LOCUS       sequence-367548-       10605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NUP1 CEN URA3 vector for synthetic lethal sectoring assays and 
            screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Belanger KD, Kenna MA, Wei S, Davis LI
  TITLE     Genetic and physical interactions between Srp1p and nuclear pore 
            complex proteins Nup1p and Nup2p.
  JOURNAL   J Cell Biol. 1994 Aug;126(3):619-30. doi: 10.1083/jcb.126.3.619.
  PUBMED    8045927
REFERENCE   2  (bases 1 to 10605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb.126.3";
            journalName: "J Cell Biol"; date: "1994-08"; volume: "126"; issue: 
            "3"; pages: "619-30"
COMMENT     Sequence Label: LDB73
FEATURES             Location/Qualifiers
     source          1..10605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        194..409
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             410..1213
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1344..1799)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1481..1502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1693..1712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1925..1947
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1939..1956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1940..1956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1963..1982
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1963..1981
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2014..2030
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2014..2030
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(7832..7850)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7871..7887)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7871..7887)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7884..7906)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7895..7911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7919..7925,7926..7943,7944..7949))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7964..7985
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8102..8119)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8273..8861)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8353..8372)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9032..9823,9824..9892))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9655..9674
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9893..9997)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    10034..10537
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     10578..10596
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
      421 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
      481 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
      541 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
      601 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
      661 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
      721 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
      781 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
      841 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
      901 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
      961 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     1021 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     1081 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     1141 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     1201 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     1261 agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg cacagatgcg
     1321 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     1381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1681 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1741 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1801 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     1861 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     1921 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag
     1981 ggcgaattgg agctccaccg cggtggcggc cgctctagaa ctagtggatc ggatccaagc
     2041 ttatcgattt cgaacccggg gtaccgaatt catcagtgaa ctcttcatca ttcaaaaaca
     2101 cccaatcata gttgaacttg gagttaaatc tatcttccac ggatttaata gactcagcca
     2161 acgaatatag atctctatta cgcactagag tgacaaaagt ggccttttcc cgaggaccgg
     2221 tatactttgg tagttccact ttcgagtatt tatagcccga gccggaagat agcacggtac
     2281 tgtactttgg gcttggggca gagcctctcg catactgagc tccatgaaag aacacataca
     2341 cagtaaacac ggcgaccaga agaagtccta attttttgta aacaggctgc ttgctagctg
     2401 ggatcataat cttcgccatt ttggttattg actctatccc ttaaaaactc ttctgatgga
     2461 gtactttact tcgattgctt aacgaaatct ttgtgaggaa aaagatatct cttaaaatta
     2521 gaaagtacaa tagtctagcg tattatacta agaatctgca aaaaagaagc aagaaggcac
