LOCUS       sequence-367549-        9222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad host range regulated expression of the DddA immunity 
            determinant with a C-terminal fusion to a FLAG epitope (DddAI-FLAG).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9222)
  AUTHORS   Gallagher LA, Velazquez E, Peterson SB, Charity JC, Radey MC, 
            Gebhardt MJ, Hsu F, Shull LM, Cutler KJ, Macareno K, de Moraes MH, 
            Penewit KM, Kim J, Andrade PA, LaFramboise T, Salipante SJ, Reniere 
            ML, de Lorenzo V, Wiggins PA, Dove SL, Mougous JD
  TITLE     Genome-wide protein-DNA interaction site mapping in bacteria using a
            double-stranded DNA-specific cytosine deaminase.
  JOURNAL   Nat Microbiol. 2022 Jun;7(6):844-855. doi: 
            10.1038/s41564-022-01133-9. Epub 2022 Jun 1.
  PUBMED    35650286
REFERENCE   2  (bases 1 to 9222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-022-01133-9"; journalName: "Nat Microbiol"; date: 
            "2022-06"; volume: "7"; issue: "6"; pages: "844-855"
COMMENT     Sequence Label: pMMB67EH-dddAI-FLAG
FEATURES             Location/Qualifiers
     source          1..9222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(38..55)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      208..294
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      386..413
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        432..523
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(524..592,593..1384)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(742..761)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1821..2215
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            2557..2644
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             3883..4854
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             5365..6204
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             6191..7042
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(7319..8401)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8363..8382
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8402..8479)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        join(8709..8714,8715..8730,8731..8737)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    8745..8761
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8750..8772
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             9165..9188
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac
       61 gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg
      121 accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc
      181 atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      241 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
      301 ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca
      361 taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt
      421 ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      481 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      541 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      601 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      661 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      721 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
      781 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      841 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      901 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
      961 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1021 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1081 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1141 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1201 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1261 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1321 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1381 gtaactgtca gaccaagttt actcatatat actttagatt gatttctgaa agcgaccagg
     1441 tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag ccgcccgcat
     1501 tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc ctgctctgcc
     1561 ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag ggtttgctcg
     1621 ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct ggccgccaca
     1681 tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg cttagcggaa
     1741 agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc cagtgcccgt
     1801 cactcccgta ctaactgtca cgaacccctg caataactgt cacgcccccc tgcaataact
     1861 gtcacgaacc cctgcaataa ctgtcacgcc cccaaacctg caaacccagc aggggcgggg
