LOCUS       sequence-367534-       10362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression of Nup1 C-terminal truncation (nup1-21 allele).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10362)
  AUTHORS   Bogerd AM, Hoffman JA, Amberg DC, Fink GR, Davis LI
  TITLE     nup1 mutants exhibit pleiotropic defects in nuclear pore complex 
            function.
  JOURNAL   J Cell Biol. 1994 Oct . 127(2):319-32.
  PUBMED    7929578
REFERENCE   2  (bases 1 to 10362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 1994 Oct . 127(2):319-32."
COMMENT     Sequence Label: LDB34
FEATURES             Location/Qualifiers
     source          1..10362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        191..471
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             472..1146
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1249..1704)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1386..1407)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1598..1617
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1830..1852
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1844..1861
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1845..1861
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1868..1887
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1868..1886
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1919..1935
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1919..1935
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(7589..7607)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7628..7644)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7628..7644)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7641..7663)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7652..7668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7676..7682,7683..7700,7701..7706))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7721..7742
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7859..7876)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8030..8618)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8110..8129)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8789..9580,9581..9649))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9412..9431
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9650..9754)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    9791..10294
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     10335..10353
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatagatc acgacattac tatatatata atataggaag catttaatag aacagcatcg
      241 taatatatgt gtactttgca gttatgacgc cagatggcag tagtggaaga tattctttat
      301 tgaaaaatag cttgtcacct tacgtacaat cttgatccgg agcttttctt tttttgccga
      361 ttaagaatta attcggtcga aaaaagaaaa ggagagggcc aagagggagg gcattggtga
      421 ctattgagca cgtgagtata cgtgattaag cacacaaagg cagcttggag tatgtctgtt
      481 attaatttca caggtagttc tggtccattg gtgaaagttt gcggcttgca gagcacagag
      541 gccgcagaat gtgctctaga ttccgatgct gacttgctgg gtattatatg tgtgcccaat
      601 agaaagagaa caattgaccc ggttattgca aggaaaattt caagtcttgt aaaagcatat
      661 aaaaatagtt caggcactcc gaaatacttg gttggcgtgt ttcgtaatca acctaaggag
      721 gatgttttgg ctctggtcaa tgattacggc attgatatcg tccaactgca tggagatgag
      781 tcgtggcaag aataccaaga gttcctcggt ttgccagtta ttaaaagact cgtatttcca
      841 aaagactgca acatactact cagtgcagct tcacagaaac ctcattcgtt tattcccttg
      901 tttgattcag aagcaggtgg gacaggtgaa cttttggatt ggaactcgat ttctgactgg
      961 gttggaaggc aagagagccc cgaaagctta cattttatgt tagctggtgg actgacgcca
     1021 gaaaatgttg gtgatgcgct tagattaaat ggcgttattg gtgttgatgt aagcggaggt
     1081 gtggagacaa atggtgtaaa agactctaac aaaatagcaa atttcgtcaa aaatgctaag
     1141 aaataggtta ttactgagta gtatttattt aagtattgtt tgtgcacttg ccgatctatg
     1201 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg
     1261 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     1321 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     1381 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     1441 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     1501 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     1561 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     1621 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     1681 ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg
     1741 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     1801 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     1861 tgaattgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc
     1921 tagaactagt ggatccaagc ttatcgattt cgaacccggg gtaccgaatt catcagtgaa
     1981 ctcttcatca ttcaaaaaca cccaatcata gttgaacttg gagttaaatc tatcttccac
     2041 ggatttaata gactcagcca acgaatatag atctctatta cgcactagag tgacaaaagt
     2101 ggccttttcc cgaggaccgg tatactttgg tagttccact ttcgagtatt tatagcccga