     2581 cacctattat agacctttga cacgaagtct ctctggagtg ctttggccta cgtgtgcggt
     2641 actcgtttac atgggacaac cacggttttt tttttggtgt tactggaggt atacagtgcg
     2701 tatatccact tgtacgacaa gagatttaca ctacaccgcg taaagaaaac gccgacacca
     2761 aatataagtc acgtgtatgc aaagcctatt ttatgcccta attttcaagc cccggttttt
     2821 acgccctagt ttttatattt agggtttgtc gttgcacgtg atcaatggtt cgtattatgt
     2881 gacattgaaa tgctttttca ttttaatttt tttctttgac gaaatttcgt aatgtcaaga
     2941 aacacttaaa gaaaataagt gatgaggaac tcaataagga cactacgtag cggtgcaaat
     3001 acgataggat attagcctcg aaagggttat agggacagag agtgagcgac aatttttagt
     3061 cattcatgtc ttcaaacact tcttctgtga tgtcttctcc acgtgtcgaa aagagatcgt
     3121 tttcttccac tttaaaatca ttcttcacaa accccaataa aaaacggcca tcgagcaaga
     3181 aagttttcag ttcaaacctc tcatacgcga atcatttgga ggaatcagat gttgaagaca
     3241 cactgcatgt taataagaga aagagggtgt ccggtacatc acagcatagc gacagcttaa
     3301 ctcagaacaa caataatgcg ccaattataa tatatggaac cgaaaacact gagagaccgc
     3361 cgcttttgcc aattttgccc attcaaagac tgaggttatt aagggagaag cagagggtga
     3421 gaaatatgcg tgagcttgga ttaattcaat caactgaatt tccatctatt acatcgtcgg
     3481 ttatattggg ctctcaaagt aaaagcgatg agggaggatc gtacctatgc acatcatcta
     3541 ctccttcccc tattaaaaac ggttcttgca ctaggcagtt ggccggaaaa agcggtgaag
     3601 acaccaatgt tggactaccc attctcaaat cattgaaaaa tagatccaat agaaaaaggt
     3661 ttcatagtca gtcaaagggg accgtgtggt cagcaaattt tgaatatgat ttgtcagaat
     3721 atgacgctat acaaaaaaag gataacaagg ataaggaagg taacgctggc ggtgatcaga
     3781 agacaagcga gaatagaaat aatattaaga gtagtatttc aaatggcaat ctggctacag
     3841 gccctaacct gacaagcgaa attgaagacc tacgtgcaga catcaactct aataggttat
     3901 cgaatcctca aaaaaatcta cttttaaaag gaccagcttc cacagttgca aaaactgccc
     3961 ctattcagga gagctttgtt cccaattcag agcgctctgg tacgcctacg ttaaagaaaa
     4021 atattgagcc caaaaaggac aaagaaagta ttgttttgcc caccgtaggt tttgacttta
     4081 tcaaggacaa tgagactcca tctaagaaaa cttctcctaa ggcaacttct tctgcaggtg
     4141 cagtctttaa atcgagtgta gaaatgggaa aaaccgataa gtcaacgaaa actgccgagg
     4201 cgcctacctt atcattcaat tttagccaaa aggctaataa aactaaggct gtcgacaata
     4261 ctgtcccttc cacaacttta ttcaattttg gtggtaaatc agataccgtt acttctgcca
     4321 gtcaaccttt taaatttgga aagacatccg aaaaaagtga aaatcataca gaatcagacg
     4381 cgcctccaaa atcaactgct ccaatatttt cttttggtaa acaagaagag aatggtgatg
     4441 aaggtgatga tgaaaatgag cccaaaagaa aaaggcgttt acctgttagc gaggatacaa
     4501 acaccaagcc tttattcgat ttcggcaaga ccggtgatca aaaggagacc aaaaagggag
     4561 agtcagaaaa ggacgcatca gggaaaccaa gctttgtctt tggtgcaagt gataagcaag
     4621 ctgaaggtac accattattt acattcggaa aaaaagctga tgtaacaagc aatattgact
     4681 cctctgcaca atttaccttt ggtaaagccg ccaccgcgaa agaaacacac accaaaccat
     4741 ctgagacacc tgccacaata gtcaagaagc ctacttttac ttttgggcag tcaacaagtg
     4801 aaaataagat ctctgaggga agtgcgaaac ctacattctc tttctctaag tcagaggagg
     4861 aacgtaagag tagtccaatt tcaaacgaag cagctaaacc ctcgttttcg tttccgggca
     4921 agcctgttga tgttcaagca ccgacggatg ataagactct caagccaact ttttctttta
     4981 ctgaacctgc tcaaaaagat tcatctgttg tttcggaacc taaaaagccc tcctttacgt
     5041 ttgcgtcttc aaaaacctca caaccaaagc cattgttttc atttggtaag tcagatgcag
     5101 ctaaagaacc accaggctct aacacctcat tttctttcac taaacctcct gctaatgaga
     5161 cagataaaag acctacaccg ccatctttca cctttggcgg ttccacaaca aataatacaa
     5221 caaccactag cacaaaacca tcttttagtt ttggggctcc cgagtcgatg aagtcgacag
     5281 caagtacagc ggcagcaaat acggagaagc tatcaaatgg cttttccttt acaaagttca
     5341 atcacaataa agaaaagtca aactctccaa cttctttctt cgatggttct gcttcctcaa
     5401 cgccgattcc tgtcttgggt aagccaacag acgctactgg taatacaaca tctaaatctg
     5461 cattttcatt cggtactgct aacaccaatg gtaccaatgc ctcagcaaac tccacatcat
     5521 tctcgtttaa cgcccctgct actggtaacg gcacaactac tacttccaat acctcaggaa
     5581 ccaatatagc gggtacattt aacgtaggaa aaccggatca aagtatcgct agtggcaata
     5641 cgaatggagc gggctcggca tttggctttt cgagctcagg aacagcagca actggtgcag
     5701 cttctaatca atcttcattt aattttggaa acaatggtgc agggggtctc aatcctttta
     5761 catcagcaac ttcgtcaact aatgctaatg ctggtttatt caataaacct ccttccacga
     5821 atgcacaaaa tgtcaatgtt ccctctgctt ttaattttac gggaaataat tcaacgcctg
     5881 gtggcggctc tgtatttaat atgaacggca acactaatgc taatacggtg tttgccggct
     5941 ctaataacca accacatcaa tcgcaaaccc catctttcaa tacaaacagc tcattcacgc
     6001 catcaacagt tcctaatatt aattttagcg gattgaatgg cggaattact aataccgcga
     6061 ccaatgcatt aaggccaagt gatatatttg gtgcgaatgc tgcctctggt tccaattcaa
     6121 acgtaacaaa tccatcatcc atttttgggg gggcaggtgg tgtgccgaca acttcttttg
     6181 ggcagccgca gtcagcccct aatcagatgg ggatgggaac aaataatggc atgagcatgg
     6241 gcggtggtgt tatggcgaac agaaagattg caagaatgag gcactctaaa aggtaaatat
     6301 cggttatgta attaggtatt gtgttgcttt tctgaaggat atatattcct atattccttc