     1921 gctggcgggg tgttggaaaa atccatccat gattatctaa gaataatcca ctaggcgcgg
     1981 ttatcagcgc ccttgtgggg cgctgctgcc cttgcccaat atgcccggcc agaggccgga
     2041 tagctggtct attcgctgcg ctaggctaca caccgcccca ccgctgcgcg gcagggggaa
     2101 aggcgggcaa agcccgctaa accccacacc aaaccccgca gaaatacgct ggagcgcttt
     2161 tagccgcttt agcggccttt ccccctaccc gaagggtggg ggcgcgtgtg cagccccgca
     2221 gggcctgtct cggtcgatca ttcagcccgg ctcatccttc tggcgtggcg gcagaccgaa
     2281 caaggcgcgg tcgtggtcgc gttcaaggta cgcatccatt gccgccatga gccgatcctc
     2341 cggccactcg ctgctgttca ccttggccaa aatcatggcc cccaccagca ccttgcgcct
     2401 tgtttcgttc ttgcgctctt gctgctgttc ccttgcccgc acccgctgaa tttcggcatt
     2461 gattcgcgct cgttgttctt cgagcttggc cagccgatcc gccgccttgt tgctcccctt
     2521 aaccatcttg acaccccatt gttaatgtgc tgtctcgtag gctatcatgg aggcacagcg
     2581 gcggcaatcc cgaccctact ttgtagggga gggcgcactt accggtttct cttcgagaaa
     2641 ctggcctaac ggccaccctt cgggcggtgc gctctccgag ggccattgca tggagccgaa
     2701 aagcaaaagc aacagcgagg cagcatggcg atttatcacc ttacggcgaa aaccggcagc
     2761 aggtcgggcg gccaatcggc cagggccaag gccgactaca tccagcgcga aggcaagtat
     2821 gcccgcgaca tggatgaagt cttgcacgcc gaatccgggc acatgccgga gttcgtcgag
     2881 cggcccgccg actactggga tgctgccgac ctgtatgaac gcgccaatgg gcggctgttc
     2941 aaggaggtcg aatttgccct gccggtcgag ctgaccctcg accagcagaa ggcgctggcg
     3001 tccgagttcg cccagcacct gaccggtgcc gagcgcctgc cgtatacgct ggccatccat
     3061 gccggtggcg gcgagaaccc gcactgccac ctgatgatct ccgagcggat caatgacggc
     3121 atcgagcggc ccgccgctca gtggttcaag cggtacaacg gcaagacccc ggagaagggc
     3181 ggggcacaga agaccgaagc gctcaagccc aaggcatggc ttgagcagac ccgcgaggca
     3241 tgggccgacc atgccaaccg ggcattagag cgggctggcc acgacgcccg cattgaccac
     3301 agaacacttg aggcgcaggg catcgagcgc ctgcccggtg ttcacctggg gccgaacgtg
     3361 gtggagatgg aaggccgggg catccgcacc gaccgggcag acgtggccct gaacatcgac
     3421 accgccaacg cccagatcat cgacttacag gaataccggg aggcaataga ccatgaacgc
     3481 aatcgacaga gtgaagaaat ccagaggcat caacgagtta gcggagcaga tcgaaccgct
     3541 ggcccagagc atggcgacac tggccgacga agcccggcag gtcatgagcc agacccagca
     3601 ggccagcgag gcgcaggcgg cggagtggct gaaagcccag cgccagacag gggcggcatg
     3661 ggtggagctg gccaaagagt tgcgggaggt agccgccgag gtgagcagcg ccgcgcagag
     3721 cgcccggagc gcgtcgcggg ggtggcactg gaagctatgg ctaaccgtga tgctggcttc
     3781 catgatgcct acggtggtgc tgctgatcgc atcgttgctc ttgctcgacc tgacgccact
     3841 gacaaccgag gacggctcga tctggctgcg cttggtggcc cgatgaagaa cgacaggact
     3901 ttgcaggcca taggccgaca gctcaaggcc atgggctgtg agcgcttcga tatcggcgtc
     3961 agggacgcca ccaccggcca gatgatgaac cgggaatggt cagccgccga agtgctccag
     4021 aacacgccat ggctcaagcg gatgaatgcc cagggcaatg acgtgtatat caggcccgcc
     4081 gagcaggagc ggcatggtct ggtgctggtg gacgacctca gcgagtttga cctggatgac
     4141 atgaaagccg agggccggga gcctgccctg gtagtggaaa ccagcccgaa gaactatcag
     4201 gcatgggtca aggtggccga cgccgcaggc ggtgaacttc gggggcagat tgcccggacg
     4261 ctggccagcg agtacgacgc cgacccggcc agcgccgaca gccgccacta tggccgcttg
     4321 gcgggcttca ccaaccgcaa ggacaagcac accacccgcg ccggttatca gccgtgggtg
     4381 ctgctgcgtg aatccaaggg caagaccgcc accgctggcc cggcgctggt gcagcaggct
     4441 ggccagcaga tcgagcaggc ccagcggcag caggagaagg cccgcaggct ggccagcctc
     4501 gaactgcccg agcggcagct tagccgccac cggcgcacgg cgctggacga gtaccgcagc
     4561 gagatggccg ggctggtcaa gcgcttcggt gatgacctca gcaagtgcga ctttatcgcc
     4621 gcgcagaagc tggccagccg gggccgcagt gccgaggaaa tcggcaaggc catggccgag
     4681 gccagcccag cgctggcaga gcgcaagccc ggccacgaag cggattacat cgagcgcacc
     4741 gtcagcaagg tcatgggtct gcccagcgtc cagcttgcgc gggccgagct ggcacgggca
     4801 ccggcacccc gccagcgagg catggacagg ggcgggccag atttcagcat gtagtgcttg
     4861 cgttggtact cacgcctgtt atactatgag tactcacgca cagaaggggg ttttatggaa
     4921 tacgaaaaaa gcgcttcagg gtcggtctac ctgatcaaaa gtgacaaggg ctattggttg
     4981 cccggtggct ttggttatac gtcaaacaag gccgaggctg gccgcttttc agtcgctgat
     5041 atggccagcc ttaaccttga cggctgcacc ttgtccttgt tccgcgaaga caagcctttc
     5101 ggccccggca agtttctcgg tgactgatat gaaagaccaa aaggacaagc agaccggcga
     5161 cctgctggcc agccctgacg ctgtacgcca agcgcgatat gccgagcgca tgaaggccaa
     5221 agggatgcgt cagcgcaagt tctggctgac cgacgacgaa tacgaggcgc tgcgcgagtg
     5281 cctggaagaa ctcagagcgg cgcagggcgg gggtagtgac cccgccagcg cctaaccacc
     5341 aactgcctgc aaaggaggca atcaatggct acccataagc ctatcaatat tctggaggcg
     5401 ttcgcagcag cgccgccacc gctggactac gttttgccca acatggtggc cggtacggtc
