LOCUS       sequence-367520-       11675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Regulated expression in Firmicutes of the DddA immunity determinant 
            with a C-terminal fusion to a FLAG epitope (DddAI-FLAG).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11675)
  AUTHORS   Gallagher LA, Velazquez E, Peterson SB, Charity JC, Radey MC, 
            Gebhardt MJ, Hsu F, Shull LM, Cutler KJ, Macareno K, de Moraes MH, 
            Penewit KM, Kim J, Andrade PA, LaFramboise T, Salipante SJ, Reniere 
            ML, de Lorenzo V, Wiggins PA, Dove SL, Mougous JD
  TITLE     Genome-wide protein-DNA interaction site mapping in bacteria using a
            double-stranded DNA-specific cytosine deaminase.
  JOURNAL   Nat Microbiol. 2022 Jun;7(6):844-855. doi: 
            10.1038/s41564-022-01133-9. Epub 2022 Jun 1.
  PUBMED    35650286
REFERENCE   2  (bases 1 to 11675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-022-01133-9"; journalName: "Nat Microbiol"; date: 
            "2022-06"; volume: "7"; issue: "6"; pages: "844-855"
COMMENT     Sequence Label: pBS10-ribE-dddAI-FLAG
FEATURES             Location/Qualifiers
     source          1..11675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             457..465
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             847..870
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(924..942)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      938..985
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     1185..1204
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1604..1609,1610..1626,1627..1632))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      1744..2289
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2190..2209
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            2557..2666
                     /note="incP origin of transfer"
     primer_bind     2877..2894
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        3286..3388
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3389..4048
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3455..3474)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8942..9736
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEEYRCVKDTEGMSPAKVYKLVGENEILYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEGYED
                     EQSPEKTIELYAECIRLFHSIDISDCPYTNSLDGRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag
       61 ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc
      121 cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg
      181 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
      241 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc
      301 gaacgccagc aagacgtagc ccagcgcgtc ggccgaattt tgcaaaaagt tgttgacttt
      361 atctacaagg tgtggcataa tgtgtgtctc gagatacgac tcactatagg taccggtgat
      421 accagcatcg tcttgatgcc cttggcagca ccctgctaag gaggtaacaa caagatgatg
      481 tacgcagacg atttcgacgg cgaaattgag atcgatgagg ttgatagctt ggtcgagttc
      541 ctcagcagaa gacctgcatt tgacgcaaat aattttgttt tgacgtttga ggaaagtggt
      601 tttcctcaat tgaatatttt tgcaaaaaac gatatcgccg ttgtttatta tatggacatc
      661 ggcgagaatt tcgtttcgaa aggcaattct gcgtcaggtg gtacggagaa gttttacgag
      721 aataagcttg gcggggaagt agacctgtcg aaagattgcg ttgtgagtaa agagcaaatg
      781 attgaagcgg caaagcagtt ttttgctacg aagcaacggc ccgagcaact gacatggagc
      841 gagttggact acaaagacga cgacgacaaa tgagagctcg gctgctaaca aagcccgaaa
      901 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      961 taaacgggtc ttgaggggtt ttttggtcga cgctctccct tatgcgactc ctgcattagg
     1021 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
     1081 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
     1141 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
     1201 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
     1261 aggatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc
     1321 gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg
     1381 catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg
     1441 tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct
     1501 acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac
