LOCUS       sequence-367692-        7738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optically controlled eDrIR1 expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   Leopold AV, Thankachan S, Yang C, Gerashchenko D, Verkhusha VV
  TITLE     A general approach for engineering RTKs optically controlled with 
            far-red light.
  JOURNAL   Nat Methods. 2022 Jul;19(7):871-880. doi: 
            10.1038/s41592-022-01517-z. Epub 2022 Jun 9.
  PUBMED    35681062
REFERENCE   2  (bases 1 to 7738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-022-01517-z"; journalName: "Nat Methods"; date: 
            "2022-07"; volume: "19"; issue: "7"; pages: "871-880"
COMMENT     Sequence Label: eDrIR1
FEATURES             Location/Qualifiers
     source          1..7738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     sig_peptide     597..656
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             660..1361
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="SKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQ
                     TAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGE
                     IKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEG
                     RHSTGGMDELYK"
     primer_bind     complement(799..817)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1067..1086
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4526..4647
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4563..4582)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4617..4636
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4654..5109)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4791..4812)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5003..5022
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5136..5240
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5242..5599
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5242..5433
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5265..5285)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5450..5585
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5634..6428
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5634..6428
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5688..5707)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6298..6317
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6616..6635)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6660..6707
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7036..7624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7525..7544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgg
      601 agacagacac actcctgcta tgggtactgc tgctctgggt tccaggttcc actggtgaca
      661 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
      721 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
      781 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
      841 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
      901 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
      961 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     1021 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     1081 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     1141 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     1201 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     1261 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     1321 agggccgcca ctccaccggc ggcatggacg agctgtacaa ggaattcagt gctggtagtg
     1381 ctggtagtgc tggcaccggt atgagccggg acccgttgcc cttttttcca ccgctttacc
     1441 ttggtggccc ggaaattacc accgagaact gcgagcgcga gccgattcat attcccggca
     1501 gcatccagcc gcacggcgcc ctgctcactg ccgacgggca cagcggcgag gtgctccaga
     1561 tgagcctcaa cgcggccact tttctgggac aggaacccac agtgctgcgc ggacagaccc
     1621 tcgccgcact gctgcccgag cagtggcccg cgctgcaagc ggccctgccc cccggctgcc
     1681 ccgacgccct gcaataccgc gcaacgctgg actggcctgc cgccgggcac ctttcgctga
     1741 cggtgcaccg ggtcggcgag ttgctgattc tggagttcga gccgacggag gcctgggaca
     1801 gcaccgggcc gcacgcgctg cgcaacgcga tgttcgcgct cgaaagtgcc cccaacctgc
     1861 gggcgctggc cgaggtggcg acccagacgg tccgcgagct gacgggcttt gaccgggtga
     1921 tgctctacaa atttgccccc gacgccaccg gcgaagtgat tgccgaggcc cgccgtgagg
     1981 ggctgcacgc ctttctgggc caccgttttc ccgcgtcgga cattccggcg caggcccgcg
     2041 cgctctacac ccggcacctg ctgcgcctga ccgccgacac ccgcgccgcc gccgtgccgc
     2101 tcgatcccgt cctcaacccg cagacgaatg cgcccacccc gctgggcggc gccgtgctgc
     2161 gcgccacctc gcccatgcac atgcagtacc tgcggaacat gggcgtcggg tcgagcctgt
     2221 cggtgtcggt ggtggtcggc ggccagctct ggggcctgat cgcctgccac caccagacgc
     2281 cctacgtgtt gccgcccgac ctgcgaacca cgctcgaata cctgggccgc ttgctgagcc
     2341 tgcaagttca ggtcaaggaa gcggcggacg tggcggcctt tcgccagagc ctgcgggagc
     2401 accacgcgcg ggtggccctc gcggcggcgc actcgctctc gccgcacgac accctcagtg
     2461 acccggcgct tgacctgctg ggcctgatgc gggccggggg cctgattctg cgtttcgagg
     2521 gccgctggca gacgttgggt gaagtgccgc ctgccccggc ggtggacgcg ctgctggcgt
     2581 ggctcgaaac ccagccgggc gccctggtcc agaccgacgc gctaggccaa ctgtggcccg
     2641 ccggcgccga tctcgccccc agcgcagcgg gcctgctcgc catcagcgtg ggcgagggct
     2701 ggtcggagtg cctcgtctgg ctgcggcccg aactgcggct ggaggtcgcc tggggcgggg
     2761 ccactcctga ccaggcgaaa gacgacctcg ggccgcgcca ctcattcgac acctacctcg
     2821 aagaaaaacg cggctacgcc gagccctggc atcccggcga aatcgaggag gcgcaggatc
