LOCUS       sequence-367723-       11395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human CD79A as YFP fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11395)
  AUTHORS   Sevdali E, Block V, Lataretu M, Li H, Smulski CR, Briem JS, Heitz Y,
            Fischer B, Ramirez NJ, Grimbacher B, Jack HM, Voll RE, Holzer M, 
            Schneider P, Eibel H
  TITLE     BAFFR activates PI3K/AKT signaling in human naive but not in 
            switched memory B cells through direct interactions with B cell 
            antigen receptors.
  JOURNAL   Cell Rep. 2022 Jun 28;39(13):111019. doi: 
            10.1016/j.celrep.2022.111019.
  PUBMED    35767961
REFERENCE   2  (bases 1 to 11395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-06-28"; volume: "39(13):111019. doi"; pages: " 
            10.1016/j.celrep.2022.111019"
COMMENT     Sequence Label: pNL-CEF-CD79A-YFP
FEATURES             Location/Qualifiers
     source          1..11395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             377..634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      1261..1396
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2092..2136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2406..2709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2719..3901
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2950..3892
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3849..3869
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     3923..3942
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3923..3941
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4660..4669
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4666..4668,4669..4671,4672..5385)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4711..4732)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4972..4991)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5319..5340
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(5413..5431)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5414..5431)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5451..5468)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             5645..6278
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7919..7936)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8090..8678)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8170..8189)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8849..9640,9641..9709))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9472..9491
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9710..9814)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9882..9900
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9938..9960)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10060..10079
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatat gactccgccc
     1261 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     1321 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     1381 ggcttttttg gaggcctagg cttttgcaaa tgcataaaag aaaagggggg attggggggt
     1441 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     1501 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1861 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatttggaa taacatgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctgt agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatctagtta actagatcta
     2401 gagtccgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     2461 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     2521 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     2581 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     2641 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     2701 attacccggc atgcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     2761 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     2821 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     2881 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     2941 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     3001 ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc ttgatcccga
     3061 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
     3121 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
     3181 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
     3241 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
     3301 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     3361 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     3421 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     3481 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     3541 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     3601 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     3661 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
     3721 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     3781 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     3841 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     3901 aggcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
     3961 gagctcgcca ccatgcctgg gggtccagga gtcctccaag ctctgcctgc caccatcttc
     4021 ctcctcttcc tgctgtctgc tgtctacctg ggccctgggt gccaggccct gtggatgcac
     4081 aaggtcccag catcattgat ggtgagcctg ggggaagacg cccacttcca atgcccgcac
     4141 aatagcagca acaacgccaa cgtcacctgg tggcgcgtcc tccatggcaa ctacacgtgg
     4201 ccccctgagt tcttgggccc gggcgaggac cccaatggta cgctgatcat ccagaatgtg
     4261 aacaagagcc atgggggcat atacgtgtgc cgggtccagg agggcaacga gtcataccag
     4321 cagtcctgcg gcacctacct ccgcgtgcgc cagccgcccc ccaggccctt cctggacatg
     4381 ggggagggca ccaagaaccg aatcatcaca gccgagggga tcatcctcct gttctgcgcg
     4441 gtggtgcctg ggacgctgct gctgttcagg aaacgatggc agaacgagaa gctcgggttg
     4501 gatgccgggg atgaatatga agatgaaaac ctttatgaag gcctgaacct ggacgactgc
     4561 tccatgtatg aggacatctc ccggggcctc cagggcacct accaggatgt gggcagcctc
     4621 aacataggag atgtccagct ggagaagccg gacccggtcg ccaccatggt gagcaagggc
     4681 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     4741 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     4801 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
     4861 ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     4921 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     4981 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     5041 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     5101 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     5161 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     5221 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     5281 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     5341 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct
     5401 agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt
