LOCUS       sequence-367526-       10032 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9 plasmid to express the Cas9 in OSS cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10032)
  AUTHORS   Meng Q, Stoyko D, Andrews CM, Konstantinidou P, Genzor P, O T, 
            Elchert AR, Benner L, Sobti S, Katz EY, Haase AD
  TITLE     Functional editing of endogenous genes through rapid selection of 
            cell pools (Rapid generation of endogenously tagged genes in 
            Drosophila ovarian somatic sheath cells).
  JOURNAL   Nucleic Acids Res. 2022 May 27. pii: 6594084. doi: 
            10.1093/nar/gkac448.
  PUBMED    35639929
REFERENCE   2  (bases 1 to 10032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2022 May 27. pii: 6594084. doi: 10.1093/nar/gkac448."
COMMENT     Sequence Label: Cas9_nonPuro_OSS
FEATURES             Location/Qualifiers
     source          1..10032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1579..1644
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1651..1671
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1696..5796
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1696..5796
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5797..5844
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5860..5913
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    5945..6079
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5969..5988)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6023..6042
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6680..7268)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6760..6779)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7439..8230,8231..8299))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8062..8081
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8300..8404)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8472..8490
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_RNA        complement(8642..8717)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(8736..9135)
                     /label=dU6-2 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-2 
                     snRNA (Das et al., 1987)"
ORIGIN
        1 tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg ctgcgctctc
       61 tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt ccttttatgc
      121 gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga aaacgctaaa
      181 ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac gatagcaaca
      241 aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc gcgtgcgagc
      301 aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag ttttggcgaa
      361 aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat ttaatattgt
      421 ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat tatttgaaag
      481 agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta ctgagtcaca
      541 gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag cagcttagac
      601 cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa cgcatagact
      661 gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat tactattcaa
      721 aactattaag atagcaatca cattcaatag ccaaatacta taccacctga gcgatgcaac
      781 gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta tagaaatgct
      841 tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt aaaaccggct
      901 taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg tgttgcacaa
      961 aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc taatatgaat
     1021 attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca ctataaacac
     1081 atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt gtaaaagcac
     1141 cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta acccccaaat
     1201 tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg cgatatacaa
     1261 gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg aaaacccaat
     1321 cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga gagcggagag
     1381 cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct gcgggaccag
     1441 ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc gacacaaagc
     1501 cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac ctactagtcc
     1561 agtgtggtgg aattcatgga ctataaggac cacgacggag actacaagga tcatgatatt
     1621 gattacaaag acgatgacga taagatggcc ccaaagaaga agcggaaggt cggtatccac
     1681 ggagtcccag cagccgacaa gaagtacagc atcggcctgg acatcggcac caactctgtg
     1741 ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc
     1801 aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc
     1861 gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag
     1921 aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc
     1981 ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac
     2041 cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac
     2101 cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg
     2161 gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga cctgaacccc
     2221 gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc
     2281 gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg
     2341 agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc
     2401 ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc
     2461 gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac
     2521 aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc caagaacctg
     2581 tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac caaggccccc
     2641 ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac cctgctgaaa
     2701 gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag
     2761 aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta caagttcatc
     2821 aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaacagagag
     2881 gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg
     2941 ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac
     3001 cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc
     3061 aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg
     3121 aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc
     3181 aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag
     3241 tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag
     3301 cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac
     3361 cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac
     3421 tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat
     3481 ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg
     3541 gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg
     3601 aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac
     3661 accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc
     3721 aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg
     3781 atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag
     3841 ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc
