LOCUS       sequence-36753-e         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 2308 (=HL 716 + pHL 814)
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(53..70)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      223..309
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      402..429
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    496..514
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ctctcaataa aagctagctt ggctgttttg gcggatgaga gaagattttc agcctgatac
       61 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg
      121 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta
      181 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      241 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      301 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg aggggtggcg
      361 ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga
      421 tggccttttt gcgtttctac aaactctttt gtttattttt ctaaatacat tcaaatatgt
      481 atccgctcag gatcctccct atcagtgata gagattgaca tccctatcan tgatanagat
      541 actcccgggt tatatgcctt tattgtcaca gattttattt tctgttgggc cattgcattg
      601 ccactgattt tccaacatat aaaaagacaa gcccgaacag tcgtccgggc ttttttttta
//