     1561 ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg
     1621 ataagctgtc aaacatgaga attacaactt atatcgtatg gggctgactt caggtgctac
     1681 atttgaagag ataaattgca ctgaaatcta gaaatatttt atctgattaa taagatgatc
     1741 ttcttgagat cgttttggtc tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt
     1801 gcagggcggt ttttcgaagg ttctctgagc taccaactct ttgaaccgag gtaactggct
     1861 tggaggagcg cagtcaccaa aacttgtcct ttcagtttag ccttaaccgg cgcatgactt
     1921 caagactaac tcctctaaat caattaccag tggctgctgc cagtggtgct tttgcatgtc
     1981 tttccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg gactgaacgg
     2041 ggggttcgtg catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc
     2101 gtggaatgag acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga
     2161 acaggagagc gcacgaggga gccgccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2221 gggtttcgcc accactgatt tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc
     2281 ctatggaaaa acggctttgc cgcggatgta tgctatacga agttatgcga tcgcctctcg
     2341 cctgtcccct cagttcagta atttcctgca tttgcctgtt tccagtcggt agatattcca
     2401 caaaacagca gggaagcagc gcttttccgc tgcataaccc tgcttcgggg tcattatagc
     2461 gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg
     2521 tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc
     2581 acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg
     2641 gaatcctgct ctgcgaggct ggccggctac cgccggcgta acagatgagg gcaagcggcg
     2701 gagaattaca acttatatcg tatggggctg acttcaggtg ctacatttga agagataaat
     2761 tgcactgaaa ccgcggccct ctcacttccc tgttaagtat cttcctggca tcttccagga
     2821 aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg
     2881 agtcagtgag cgaggaagcg gaatatatcc tgtatcacat attctgctga cgcaccggtg
     2941 cagccttttt tctcctgcca catgaagcac ttcactgaca ccctcatcag tgccaacata
     3001 gtaagccagt atacactccg ctagcgctga tgtccggcgg tgcttttgcc gttacgcacc
     3061 accccgtcag tagctgaaca ggagggacag ctgatagaaa cagaagccac tggagcacct
     3121 caaaaacacc atcatacact aaatcagtaa gttggcagca tcacccgacg cactttgcgc
     3181 cgaataaata cctgtgacgg aagatcactt cgcagaataa ataaatcctg gtgtccctgt
     3241 tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag
     3301 aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc
     3361 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
     3421 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
     3481 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
     3541 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
     3601 ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
     3661 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
     3721 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
     3781 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
     3841 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
     3901 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
     3961 tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
     4021 ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac
     4081 gcctggttgc tacgcctgaa taagtgataa taagcggatg aatggcagaa attcgaaagc
     4141 aaattcgacc cggtcgtcgg ttcagggcag ggtcgttaaa tagccgctta tgtctattgc
     4201 tggtttaccg gtttattgac taccggaagc agtgtgaccg tgtgcttctc aaatgcctga
     4261 ggccagtttg ctcaggctct ccccgtggag gtaataattg acgatatgat catttattct
     4321 gcctcccaga gcctgataaa aacggttagc gcttcgttaa tacagatgta ggtgttccac
     4381 agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgcttgtt
     4441 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgct gccggcacct
     4501 gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc
     4561 gcgcccaccg gaaggagcta ccggcagcgg tgcggactgt tgtaactcag aataagaaat
     4621 gaggccgctc atggcgttga ctctcagtca tagtatcgtg gtatcaccgg ttggttccac
     4681 tctctgttgc gggcaacttc agcagcacgt aggggacttc cgcgtttcca gactttacga
     4741 aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca
     4801 gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg
     4861 ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc
     4921 aacgctgccc gactttaaac gtggatcatt ttctttaaat ttatgctgac gacctttgaa
     4981 tttgcctttt ttcttagcaa tttcgattcc ttgtgcctga cgttccttaa ttttttttcg