     2161 gccggaagat agcacggtac tgtactttgg gcttggggca gagcctctcg catactgagc
     2221 tccatgaaag aacacataca cagtaaacac ggcgaccaga agaagtccta attttttgta
     2281 aacaggctgc ttgctagctg ggatcataat cttcgccatt ttggttattg actctatccc
     2341 ttaaaaactc ttctgatgga gtactttact tcgattgctt aacgaaatct ttgtgaggaa
     2401 aaagatatct cttaaaatta gaaagtacaa tagtctagcg tattatacta agaatctgca
     2461 aaaaagaagc aagaaggcac cacctattat agacctttga cacgaagtct ctctggagtg
     2521 ctttggccta cgtgtgcggt actcgtttac atgggacaac cacggttttt tttttggtgt
     2581 tactggaggt atacagtgcg tatatccact tgtacgacaa gagatttaca ctacaccgcg
     2641 taaagaaaac gccgacacca aatataagtc acgtgtatgc aaagcctatt ttatgcccta
     2701 attttcaagc cccggttttt acgccctagt ttttatattt agggtttgtc gttgcacgtg
     2761 atcaatggtt cgtattatgt gacattgaaa tgctttttca ttttaatttt tttctttgac
     2821 gaaatttcgt aatgtcaaga aacacttaaa gaaaataagt gatgaggaac tcaataagga
     2881 cactacgtag cggtgcaaat acgataggat attagcctcg aaagggttat agggacagag
     2941 agtgagcgac aatttttagt cattcatgtc ttcaaacact tcttctgtga tgtcttctcc
     3001 acgtgtcgaa aagagatcgt tttcttccac tttaaaatca ttcttcacaa accccaataa
     3061 aaaacggcca tcgagcaaga aagttttcag ttcaaacctc tcatacgcga atcatttgga
     3121 ggaatcagat gttgaagaca cactgcatgt taataagaga aagagggtgt ccggtacatc
     3181 acagcatagc gacagcttaa ctcagaacaa caataatgcg ccaattataa tatatggaac
     3241 cgaaaacact gagagaccgc cgcttttgcc aattttgccc attcaaagac tgaggttatt
     3301 aagggagaag cagagggtga gaaatatgcg tgagcttgga ttaattcaat caactgaatt
     3361 tccatctatt acatcgtcgg ttatattggg ctctcaaagt aaaagcgatg agggaggatc
     3421 gtacctatgc acatcatcta ctccttcccc tattaaaaac ggttcttgca ctaggcagtt
     3481 ggccggaaaa agcggtgaag acaccaatgt tggactaccc attctcaaat cattgaaaaa
     3541 tagatccaat agaaaaaggt ttcatagtca gtcaaagggg accgtgtggt cagcaaattt
     3601 tgaatatgat ttgtcagaat atgacgctat acaaaaaaag gataacaagg ataaggaagg
     3661 taacgctggc ggtgatcaga agacaagcga gaatagaaat aatattaaga gtagtatttc
     3721 aaatggcaat ctggctacag gccctaacct gacaagcgaa attgaagacc tacgtgcaga
     3781 catcaactct aataggttat cgaatcctca aaaaaatcta cttttaaaag gaccagcttc
     3841 cacagttgca aaaactgccc ctattcagga gagctttgtt cccaattcag agcgctctgg
     3901 tacgcctacg ttaaagaaaa atattgagcc caaaaaggac aaagaaagta ttgttttgcc
     3961 caccgtaggt tttgacttta tcaaggacaa tgagactcca tctaagaaaa cttctcctaa
     4021 ggcaacttct tctgcaggtg cagtctttaa atcgagtgta gaaatgggaa aaaccgataa
     4081 gtcaacgaaa actgccgagg cgcctacctt atcattcaat tttagccaaa aggctaataa
     4141 aactaaggct gtcgacaata ctgtcccttc cacaacttta ttcaattttg gtggtaaatc
     4201 agataccgtt acttctgcca gtcaaccttt taaatttgga aagacatccg aaaaaagtga
     4261 aaatcataca gaatcagacg cgcctccaaa atcaactgct ccaatatttt cttttggtaa
     4321 acaagaagag aatggtgatg aaggtgatga tgaaaatgag cccaaaagaa aaaggcgttt
     4381 acctgttagc gaggatacaa acaccaagcc tttattcgat ttcggcaaga ccggtgatca
     4441 aaaggagacc aaaaagggag agtcagaaaa ggacgcatca gggaaaccaa gctttgtctt
     4501 tggtgcaagt gataagcaag ctgaaggtac accattattt acattcggaa aaaaagctga
     4561 tgtaacaagc aatattgact cctctgcaca atttaccttt ggtaaagccg ccaccgcgaa
     4621 agaaacacac accaaaccat ctgagacacc tgccacaata gtcaagaagc ctacttttac
     4681 ttttgggcag tcaacaagtg aaaataagat ctctgaggga agtgcgaaac ctacattctc
     4741 tttctctaag tcagaggagg aacgtaagag tagtccaatt tcaaacgaag cagctaaacc
     4801 ctcgttttcg tttccgggca agcctgttga tgttcaagca ccgacggatg ataagactct
     4861 caagccaact ttttctttta ctgaacctgc tcaaaaagat tcatctgttg tttcggaacc
     4921 taaaaagccc tcctttacgt ttgcgtcttc aaaaacctca caaccaaagc cattgttttc
     4981 atttggtaag tcagatgcag ctaaagaacc accaggctct aacacctcat tttctttcac
     5041 taaacctcct gctaatgaga cagataaaag acctacaccg ccatctttca cctttggcgg
     5101 ttccacaaca aataatacaa caaccactag cacaaaacca tcttttagtt ttggggctcc
     5161 cgagtcgatg aagtcgacag caagtacagc ggcagcaaat acggagaagc tatcaaatgg
     5221 cttttccttt acaaagttca atcacaataa agaaaagtca aactctccaa cttctttctt
     5281 cgatggttct gcttcctcaa cgccgattcc tgtcttgggt aagccaacag acgctactgg
     5341 taatacaaca tctaaatctg cattttcatt cggtactgct aacaccaatg gtaccaatgc
     5401 ctcagcaaac tccacatcat tctcgtttaa cgcccctgct actggtaacg gcacaactac
     5461 tacttccaat acctcaggaa ccaatatagc gggtacattt aacgtaggaa aaccggatca
     5521 aagtatcgct agtggcaata cgaatggagc gggctcggca tttggctttt cgagctcagg
     5581 aacagcagca actggtgcag cttctaatca atcttcattt aattttggaa acaatggtgc
     5641 agggggtctc aatcctttta catcagcaac ttcgtcaact aatgctaatg ctggtttatt
     5701 caataaacct ccttccacga atgcacaaaa tgtcaatgtt ccctctgctt ttaattttac
     5761 gggaaataat tcaacgcctg gtggcggctc tgtatttaat atgaacggca acactaatgc
     5821 taatacggtg tttgccggct ctaataacca accacatcaa tcgcaaaccc catctttcaa
     5881 tacaaacagc tcattcacgc catcaacagt tcctaatatt aattttagcg gattgaatgg
     5941 cggaattact aataccgcga ccaatgcatt aaggccaagt gatatatttg gtgcgaatgc