     5461 ggggcgctgg tgtcgcccgg tggtgccggt aaatccatgc tggccctgca actggccgca
     5521 cagattgcag gcgggccgga tctgctggag gtgggcgaac tgcccaccgg cccggtgatc
     5581 tacctgcccg ccgaagaccc gcccaccgcc attcatcacc gcctgcacgc ccttggggcg
     5641 cacctcagcg ccgaggaacg gcaagccgtg gctgacggcc tgctgatcca gccgctgatc
     5701 ggcagcctgc ccaacatcat ggccccggag tggttcgacg gcctcaagcg cgccgccgag
     5761 ggccgccgcc tgatggtgct ggacacgctg cgccggttcc acatcgagga agaaaacgcc
     5821 agcggcccca tggcccaggt catcggtcgc atggaggcca tcgccgccga taccgggtgc
     5881 tctatcgtgt tcctgcacca tgccagcaag ggcgcggcca tgatgggcgc aggcgaccag
     5941 cagcaggcca gccggggcag ctcggtactg gtcgataaca tccgctggca gtcctacctg
     6001 tcgagcatga ccagcgccga ggccgaggaa tggggtgtgg acgacgacca gcgccggttc
     6061 ttcgtccgct tcggtgtgag caaggccaac tatggcgcac cgttcgctga tcggtggttc
     6121 aggcggcatg acggcggggt gctcaagccc gccgtgctgg agaggcagcg caagagcaag
     6181 ggggtgcccc gtggtgaagc ctaagaacaa gcacagcctc agccacgtcc ggcacgaccc
     6241 ggcgcactgt ctggcccccg gcctgttccg tgccctcaag cggggcgagc gcaagcgcag
     6301 caagctggac gtgacgtatg actacggcga cggcaagcgg atcgagttca gcggcccgga
     6361 gccgctgggc gctgatgatc tgcgcatcct gcaagggctg gtggccatgg ctgggcctaa
     6421 tggcctagtg cttggcccgg aacccaagac cgaaggcgga cggcagctcc ggctgttcct
     6481 ggaacccaag tgggaggccg tcaccgctga tgccatggtg gtcaaaggta gctatcgggc
     6541 gctggcaaag gaaatcgggg cagaggtcga tagtggtggg gcgctcaagc acatacagga
     6601 ctgcatcgag cgcctttgga aggtatccat catcgcccag aatggccgca agcggcaggg
     6661 gtttcggctg ctgtcggagt acgccagcga cgaggcggac gggcgcctgt acgtggccct
     6721 gaaccccttg atcgcgcagg ccgtcatggg tggcggccag catgtgcgca tcagcatgga
     6781 cgaggtgcgg gcgctggaca gcgaaaccgc ccgcctgctg caccagcggc tgtgtggctg
     6841 gatcgacccc ggcaaaaccg gcaaggcttc catagatacc ttgtgcggct atgtctggcc
     6901 gtcagaggcc agtggttcga ccatgcgcaa gcgccgccag cgggtgcgcg aggcgttgcc
     6961 ggagctggtc gcgctgggct ggacggtaac cgagttcgcg gcgggcaagt acgacatcac
     7021 ccggcccaag gcggcaggct gacccccccc actctattgt aaacaagaca tttttatctt
     7081 ttatattcaa tggcttattt tcctgctaat tggtaatacc atgaaaaata ccatgctcag
     7141 aaaaggctta acaatatttt gaaaaattgc ctactgagcg ctgccgcaca gctccatagg
     7201 ccgctttcct ggctttgctt ccagatgtat gctcttctgc tcccgaacgc cagcaagacg
     7261 tagcccagcg cgtcggccag cttgcaattc gcgctaactt acattaattg cgttgcgctc
     7321 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7381 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
     7441 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
     7501 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac
     7561 atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc
     7621 cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
     7681 cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac
     7741 tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc
     7801 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
     7861 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga
     7921 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
     7981 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
     8041 cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
     8101 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
     8161 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
     8221 actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
     8281 ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
     8341 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
     8401 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
     8461 tgcaccattc gatggtgtca acgtaaatgc cgcttcgcct tcgcgcgcga attgcaagct
     8521 gatccgggct tatcgactgc acggtgcacc aatgcttctg gcgtcaggca gccatcggaa
     8581 gctgtggtat ggctgtgcag gtcgtaaatc actgcataat tcgtgtcgct caaggcgcac
     8641 tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa atattctgaa
     8701 atgagctgtt gacaattaat catcggctcg tataatgtgt ggaattgtga gcggataaca
     8761 atttcacaca ggaaacagaa ttcacgggag gaaagatgta cgcagacgat ttcgacggcg
     8821 aaattgagat cgatgaggtt gatagcttgg tcgagttcct cagcagaaga cctgcatttg
     8881 acgcaaataa ttttgttttg acgtttgagg aaagtggttt tcctcaattg aatatttttg
     8941 caaaaaacga tatcgccgtt gtttattata tggacatcgg cgagaatttc gtttcgaaag
     9001 gcaattctgc gtcaggtggt acggagaagt tttacgagaa taagcttggc ggggaagtag
     9061 acctgtcgaa agattgcgtt gtgagtaaag agcaaatgat tgaagcggca aagcagtttt
     9121 ttgctacgaa gcaacggccc gagcaactga catggagcga gttggactac aaagacgacg
     9181 acgacaaatg aggatcctct agagtcgacc tgcaggcatg ca
//