     3901 atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc
     3961 gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac
     4021 agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg
     4081 aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg
     4141 cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac
     4201 gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg
     4261 ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg
     4321 aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag
     4381 ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc
     4441 atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac
     4501 tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc
     4561 accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc
     4621 gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc
     4681 ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac
     4741 gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac
     4801 ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag
     4861 atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag
     4921 ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa
     4981 aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc
     5041 gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc
     5101 cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa
     5161 ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag
     5221 aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc
     5281 aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct
     5341 gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg
     5401 tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag
     5461 ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc
     5521 tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag
     5581 caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc
     5641 aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac
     5701 accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac
     5761 gagacacgga tcgacctgtc tcagctggga ggcgacaaaa ggccggcggc cacgaaaaag
     5821 gccggccagg caaaaaagaa aaagggatca ggcaagcttg agggcagagg aagtcttcta
     5881 acatgcggtg acgtggagga gaatcccggc cctgctagaa tacgggcggc cgcagtgatc
     5941 gagggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     6001 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     6061 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     6121 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     6181 tgatcagtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     6241 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     6301 aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
     6361 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
     6421 ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg
     6481 acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta
     6541 tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc
     6601 aggtagatga cgaccatcag ggacagcttc aaggccagca aaaggccagg aaccgtaaaa
     6661 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6721 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6781 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6841 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6901 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6961 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7021 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7081 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     7141 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7201 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7261 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7321 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7381 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7441 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7501 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7561 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7621 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7681 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7741 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7801 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7861 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7921 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7981 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8041 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8101 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8161 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8221 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8281 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8341 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8401 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     8461 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaatta attctatatt
     8521 ctaaaaacac aaatgatact tctaaaaaaa aatcatgaat ggcatcaact ctgaatcaaa
     8581 tctttgcaga tgcacctact tctcatttcc actgtcacat catttttcca gatctaaaaa
     8641 agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt ttaacttgct
     8701 atttctagct ctaaaacgga agagctcgct cttccgaagt attgaggaaa acatacctat
     8761 ataaatgatc aacatcagga aagagcagtt gagaattata agaattggca aatggtctta
     8821 agaaccctct gcttaagatt ttcaaaattt cctttagaat caaagtgtcc tattgttcgt
     8881 ttgttgaaaa cacagttcga tttattgact ataataaatt gatagtttta aatatagagg
     8941 cacgactaag agagtttggt tttgttttgg ttagggacca aaaaaagtat acataacgaa
     9001 taaaaaagga tttaagtgca aatggtaaaa aagtgtcgag tttttcttga gttgattgtg
     9061 ctgtaatgag actctgcatt cggcatttga ctcggctttt cctactcgtg ccgtatttca
     9121 ggctgcaagt cgaacagatc tcgctgcctg ttatgtggcc cacaaaccaa gacacgtttt
     9181 atggccatta aagctggctg atcgtcgcca aacaccaaat acataatgaa tatgtacaca
     9241 ttcgagaaag aagcgatcaa agaagcgtct tcgggcggag taggagaatg cggaggagaa
     9301 ggagaacgag ctgatctagt atctctccac aatccaatgc caactgacca actggccata
     9361 ttcggagcaa tttgaagcca atttccatcg cctggcgatc gctccattct tggctatatg
     9421 tttttcaccg ttacccgggg ccattttcaa agactcgtcg gcaagataag attgtgtcac
     9481 tcgctgtctc tcttcatttg tcgaagaatg ctgaggaatt tcgcgatgac gtcggcgagt
     9541 attttgaaga atgagaataa tttgtattta tacgaaaatc agttagtgga attttctaca
     9601 aaaacatgtt atctatagat aattttgttg caaaatatgt tgactatgac aaagattgta
     9661 tgtatatctt taatgtattc tcattttctt atgtatttat aatggcaatg atgatactga
     9721 tgatatttta agatgatgcc agaccaaaag gcttgaattt ctgcgtcttt tgccgaacgc
     9781 agtgcatgtg caattgttgt tttttggaat attcaatttt cggactgtcc gctttgattt
     9841 cagtttcttg gcttattcaa aaagcaaagt aaagccaaaa aagcgagatg gcaataccaa
     9901 atgcggcaaa acggtagtgg aaggaaaggg gtgcggggca gcggaaggaa gggtggggcg
     9961 gggcgtggcg gggtctgtgg ctgggcgcga cgtcaccgac gttggagcca ctcctttgac
    10021 catgtgtgcg tg
//