     5461 gccttctagt tgccaggagc aatcacaagt agcaatacag cagctaacaa tgctgcttgt
     5521 gcctggctag aagcacaaga ggaggaagag gtgggttttc cagtcacacc tcaggtacct
     5581 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5641 ggactggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
     5701 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg ggtcagatat
     5761 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     5821 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg aatggatgac
     5881 cctgagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     5941 cgagagctgc atccggagta cttcaagaac tgctgacatc gagcttgcta caagggactt
     6001 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     6061 agatgctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     6121 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6181 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6241 cctcagaccc ttttagtcag tgtggaaaat ctctagcacc caggaggtag aggttgcagt
     6301 gagccaagat cgcgccactg cattccagcc tgggcaagaa aacaagactg tctaaaataa
     6361 taataataag ttaagggtat taaatatatt tatacatgga ggtcataaaa atatatatat
     6421 ttgggctggg cgcagtggct cacacctgcg cccggccctt tgggaggccg aggcaggtgg
     6481 atcacctgag tttgggagtt ccagaccagc ctgaccaaca tggagaaacc ccttctctgt
     6541 gtatttttag tagattttat tttatgtgta ttttattcac aggtatttct ggaaaactga
     6601 aactgttttt cctctactct gataccacaa gaatcatcag cacagaggaa gacttctgtg
     6661 atcaaatgtg gtgggagagg gaggttttca ccagcacatg agcagtcagt tctgccgcag
     6721 actcggcggg tgtccttcgg ttcagttcca acaccgcctg cctggagaga ggtcagacca
     6781 cagggtgagg gctcagtccc caagacataa acacccaaga cataaacacc caacaggtcc
     6841 accccgcctg ctgcccaggc agagccgatt caccaagacg ggaattagga tagagaaaga
     6901 gtaagtcaca cagagccggc tgtgcgggag aacggagttc tattatgact caaatcagtc
     6961 tccccaagca ttcggggatc agagttttta aggataactt agtgtgtagg gggccagtga
     7021 gttggagatg aaagcgtagg gagtcgaagg tgtccttttg cgccgagtca gttcctgggt
     7081 gggggccaca agatcggatg agccagttta tcaatccggg ggtgccagct gatccatgga
     7141 gtgcagggtc tgcaaaatat ctcaagcact gattgatctt aggttttaca atagtgatgt
     7201 taccccagga acaatttggg gaaggtcaga atcttgtagc ctgtagctgc atgactccta
     7261 aaccataatt tcttttttgt tttttttttt tttatttttg agacagggtc tcactctgtc
     7321 acctaggctg gagtgcagtg gtgcaatcac agctcactgc agcctcaacg tcgtaagctc
     7381 aagcgatcct cccacctcag cctgcctggt agctgagact acaagcgacg ccccagttaa
     7441 tttttgtatt tttggtagag gcagcgtttt gccgtgtggc cctggctggt ctcgaactcc
     7501 tgggctcaag tgatccagcc tcagcctccc aaagtgctgg gacaaccggg gccagtcact
     7561 gcacctggcc ctaaaccata atttctaatc ttttggctaa tttgttagtc ctacaaaggc
     7621 agtctagtcc ccaggcaaaa agggggtttg tttcgggaaa gggctgttac tgtctttgtt
     7681 tcaaactata aactaagttc ctcctaaact tagttcggcc tacacccagg aatgaacaag
     7741 gagagcttgg aggttagaag cacgatggaa ttggttaggt cagatctctt tcactgtctg
     7801 agttataatt ttgcaatggt ggttcaaaga ctgcccgctt ctgacaccag tcgctgcatt
     7861 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7921 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7981 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8041 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8101 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8161 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8221 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8281 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8341 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8401 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8461 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8521 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8581 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8641 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8701 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8761 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8821 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8881 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8941 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9001 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9061 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9121 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9181 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9241 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9301 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9361 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9421 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9481 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9541 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9601 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9661 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9721 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9781 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9841 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     9901 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     9961 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    10021 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
    10081 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    10141 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    10201 cttcgctatt acgccagggg aggcagagat tgcagtaagc tgagatcgca gcactgcact
    10261 ccagcctggg cgacagagta agactctgtc tcaaaaataa aataaataaa tcaatcagat
    10321 attccaatct tttcctttat ttatttattt attttctatt ttggaaacac agtccttcct
    10381 tattccagaa ttacacatat attctatttt tctttatatg ctccagtttt ttttagacct
    10441 tcacctgaaa tgtgtgtata caaaatctag gccagtccag cagagcctaa aggtaaaaaa
    10501 taaaataata aaaaataaat aaaatctagc tcactccttc acatcaaaat ggagatacag
    10561 ctgttagcat taaataccaa ataacccatc ttgtcctcaa taattttaag cgcctctctc
    10621 caccacatct aactcctgtc aaaggcatgt gccccttccg ggcgctctgc tgtgctgcca
    10681 accaactggc atgtggactc tgcagggtcc ctaactgcca agccccacag tgtgccctga
    10741 ggctgcccct tccttctagc ggctgccccc actcggcttt gctttcccta gtttcagtta
    10801 cttgcgttca gccaaggtct gaaactaggt gcgcacagag cggtaagact gcgagagaaa
    10861 gagaccagct ttacaggggg tttatcacag tgcaccctga cagtcgtcag cctcacaggg
    10921 ggtttatcac attgcaccct gacagtcgtc agcctcacag ggggtttatc acagtgcacc
    10981 cttacaatca ttccatttga ttcacaattt ttttagtctc tactgtgcct aacttgtaag
    11041 ttaaatttga tcagaggtgt gttcccagag gggaaaacag tatatacagg gttcagtact
    11101 atcgcatttc aggcctccac ctgggtcttg gaatgtgtcc cccgaggggt gatgactacc
    11161 tcagttggat ctccacaggt cacagtgaca caagataacc aagacacctc ccaaggctac
    11221 cacaatgggc cgccctccac gtgcacatgg ccggaggaac tgccatgtcg gaggtgcaag
    11281 cacacctgcg catcagagtc cttggtgtgg agggagggac cagcgcagct tccagccatc
    11341 cacctgatga acagaaccta gggaaagccc cagttctact tacaccagga aaggc
//