     5041 ttctgattct gcttgatact tgtacaattc aatgacaagg ctattaatca aacgccttaa
     5101 attttcatct tcaataccat tcattgaggg taaatttaag acttccaggg ttgccccctt
     5161 aatttgaatt tgattcatca attctgttaa ttctttatta tttcgtccta atcgatctaa
     5221 ttcagtaaca ataacaatat ccccttcacg aatatagtta agcatagctt gtaattgtgg
     5281 gcgttcgacc gattgaccgc ttaatttgtc tgaaaagacc ttagaaacgc cctgtaacgc
     5341 ttgtaattgc cgatctaagt tctgttcttt gctactgaca cgtgcataac caattttagc
     5401 cattttcaac caacctctaa aattctctcg gttgcaataa ccaatcagca atatctactt
     5461 tttcaatttc aaattgctta tcagaaattg tcttttcgta agcgataaaa tcttgcgcat
     5521 attgttgctc attaaaaata gccaccactt cgtcattttc taaaactcga taaataaatt
     5581 ttttcatttt actcctccta ttatgcccaa cttaaatgac ctattcacca agtcaattat
     5641 actgctaaaa tcatattagg acaaataggt atactctatt gacctataaa tgatagcaac
     5701 ttaaaagatc aagtgttcgc ttcgctctca ctgcccctcg acgttttagt agcctttccc
     5761 tcacttcgtt cagtccaagc caactaaaag ttttcgggct actctctcct tctcccccta
     5821 ataattaatt aaaatcttac tctgtatatt tctgctaatc attcactaaa cagcaaagaa
     5881 aaacaaacac gtatcataga tataaatgta atggcatagt gcgggtttta ttttcagcct
     5941 gtatcgtagc taaacaaatc gagttgtggg tccgttttgg ggcgttctgc caatttgttt
     6001 agagtttctt gaataaatgt acgttctaaa ttaaacgaag ctgtcagcgc ctttatatag
     6061 ctttctcgtt cttctttttt taatttaatg atcgatagca acaatgattt aacactagca
     6121 agttgaatgc caccatttct tcctggttta atcttaaaga aaatttcctg attcgccttc
     6181 agtaccttca gcaatttatc taatgtccgt tcaggaatgc ctagcacttc tctaatctct
     6241 tttttggtcg tcgctaaata aggcttgtat acatcgcttt tttcgctaat ataagccatt
     6301 aaatcttctt tccattctga caaatgaaca cgttgacgtt cgcttctttt tttcttgaat
     6361 ttaaaccacc cttgacggac aaataaatct ttactggtta aatcacttga tacccaagct
     6421 ttgcaaagaa tggtaatgta ttccctatta gccccttgat agttttctga ataggcactt
     6481 ctaacaattt tgattacttc tttttcttct aagggttgat ctaatcgatt attaaactca
     6541 aacatattat attcgcacgt ttcgattgaa tagcctgaac taaagtaggc taaagagagg
     6601 gtaaacataa cgctattgcg ccctactaaa cccttttctc ctgaaaattt cgtttcgtgc
     6661 aataagagat taaaccaggg ttcatctact tgttttttgc cttctgtacc gcttaaaacc
     6721 gttagacttg aacgagtaaa gcccttatta tctgtttgtt tgaaagacca atcttgccat
     6781 tctttgaaag aataacggta attgggatca aaaaattcta cattgtccgt tcttggtata
     6841 cgagcaatcc caaaatgatt gcacgttaga tcaactggca aagactttcc aaaatattct
     6901 cggatatttt gcgagattat tttggctgct ttgacagatt taaattctga ttttgaagtc
     6961 acatagactg gcgtttctaa aacaaaatat gcttgataac ctttatcaga tttgataatt
     7021 aacgtaggca taaaacctaa atcaatagct gttgttaaaa tatcgcttgc tgaaatagtt
     7081 tctttttccg tgtgaatatc aaaatcaata aagaaggtat tgatttgtct taaattgttt
     7141 tcagaatgtc ctttagtgta tgaacggttt tcgtctgcat acgtaccata acgataaacg
     7201 tttggtgtcc aatgcgtaaa tgtatcttga ttttcgtgaa tcgcttcttc ggaagtcaga
     7261 acaacgccac gtccgccaat catgcttttt tttgagcgat acgcaaaaat agccccttta
     7321 cttttacctg gcttggtagt gattgagcga attttactat ttttaaattt gtactttaac
     7381 aagccgtcat gaagcacagt ttctacaaca aaagggatat tcattcagct gttctccttt
     7441 cttacgaaaa ttaattagtt agaagctacg atcaaagttg aatcacaaca aaaaaggcaa
     7501 tcaactaagt ttttcttaat tgattgcctg gtatcttctt aaagacttga aatcccctca
     7561 aaaacccgat ataatgggtt tacagatatt taagtatctg attaataaag taattaaata
     7621 ctttaccaaa ttttgggtct cgacttcttt aattgattgg tggtaatcaa ttaaggctcg
     7681 cagttaaagt ttctcaggct ttaactggtc gtggctcttt ttttgtattc tttattcagt
     7741 tcgttgtttc gttatatcta gtatatcgct ttttaaaaaa ataagcaatg atttcgtgca
     7801 ttattcacac gaaatcattg cttttttctt cttccatttc taactccaat gttacttgtt
     7861 ctgtttctgg ttctggttct gttggctcat ttgggattaa atccactact agcgttgagt
     7921 tagttccgtc tctaatagcc ggttaagtaa tagccggtta agtggtcaaa ctttgggaaa
     7981 atctcaaccc gcattaagtt ttgatgccat gacaatcgtt ggaaatttga acaaaactaa
     8041 tgctaaaaag ctatctgact ttatgagtgt agagccacaa atacgacttt gggatatact
     8101 tcaaacaaag tttaaagcta aggcacttca agaaaaagtt tatatcgaat atgacaaagt
     8161 aaaagcagat acttgggata gacgtaatat gcgtgttgaa tttaatccca ataaactcac
     8221 acatgaagaa atgatttggt taaaacaaaa tattatcgac tacatggaag atgacggttt
     8281 tacaagatta gacttagctt ttgattttga agatgatttg agcgattact atgcaatgac
