LOCUS       sequence-367559-        7174 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Full-length KAP60/SRP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Belanger KD, Kenna MA, Wei S, Davis LI
  TITLE     Genetic and physical interactions between Srp1p and nuclear pore 
            complex proteins Nup1p and Nup2p.
  JOURNAL   J Cell Biol. 1994 Aug;126(3):619-30. doi: 10.1083/jcb.126.3.619.
  PUBMED    8045927
REFERENCE   2  (bases 1 to 7174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb.126.3";
            journalName: "J Cell Biol"; date: "1994-08"; volume: "126"; issue: 
            "3"; pages: "619-30"
COMMENT     Sequence Label: KBB11
FEATURES             Location/Qualifiers
     source          1..7174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        191..471
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             472..1146
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1249..1704)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1386..1407)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1598..1617
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1830..1852
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1844..1861
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1845..1861
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1868..1887
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1868..1886
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1919..1935
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1919..1935
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4356..4372)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4357..4373)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(4401..4419)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4440..4456)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4440..4456)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4453..4475)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4464..4480
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4488..4494,4495..4512,4513..4518))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4533..4554
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4671..4688)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4842..5430)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4922..4941)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5601..6392,6393..6461))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6224..6243
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6462..6566)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    6603..7106
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     7147..7165
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatagatc acgacattac tatatatata atataggaag catttaatag aacagcatcg
      241 taatatatgt gtactttgca gttatgacgc cagatggcag tagtggaaga tattctttat
      301 tgaaaaatag cttgtcacct tacgtacaat cttgatccgg agcttttctt tttttgccga
      361 ttaagaatta attcggtcga aaaaagaaaa ggagagggcc aagagggagg gcattggtga
      421 ctattgagca cgtgagtata cgtgattaag cacacaaagg cagcttggag tatgtctgtt
      481 attaatttca caggtagttc tggtccattg gtgaaagttt gcggcttgca gagcacagag
      541 gccgcagaat gtgctctaga ttccgatgct gacttgctgg gtattatatg tgtgcccaat
      601 agaaagagaa caattgaccc ggttattgca aggaaaattt caagtcttgt aaaagcatat
      661 aaaaatagtt caggcactcc gaaatacttg gttggcgtgt ttcgtaatca acctaaggag
      721 gatgttttgg ctctggtcaa tgattacggc attgatatcg tccaactgca tggagatgag
      781 tcgtggcaag aataccaaga gttcctcggt ttgccagtta ttaaaagact cgtatttcca
      841 aaagactgca acatactact cagtgcagct tcacagaaac ctcattcgtt tattcccttg
      901 tttgattcag aagcaggtgg gacaggtgaa cttttggatt ggaactcgat ttctgactgg
      961 gttggaaggc aagagagccc cgaaagctta cattttatgt tagctggtgg actgacgcca
     1021 gaaaatgttg gtgatgcgct tagattaaat ggcgttattg gtgttgatgt aagcggaggt
     1081 gtggagacaa atggtgtaaa agactctaac aaaatagcaa atttcgtcaa aaatgctaag
     1141 aaataggtta ttactgagta gtatttattt aagtattgtt tgtgcacttg ccgatctatg
     1201 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg
     1261 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     1321 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     1381 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     1441 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     1501 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     1561 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     1621 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     1681 ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg
     1741 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     1801 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     1861 tgaattgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc
     1921 tagaactagt ggatctttca gctgtggata ttcttacttt tttcttgtgt ctttccaatt
     1981 tttttcttat ttttggcgtc ttccgcaaaa tagattaaaa aacccagcaa tacatatata
     2041 acaactatag aacaagtcaa cttctataaa agggaaaaac cccagagaaa gagaatactg
     2101 tacgaactag tacatgaaaa tactattgtg cgaattaaaa gattagaatt tcttatatac
     2161 attaaaagaa aaacttaaaa ataatggatc agataatgaa gatttatctg aataaactta
     2221 tttagttgat ctttttcttc acattcagtt agttaaaatt gaattgttgg ttgacattag
     2281 aaccaaagcc gaaagtatta ccggcatttt gtggagccat agtttcgtct acggcgtctt
     2341 cttcttcacc aaagtaggtt tcaatgattt tgtatgcttt ttcataaatc ttgtcatttt
     2401 cattttgttg acagttgaaa atcttttcca taccaccagc cttttcgata aaatcggcat