     2881 tacgtgacac attgaccggg gcgctgggcg agcagctcga gtccatcgtc tctgcggtgg
     2941 ttggcattct gctggtcgtg gtcttggggg tggtctttgg gatcctctct agaagaaaga
     3001 ggcagccaga tgggccgctg ggaccgattt acgcttcttc aaaccctgag tatctcagtg
     3061 ccagtgatgt gtttccatgc tctgtgtacg tgccggacga gtgggaggtg tctcgagaga
     3121 agatcaccct ccttcgagag ctggggcagg gctccttcgg catggtgtat gagggcaatg
     3181 ccagggacat catcaagggt gaggcagaga cccgcgtggc ggtgaagacg gtcaacgagt
     3241 cagccagtct ccgagagcgg attgagttcc tcaatgaggc ctcggtcatg aagggcttca
     3301 cctgccatca cgtggtgcgc ctcctgggag tggtgtccaa gggccagccc acgctggtgg
     3361 tgatggagct gatggctcac ggagacctga agagctacct ccgttctctg cggccagagg
     3421 ctgagaataa tcctggccgc cctcccccta cccttcaaga gatgattcag atggcggcag
     3481 agattgctga cgggatggcc tacctgaacg ccaagaagtt tgtgcatcgg gacctggcag
     3541 cgagaaactg catggtcgcc catgatttta ctgtcaaaat tggagacttt ggaatgacca
     3601 gagacatcta tgaaacggat tactaccgga aagggggcaa gggtctgctc cctgtacggt
     3661 ggatggcacc ggagtccctg aaggatgggg tcttcaccac ttcttctgac atgtggtcct
     3721 ttggcgtggt cctttgggaa atcaccagct tggcagaaca gccttaccaa ggcctgtcta
     3781 atgaacaggt gttgaaattt gtcatggatg gagggtatct ggatcaaccc gacaactgtc
     3841 cagagagagt cactgacctc atgcgcatgt gctggcaatt caaccccaag atgaggccaa
     3901 ccttcctgga gattgtcaac ctgctcaagg acgacctgca ccccagcttt ccagaggtgt
     3961 cgttcttcca cagcgaggag aacaaggctc ccgagagtga ggagctggag atggagtttg
     4021 aggacatgga gaatgtgccc ctggaccgtt cctcgcactg tcagagggag gaggcggggg
     4081 gccgggatgg agggtcctcg ctgggtttca agcggagcta cgaggaacac atcccttaca
     4141 cacacatgaa cggaggcaag aaaaacgggc ggattctgac cttgcctcgg tccaatcctt
     4201 cctctagagg cggtggtggc agcggaggag gggggagcgg cggcggtggc agtggcggcg
     4261 gcggtagtcg cagccgcttt gtgaaaaaag atagcgccgg cagtgcgggt agtgcgggga
     4321 gcgccggtag ctatctggcg aacgaaattc tgtggggcag cgctggtagc gcaggaagcg
     4381 ccggcagtgc agggttttgc tatgaaaacg aagtggcgct gtcataatca gccataccac
     4441 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     4501 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     4561 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4621 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa
     4681 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     4741 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     4801 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     4861 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     4921 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     4981 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     5041 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     5101 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5161 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5221 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     5281 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5341 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     5401 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     5461 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5521 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     5581 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
     5641 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     5701 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     5761 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     5821 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     5881 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     5941 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     6001 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     6061 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     6121 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     6181 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     6241 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     6301 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     6361 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     6421 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     6481 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     6541 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     6601 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     6661 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
     6721 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     6781 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     6841 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
     6901 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6961 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7021 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7081 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7141 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7201 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7261 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7321 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7381 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7441 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7501 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7561 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7621 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7681 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