     6361 aacttataca ctgcatatga aacttcttga gaagttttaa ataattggtc ttttttttaa
     6421 tcgggtacag gcgagataat ggatacctgt tctagggtag gcaaatttgg aggcaagagc
     6481 ccaaaattgt tttgttttga cctggtttct ctcagttaac atattatgaa tagcatctta
     6541 caaataattt acgagcgaaa ctttcgatct gcaaagcgct ccttttttct ttccgaaaaa
     6601 atacagtaaa ataaggtcta gtacacagcc tgatctacaa ttagtgttct tttctgttta
     6661 cttcgatcag ctgtttagct ttgccacttt tctagtagct ccacacgctg tgaaagtaac
     6721 gagacataga agtcacggat ggatttgaaa agggattggt tacgatggaa aattacaata
     6781 ggtagtgggc ctggcagtat tgtgctggat ttcccatcat ttttggtagg gtgcgtattc
     6841 acaacaatga tggggccaat attacagaag cttataggta aacttttggt aggtttaata
     6901 acggtatgca aattcttagt tattattgga agcattgtct tcgttatcgg agttgcatca
     6961 aagaaataca catatgacga tttcaaagta agtataaaaa gatcaggaga gccaggggaa
     7021 tcacatgata tgaggactga accaaaaaga actgcgaaaa cggcaactgt tccaatggag
     7081 aaagacgagg gcgtaggttc ttataattat tttgaaatac ctataacgaa agaaacatct
     7141 acaattcctt atattaattg tgatggcacg agctcactta gaaagccacc caacggtcca
     7201 tcaagcgtag gcttatctaa ctccaacaga tacgaaaatt ttataaacat ggcacgacat
     7261 aaataaatgt ttgtatggca acctgtcgat ttagcggcaa tcatacggcg gtgaaaaagt
     7321 atgtaactat gcatagttgt cataaaattt atttgattca gatctttgca aaaagtattt
     7381 tttttggaat ttggagtttt tattatctta ttgtgctata aaataaacaa agttgttact
     7441 aactaataat aaagatttag atattattat attaactaat gagtttcact tggaatttcg
     7501 aaaacaattt gcttaccagt caatttgttg taaacagctt ggaaagattc caatttgtag
     7561 tcgatttgtt ggacatcctt tgagtctaac aaaacctttt ggatcttgtt accaccaacc
     7621 aaatatctaa ctctcttacc aacgatttca gttgggaaga ctaagtcttc caagatcttg
     7681 tcatgaacag cagtcaaggt tctggatctt ggtctctttt ggacttgtct agatgttcta
     7741 gatggctttg gcaagattct tctttcagcc aagaagatga catgacggtc ttggaattcc
     7801 tcgagggggg gcccggtacc agcttttgtt ccctttagtg agggttaatt tcgagcttgg
     7861 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7921 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7981 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     8041 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     8101 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     8161 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8221 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8281 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8341 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8401 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8461 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8521 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8581 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8641 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8701 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8761 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8821 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8881 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8941 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9001 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9061 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9121 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9181 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9241 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9301 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9361 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9421 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9481 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9541 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9601 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9661 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9721 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9781 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9841 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9901 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9961 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10021 ctgggtcctt ttcatcacgt gctataaaaa taattataat ttaaattttt taatataaat
    10081 atataaatta aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt tttgttttcc
    10141 gaagatgtaa aagactctag ggggatcgcc aacaaatact accttttatc ttgctcttcc
    10201 tgctctcagg tattaatgcc gaattgtttc atcttgtctg tgtagaagac cacacacgaa
    10261 aatcctgtga ttttacattt tacttatcgt taatcgaatg tatatctatt taatctgctt
    10321 ttcttgtcta ataaatatat atgtaaagta cgctttttgt tgaaattttt taaacctttg
    10381 tttatttttt tttcttcatt ccgtaactct tctaccttct ttatttactt tctaaaatcc
    10441 aaatacaaaa cataaaaata aataaacaca gagtaaattc ccaaattatt ccatcattaa
    10501 aagatacgag gcgcgtgtaa gttacaggca agcgatccgt ctaagaaacc attattatca
    10561 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
