatga tttggttaaa
     8221 acaaaatatt atcgactaca tggaagatga cggttttaca agattagact tagcttttga
     8281 ttttgaagat gatttgagcg attactatgc aatgactgat aaagcagtta agaaaactgt
     8341 tttttatggt cgtaatggca agccagaaac aaaatatttt ggtgtccgtg atagtgatag
     8401 atttattaga atttataata aaaaacaaga acgtaaagat aacgcagatg ttgaagttgt
     8461 gtctgaacat ttatggcgtg tagaagttga attaaaaaga gatatggttg attactggaa
     8521 tgattgtttt aatgatttac acatcttgaa acctgcgtgg gctactttag aaaaaattaa
     8581 tgagcaagct atggtttata ctttgttgca tgaagaaagt atgtggggaa agctaagtaa
     8641 gaatactaag actaaattta aaaaattgat tagagaaata tctccaattg atttaacgga
     8701 attaatgaaa tcgactttaa aagcgaacga aaaacaattg caaaagcaga ttgatttttg
     8761 gcaacgtgaa tttaggtttt ggaagtaaaa taagttttat ttgataaaaa ttgctaattc
     8821 agtataatta atatttacga ggtgacataa cgtatgaaaa aatcagagga ttattcctcc
     8881 taaatataaa aatttaaaat ttaggaggaa gttatatatg gctaaaatga gaatatcacc
     8941 ggaattgaaa aaactgatcg aagaataccg ctgcgtaaaa gatacggaag gaatgtctcc
     9001 tgctaaggta tataagctgg tgggagaaaa tgaaatccta tatttaaaaa tgacggacag
     9061 ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga
     9121 aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct
     9181 gctcatgagt gaggccgatg gcgtcctttg ctcggaaggg tatgaagatg aacaaagccc
     9241 tgaaaagact atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc
     9301 ggattgtccc tatacgaata gcttagacgg ccgcttagcc gaattggatt acttactgaa
     9361 taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc
     9421 gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca
     9481 cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga
     9541 tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat
     9601 cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa
     9661 gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt agtaccgagt
     9721 agtttttaga ttttgaaagt gaatttaatt ttatacacgt aagtgatcat aaaatttatg
     9781 aacgtataac aaccacattt tttggttgct tgtggttttg attttgaatt tggttttgaa
     9841 cttatggact gatttattca gtccattttt tgtgcttgca caaaaactag cctcgcagag
     9901 cacacgcatt aatgacttat gaaacgtagt aaataagtct agtgtgttat actttacttg
     9961 gaagatgcac cgaataaaaa atattgaaga acaactagca aaagatttta aagagttatt
    10021 ttattttaag tctttataac atgagtgaag cgaattttta aatttcgata gaaattttta
    10081 catcaaaaag ccccctgtca aaattgacga agggggtttt ttggcgcacg cttttcgtta
    10141 gaaatataca agattgaaaa tcgtgtataa gtgcgccctt tgttttgaac ttagcacgtt
    10201 acatcaattt tttaaaatga tgtataagtg cgccctttta aattttgagt gattatattt
    10261 tttgagttag aaaaagggat tgggaaaatt tcccaaaata atttaaaaaa taagcaaaaa
    10321 ttttcgatag agaatgtgct attttttgtc aaaggtgtat accttgactg tgcttgctgt
    10381 tacattaagt ttatttttaa gttattaaaa aagaaatagc ttttaaagtt tggctcgctg
    10441 tcgctttata aagctgattg acttttgatt gcaaactact taaagaaaac aaactcggac
    10501 tattcgtttt cttctctttg gtttgaacat cagcaattat cccctcttga ttgcctattt
    10561 tagcttgttt agaagaaaca aaagctaaaa gctcctcttg ggttttaaaa cgctgtgtgg
    10621 ggcttagaac gcccttaaac gacccttggt ttacttttat actagcttcc acctcgaaaa
    10681 aaggttcttt tttaaaattc tctatggctt cctggcgctg aaaaataagg tataaggtgg
    10741 gcgtttgaac acgtcctagt gaaaatgtac cttgtacgcc cttctgttgt aaatttaacg
    10801 tatacaaagg gcttgcgttc atgccgatca accaatcggc aatttggcgt gtttgcgctt
    10861 cttgataaaa gggatagtaa ttcattccag gttgcaaatt ttgaaaaccg cttcggatta
    10921 catctttttc taagctattg atccatagtc ttttaaatgt tttatctttt gaaaaggcat
    10981 ttgctttatg gataatcgac caggcgatat tttcaccttc tctgtcgcta tctgttgcaa
    11041 caataattgt atttgccttt ttgagaagtt ctgcaacaat tttaaactgc tttcccttat
    11101 cttttgcaac ttcaaaatcg tatcgatcag gaaaaatcgg caaagattca agtttccaat
    11161 tttgccactt ttcgtcataa tgacctggtt ctgctaattc cactaaatgc ccaaaaccaa
    11221 aggtgataaa cgtttcatct gtaaatagtg ggtctttgat ctcaaaataa ccgtcttttt
    11281 tggtgctttg ttttaaagca cttgcgtagg ctaatgcctg gcttggtttt tcagctaaaa
    11341 taaccgtact cattaactat ccctcttttc attgtttttt ctttgatcga ctgtcacgtt
    11401 atatcttgct cgataccttc taaacgttcg gcgattgatt ccagtttgtt cttcaacttc
    11461 tttatcggat aaaccattca aaaacaaatc gaaagccgag atgcgccgcg tgcggctgct
    11521 ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct
    11581 ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg
    11641 gcttccattc a
//