     8341 tgataaagca gttaagaaaa ctgtttttta tggtcgtaat ggcaagccag aaacaaaata
     8401 ttttggtgtc cgtgatagtg atagatttat tagaatttat aataaaaaac aagaacgtaa
     8461 agataacgca gatgttgaag ttgtgtctga acatttatgg cgtgtagaag ttgaattaaa
     8521 aagagatatg gttgattact ggaatgattg ttttaatgat ttacacatct tgaaacctgc
     8581 gtgggctact ttagaaaaaa ttaatgagca agctatggtt tatactttgt tgcatgaaga
     8641 aagtatgtgg ggaaagctaa gtaagaatac taagactaaa tttaaaaaat tgattagaga
     8701 aatatctcca attgatttaa cggaattaat gaaatcgact ttaaaagcga acgaaaaaca
     8761 attgcaaaag cagattgatt tttggcaacg tgaatttagg ttttggaagt aaaataagtt
     8821 ttatttgata aaaattgcta attcagtata attaatattt acgaggtgac ataacgtatg
     8881 aaaaaatcag aggattattc ctcctaaata taaaaattta aaatttagga ggaagttata
     8941 tatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaagaat accgctgcgt
     9001 aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaat
     9061 cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga
     9121 aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga
     9181 acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga
     9241 agggtatgaa gatgaacaaa gccctgaaaa gactatcgag ctgtatgcgg agtgcatcag
     9301 gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag acggccgctt
     9361 agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg aaaactggga
     9421 agaagacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga cggaaaagcc
     9481 cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct ttgtgaaaga
     9541 tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca agtggtatga
     9601 cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt atgtcgagct
     9661 attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt atattttact
     9721 ggatgaattg ttttagtacc gagtagtttt tagattttga aagtgaattt aattttatac
     9781 acgtaagtga tcataaaatt tatgaacgta taacaaccac attttttggt tgcttgtggt
     9841 tttgattttg aatttggttt tgaacttatg gactgattta ttcagtccat tttttgtgct
     9901 tgcacaaaaa ctagcctcgc agagcacacg cattaatgac ttatgaaacg tagtaaataa
     9961 gtctagtgtg ttatacttta cttggaagat gcaccgaata aaaaatattg aagaacaact
    10021 agcaaaagat tttaaagagt tattttattt taagtcttta taacatgagt gaagcgaatt
    10081 tttaaatttc gatagaaatt tttacatcaa aaagccccct gtcaaaattg acgaaggggg
    10141 ttttttggcg cacgcttttc gttagaaata tacaagattg aaaatcgtgt ataagtgcgc
    10201 cctttgtttt gaacttagca cgttacatca attttttaaa atgatgtata agtgcgccct
    10261 tttaaatttt gagtgattat attttttgag ttagaaaaag ggattgggaa aatttcccaa
    10321 aataatttaa aaaataagca aaaattttcg atagagaatg tgctattttt tgtcaaaggt
    10381 gtataccttg actgtgcttg ctgttacatt aagtttattt ttaagttatt aaaaaagaaa
    10441 tagcttttaa agtttggctc gctgtcgctt tataaagctg attgactttt gattgcaaac
    10501 tacttaaaga aaacaaactc ggactattcg ttttcttctc tttggtttga acatcagcaa
    10561 ttatcccctc ttgattgcct attttagctt gtttagaaga aacaaaagct aaaagctcct
    10621 cttgggtttt aaaacgctgt gtggggctta gaacgccctt aaacgaccct tggtttactt
    10681 ttatactagc ttccacctcg aaaaaaggtt cttttttaaa attctctatg gcttcctggc
    10741 gctgaaaaat aaggtataag gtgggcgttt gaacacgtcc tagtgaaaat gtaccttgta
    10801 cgcccttctg ttgtaaattt aacgtataca aagggcttgc gttcatgccg atcaaccaat
    10861 cggcaatttg gcgtgtttgc gcttcttgat aaaagggata gtaattcatt ccaggttgca
    10921 aattttgaaa accgcttcgg attacatctt tttctaagct attgatccat agtcttttaa
    10981 atgttttatc ttttgaaaag gcatttgctt tatggataat cgaccaggcg atattttcac
    11041 cttctctgtc gctatctgtt gcaacaataa ttgtatttgc ctttttgaga agttctgcaa
    11101 caattttaaa ctgctttccc ttatcttttg caacttcaaa atcgtatcga tcaggaaaaa
    11161 tcggcaaaga ttcaagtttc caattttgcc acttttcgtc ataatgacct ggttctgcta
    11221 attccactaa atgcccaaaa ccaaaggtga taaacgtttc atctgtaaat agtgggtctt
    11281 tgatctcaaa ataaccgtct tttttggtgc tttgttttaa agcacttgcg taggctaatg
    11341 cctggcttgg tttttcagct aaaataaccg tactcattaa ctatccctct tttcattgtt
    11401 ttttctttga tcgactgtca cgttatatct tgctcgatac cttctaaacg ttcggcgatt
    11461 gattccagtt tgttcttcaa cttctttatc ggataaacca ttcaaaaaca aatcgaaagc
    11521 cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg
    11581 gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt
    11641 gaatccgtta gcgaggtgcc gccggcttcc attca
//