     6001 tgcctctggt tccaattcaa acgtaacaaa tccatcatcc atttttgggg gggcaggtgg
     6061 tgtgccgaca acttcttttg ggcagccgct gaaggatata tattcctata ttccttcaac
     6121 ttatacactg catatgaaac ttcttgagaa gttttaaata attggtcttt tttttaatcg
     6181 ggtacaggcg agataatgga tacctgttct agggtaggca aatttggagg caagagccca
     6241 aaattgtttt gttttgacct ggtttctctc agttaacata ttatgaatag catcttacaa
     6301 ataatttacg agtgaaactt tcgatctgca aagcgctcct tttttctttc cgaaaaaata
     6361 cagtaaaata aggtctagta cacagcctga tctacaatta gtgttctttt ctgtttactt
     6421 cgatcagctg tttagctttg ccacttttct agtagctcca cacgctgtga aagtaacgag
     6481 acatagaagt cacggatgga tttgaaaagg gattggttac gatggaaaat tacaataggt
     6541 agtgggcctg gcagtattgt gctggatttc ccatcatttt tggtagggtg cgtattcaca
     6601 acaatgatgg ggccaatatt acagaagctt ataggtaaac ttttggtagg tttaataacg
     6661 gtatgcaaat tcttagttat tattggaagc attgtcttcg ttatcggagt tgcatcaaag
     6721 aaatacacat atgacgattt caaagtaagt ataaaaagat caggagagcc aggggaatca
     6781 catgatatga ggactgaacc aaaaagaact gcgaaaacgg caactgttcc aatggagaaa
     6841 gacgagggcg taggttctta taattatttt gaaataccta taacgaaaga aacatctaca
     6901 attccttata ttaattgtga tggcacgagc tcacttagaa agccacccaa cggtccatca
     6961 agcgtaggct tatctaactc caacagatac gaaaatttta taaacatggc acgacataaa
     7021 taaatgtttg tatggcaacc tgtcgattta gcggcaatca tacggcggtg aaaaagtatg
     7081 taactatgca tagttgtcat aaaatttatt tgattcagat ctttgcaaaa agtatttttt
     7141 ttggaatttg gagtttttat tatcttattg tgctataaaa taaacaaagt tgttactaac
     7201 taataataaa gatttagata ttattatatt aactaatgag tttcacttgg aatttcgaaa
     7261 acaatttgct taccagtcaa tttgttgtaa acagcttgga aagattccaa tttgtagtcg
     7321 atttgttgga catcctttga gtctaacaaa accttttgga tcttgttacc accaaccaaa
     7381 tatctaactc tcttaccaac gatttcagtt gggaagacta agtcttccaa gatcttgtca
     7441 tgaacagcag tcaaggttct ggatcttggt ctcttttgga cttgtctaga tgttctagat
     7501 ggctttggca agattcttct ttcagccaag aagatgacat gacggtcttg gaattcctcg
     7561 agggggggcc cggtaccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt
     7621 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7681 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7741 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7801 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7861 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7921 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7981 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8041 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8101 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8161 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8221 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8281 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8341 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8401 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8461 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8521 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8581 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8641 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8701 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8761 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8821 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8881 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8941 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9001 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9061 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9121 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9181 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9241 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9301 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9361 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9421 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9481 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9541 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9601 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9661 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9721 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9781 ggtccttttc atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata
     9841 taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa
     9901 gatgtaaaag actctagggg gatcgccaac aaatactacc ttttatcttg ctcttcctgc
     9961 tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat
    10021 cctgtgattt tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc
    10081 ttgtctaata aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt
    10141 attttttttt cttcattccg taactcttct accttcttta tttactttct aaaatccaaa
    10201 tacaaaacat aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag
    10261 atacgaggcg cgtgtaagtt acaggcaagc gatccgtcta agaaaccatt attatcatga
    10321 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