     2461 tttcattgat attcaaacca cgagcttctt tgtcagcttc acccatcttt aaaatatttt
     2521 caagagcatc taaggtaact tcaattattc tgttgtcagc aatttctagc aaatcacaca
     2581 atggttttat acacccttga gatactaaat atcttatgat atctggtctt tgtaaaccac
     2641 ctgaagaggc attggaaata gcccaacaag cttctttttt agttttatat tctgcaactt
     2701 ccaaaagttt aactaatgga ggaatcaagt tcgcgtcaat taccgcttga atttgttcag
     2761 tattaccagc cgtaatattg gaaatggtcc aacacgcttc tttcttgata ttttcttttg
     2821 gagagcttag cagaagtctt aacgcaggta agacaccagc atttataacg acctgagtct
     2881 gtaagtcatt accagtgact atattaccta cagctcttaa agcaggagtc tggactaaag
     2941 tcgattcatg gctcagtaat tcaacaagtc ttttaggaat tctaacatcg atcactgctt
     3001 gaatagcttc ttgtggtccg tcagatagat aagagatagc ccaacaagca tcaactaaag
     3061 tttcagtgtc catcgaatag attaatttcg ctaaggttgg caacgcttgt gagaccactg
     3121 accaatctgg ttgtggtttt ttacccctgc ataaattgga taaagtccac gtagcggtcc
     3181 tgatcaaaga tggtttattg gagttaaaaa gacccaaaat tggctccatg gcattacatt
     3241 gtaaaacgta gtctctgtag tcagttgaat cacctgcaac gttacctaag gcccaaatgg
     3301 cttgttcttt aacttcaacg gagccggtat ataatagttg aatgaaaaga ggtacagcgt
     3361 cagcatcaac aaccactttt gtttgagcag atgtacctga tgcaatgtta gtcaaagccc
     3421 aagcagcctc caattgtaac atttcaggtt ggttttcacg cataaattct actaatcttg
     3481 gaacaacacc ggcttgaatg acgacatcaa ttggagggcg gtgttctcta gacaaaattt
     3541 gtctaaactt aacagtagca ctcaattgct cttgcatatc atcagagtta agttgctgag
     3601 tcatttgtgg tagttcttgc tgcaactggc tgtaaaattg ttggtccgca gaaacggagc
     3661 tctcatcttc ttcatcagaa tcagcgccat cagttggggg aataaagttt cttcttttgg
     3721 ccaaagcttc atctcttttt gcttttctta attcgacctg ttgtgtatct ctacgacgac
     3781 gaagttcatc tgcagagaat ctgcctttat tcttaaagtt tgttcgtcta tattcgggaa
     3841 cgaacttgct cgtggaagaa tctgtaccat tatccatatt tattaatttg ttatgtagaa
     3901 gggaaggtat atcaagtgaa caaaaaacaa aatagaaaga tcgttctata aaataacgat
     3961 gtaacttcaa ttaagtggga aatgtttcag tcttaacaaa ataaagcctg aagatgtgag
     4021 tgtaaaagga ggcttatagt tggtcctgtt ccttattccg tcagtgaatg cgggttttcc
     4081 ttaccttaca agtcgcttct tgagcagaat atgattaatc actaaattcg tatctgaacc
     4141 gtttccttcc tagtccttcc ccatcaactc ttgaagaaaa aagaaaagaa gaaaattcgt
     4201 taatggtgat gtaatctctt ttgcgcttcg aggaaaaatg gacaagtatc acttagatag
     4261 ttcgactggc aaactcttcg gtgcattgga attgattact attgaattaa ttcggtcgat
     4321 taagttgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct cgaggggggg
     4381 cccggtacca gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg
     4441 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     4501 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     4561 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     4621 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     4681 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4741 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4801 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4861 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4921 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4981 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5041 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5101 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5161 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5221 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5281 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5341 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5401 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5461 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     5521 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     5581 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     5641 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     5701 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     5761 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     5821 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     5881 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     5941 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6001 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     6061 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6121 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     6181 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     6241 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     6301 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6361 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     6421 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     6481 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     6541 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtccttt
     6601 tcatcacgtg ctataaaaat aattataatt taaatttttt aatataaata tataaattaa
     6661 aaatagaaag taaaaaaaga aattaaagaa aaaatagttt ttgttttccg aagatgtaaa
     6721 agactctagg gggatcgcca acaaatacta ccttttatct tgctcttcct gctctcaggt
     6781 attaatgccg aattgtttca tcttgtctgt gtagaagacc acacacgaaa atcctgtgat
     6841 tttacatttt acttatcgtt aatcgaatgt atatctattt aatctgcttt tcttgtctaa
     6901 taaatatata tgtaaagtac gctttttgtt gaaatttttt aaacctttgt ttattttttt
     6961 ttcttcattc cgtaactctt ctaccttctt tatttacttt ctaaaatcca aatacaaaac
     7021 ataaaaataa ataaacacag agtaaattcc caaattattc catcattaaa agatacgagg
     7081 cgcgtgtaag ttacaggcaa gcgatccgtc taagaaacca ttattatcat gacattaacc
     7141 tataaaaata ggcgtatcac gaggcccttt cgtc
//
